Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10860 and RALBFv3_RS10865 are separated by 108 nucleotides RALBFv3_RS10865 and RALBFv3_RS10870 overlap by 32 nucleotides RALBFv3_RS10870 and RALBFv3_RS10875 are separated by 40 nucleotides RALBFv3_RS10875 and RALBFv3_RS10880 are separated by 210 nucleotides RALBFv3_RS10880 and RALBFv3_RS10885 are separated by 140 nucleotides
RALBFv3_RS10860: RALBFv3_RS10860 - glutathione synthase, at 2,406,144 to 2,407,118
_RS10860
RALBFv3_RS10865: RALBFv3_RS10865 - PTS fructose transporter subunit IIA, at 2,407,227 to 2,407,682
_RS10865
RALBFv3_RS10870: RALBFv3_RS10870 - HPr family phosphocarrier protein, at 2,407,651 to 2,407,920
_RS10870
RALBFv3_RS10875: RALBFv3_RS10875 - phosphoenolpyruvate--protein phosphotransferase, at 2,407,961 to 2,409,721
_RS10875
RALBFv3_RS10880: RALBFv3_RS10880 - (2Fe-2S)-binding protein, at 2,409,932 to 2,410,207
_RS10880
RALBFv3_RS10885: RALBFv3_RS10885 - bacterioferritin, at 2,410,348 to 2,410,827
_RS10885
Position (kb)
2407
2408
2409
2410 Strain fitness (log2 ratio)
-2
-1
0
1 at 2406.972 kb on + strand, within RALBFv3_RS10860 at 2407.116 kb on + strand at 2407.193 kb on + strand at 2407.194 kb on - strand at 2407.534 kb on - strand, within RALBFv3_RS10865 at 2407.594 kb on + strand, within RALBFv3_RS10865 at 2407.595 kb on - strand, within RALBFv3_RS10865 at 2407.650 kb on + strand at 2407.651 kb on - strand at 2407.651 kb on - strand at 2407.651 kb on - strand at 2407.651 kb on - strand at 2407.680 kb on + strand at 2407.680 kb on + strand at 2408.020 kb on + strand at 2408.054 kb on + strand at 2409.168 kb on + strand, within RALBFv3_RS10875 at 2409.776 kb on - strand at 2410.360 kb on - strand at 2410.360 kb on - strand at 2410.421 kb on - strand, within RALBFv3_RS10885 at 2410.480 kb on + strand, within RALBFv3_RS10885 at 2410.480 kb on + strand, within RALBFv3_RS10885 at 2410.480 kb on + strand, within RALBFv3_RS10885
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,406,972 + RALBFv3_RS10860 0.85 +0.5 2,407,116 + -1.4 2,407,193 + -0.4 2,407,194 - -0.6 2,407,534 - RALBFv3_RS10865 0.67 -0.0 2,407,594 + RALBFv3_RS10865 0.80 +1.1 2,407,595 - RALBFv3_RS10865 0.81 +0.7 2,407,650 + +0.2 2,407,651 - +0.5 2,407,651 - -2.0 2,407,651 - +0.3 2,407,651 - +0.2 2,407,680 + -0.2 2,407,680 + +0.1 2,408,020 + +1.1 2,408,054 + +1.1 2,409,168 + RALBFv3_RS10875 0.69 -1.7 2,409,776 - -0.2 2,410,360 - +0.0 2,410,360 - +1.1 2,410,421 - RALBFv3_RS10885 0.15 -0.6 2,410,480 + RALBFv3_RS10885 0.28 +0.1 2,410,480 + RALBFv3_RS10885 0.28 -0.1 2,410,480 + RALBFv3_RS10885 0.28 +0.5
Or see this region's nucleotide sequence