Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10875

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10860 and RALBFv3_RS10865 are separated by 108 nucleotidesRALBFv3_RS10865 and RALBFv3_RS10870 overlap by 32 nucleotidesRALBFv3_RS10870 and RALBFv3_RS10875 are separated by 40 nucleotidesRALBFv3_RS10875 and RALBFv3_RS10880 are separated by 210 nucleotidesRALBFv3_RS10880 and RALBFv3_RS10885 are separated by 140 nucleotides RALBFv3_RS10860: RALBFv3_RS10860 - glutathione synthase, at 2,406,144 to 2,407,118 _RS10860 RALBFv3_RS10865: RALBFv3_RS10865 - PTS fructose transporter subunit IIA, at 2,407,227 to 2,407,682 _RS10865 RALBFv3_RS10870: RALBFv3_RS10870 - HPr family phosphocarrier protein, at 2,407,651 to 2,407,920 _RS10870 RALBFv3_RS10875: RALBFv3_RS10875 - phosphoenolpyruvate--protein phosphotransferase, at 2,407,961 to 2,409,721 _RS10875 RALBFv3_RS10880: RALBFv3_RS10880 - (2Fe-2S)-binding protein, at 2,409,932 to 2,410,207 _RS10880 RALBFv3_RS10885: RALBFv3_RS10885 - bacterioferritin, at 2,410,348 to 2,410,827 _RS10885 Position (kb) 2407 2408 2409 2410Strain fitness (log2 ratio) -2 -1 0 1at 2406.972 kb on + strand, within RALBFv3_RS10860at 2407.116 kb on + strandat 2407.193 kb on + strandat 2407.194 kb on - strandat 2407.534 kb on - strand, within RALBFv3_RS10865at 2407.594 kb on + strand, within RALBFv3_RS10865at 2407.595 kb on - strand, within RALBFv3_RS10865at 2407.650 kb on + strandat 2407.651 kb on - strandat 2407.651 kb on - strandat 2407.651 kb on - strandat 2407.651 kb on - strandat 2407.680 kb on + strandat 2407.680 kb on + strandat 2408.020 kb on + strandat 2408.054 kb on + strandat 2409.168 kb on + strand, within RALBFv3_RS10875at 2409.776 kb on - strandat 2410.360 kb on - strandat 2410.360 kb on - strandat 2410.421 kb on - strand, within RALBFv3_RS10885at 2410.480 kb on + strand, within RALBFv3_RS10885at 2410.480 kb on + strand, within RALBFv3_RS10885at 2410.480 kb on + strand, within RALBFv3_RS10885

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,406,972 + RALBFv3_RS10860 0.85 +0.5
2,407,116 + -1.4
2,407,193 + -0.4
2,407,194 - -0.6
2,407,534 - RALBFv3_RS10865 0.67 -0.0
2,407,594 + RALBFv3_RS10865 0.80 +1.1
2,407,595 - RALBFv3_RS10865 0.81 +0.7
2,407,650 + +0.2
2,407,651 - +0.5
2,407,651 - -2.0
2,407,651 - +0.3
2,407,651 - +0.2
2,407,680 + -0.2
2,407,680 + +0.1
2,408,020 + +1.1
2,408,054 + +1.1
2,409,168 + RALBFv3_RS10875 0.69 -1.7
2,409,776 - -0.2
2,410,360 - +0.0
2,410,360 - +1.1
2,410,421 - RALBFv3_RS10885 0.15 -0.6
2,410,480 + RALBFv3_RS10885 0.28 +0.1
2,410,480 + RALBFv3_RS10885 0.28 -0.1
2,410,480 + RALBFv3_RS10885 0.28 +0.5

Or see this region's nucleotide sequence