Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10920

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10910 and RALBFv3_RS10915 are separated by 151 nucleotidesRALBFv3_RS10915 and RALBFv3_RS10920 are separated by 87 nucleotidesRALBFv3_RS10920 and RALBFv3_RS10925 are separated by 19 nucleotidesRALBFv3_RS10925 and RALBFv3_RS10930 are separated by 99 nucleotides RALBFv3_RS10910: RALBFv3_RS10910 - glutaredoxin 3, at 2,414,824 to 2,415,081 _RS10910 RALBFv3_RS10915: RALBFv3_RS10915 - protein-export chaperone SecB, at 2,415,233 to 2,415,754 _RS10915 RALBFv3_RS10920: RALBFv3_RS10920 - glycerol-3-phosphate dehydrogenase, at 2,415,842 to 2,416,858 _RS10920 RALBFv3_RS10925: RALBFv3_RS10925 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 2,416,878 to 2,417,348 _RS10925 RALBFv3_RS10930: RALBFv3_RS10930 - amidophosphoribosyltransferase, at 2,417,448 to 2,418,170 _RS10930 Position (kb) 2415 2416 2417Strain fitness (log2 ratio) -1 0 1 2at 2414.842 kb on + strandat 2414.842 kb on + strandat 2414.843 kb on - strandat 2414.843 kb on - strandat 2414.996 kb on - strand, within RALBFv3_RS10910at 2414.996 kb on - strand, within RALBFv3_RS10910at 2415.162 kb on + strandat 2415.162 kb on + strandat 2415.162 kb on + strandat 2415.163 kb on - strandat 2415.167 kb on + strandat 2415.206 kb on + strandat 2415.207 kb on - strandat 2415.752 kb on + strandat 2415.752 kb on + strandat 2415.753 kb on - strandat 2415.753 kb on - strandat 2416.199 kb on - strand, within RALBFv3_RS10920at 2416.921 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,414,842 + -0.0
2,414,842 + +0.2
2,414,843 - -0.9
2,414,843 - +1.1
2,414,996 - RALBFv3_RS10910 0.67 -1.7
2,414,996 - RALBFv3_RS10910 0.67 -0.1
2,415,162 + -0.8
2,415,162 + +0.3
2,415,162 + -0.5
2,415,163 - -0.6
2,415,167 + -0.9
2,415,206 + +1.1
2,415,207 - +2.1
2,415,752 + +0.2
2,415,752 + -0.1
2,415,753 - +0.6
2,415,753 - -0.1
2,416,199 - RALBFv3_RS10920 0.35 -0.7
2,416,921 - +1.1

Or see this region's nucleotide sequence