Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10885 and RALBFv3_RS10890 are separated by 66 nucleotides RALBFv3_RS10890 and RALBFv3_RS10895 are separated by 70 nucleotides RALBFv3_RS10895 and RALBFv3_RS10900 are separated by 135 nucleotides
RALBFv3_RS10885: RALBFv3_RS10885 - bacterioferritin, at 2,410,348 to 2,410,827
_RS10885
RALBFv3_RS10890: RALBFv3_RS10890 - molybdopterin-synthase adenylyltransferase MoeB, at 2,410,894 to 2,411,643
_RS10890
RALBFv3_RS10895: RALBFv3_RS10895 - S41 family peptidase, at 2,411,714 to 2,413,330
_RS10895
RALBFv3_RS10900: RALBFv3_RS10900 - 2,3-diphosphoglycerate-dependent phosphoglycerate mutase, at 2,413,466 to 2,414,212
_RS10900
Position (kb)
2411
2412
2413
2414 Strain fitness (log2 ratio)
-2
-1
0
1 at 2410.792 kb on + strand at 2412.244 kb on + strand, within RALBFv3_RS10895 at 2412.246 kb on + strand, within RALBFv3_RS10895 at 2412.505 kb on - strand, within RALBFv3_RS10895 at 2412.505 kb on - strand, within RALBFv3_RS10895 at 2412.713 kb on - strand, within RALBFv3_RS10895 at 2412.826 kb on - strand, within RALBFv3_RS10895 at 2413.419 kb on + strand at 2413.419 kb on + strand at 2413.420 kb on - strand at 2413.537 kb on - strand at 2413.944 kb on + strand, within RALBFv3_RS10900 at 2414.298 kb on - strand at 2414.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,410,792 + -0.2 2,412,244 + RALBFv3_RS10895 0.33 +0.1 2,412,246 + RALBFv3_RS10895 0.33 -0.7 2,412,505 - RALBFv3_RS10895 0.49 +1.1 2,412,505 - RALBFv3_RS10895 0.49 -1.1 2,412,713 - RALBFv3_RS10895 0.62 -2.7 2,412,826 - RALBFv3_RS10895 0.69 -1.8 2,413,419 + -0.3 2,413,419 + -0.5 2,413,420 - +0.3 2,413,537 - +1.1 2,413,944 + RALBFv3_RS10900 0.64 +1.1 2,414,298 - -0.3 2,414,300 - -0.9
Or see this region's nucleotide sequence