Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10790 and RALBFv3_RS10795 are separated by 25 nucleotides RALBFv3_RS10795 and RALBFv3_RS10800 are separated by 78 nucleotides RALBFv3_RS10800 and RALBFv3_RS10805 are separated by 103 nucleotides
RALBFv3_RS10790: RALBFv3_RS10790 - sensor histidine kinase, at 2,390,757 to 2,392,754
_RS10790
RALBFv3_RS10795: RALBFv3_RS10795 - sigma-54-dependent Fis family transcriptional regulator, at 2,392,780 to 2,394,096
_RS10795
RALBFv3_RS10800: RALBFv3_RS10800 - TlpA family protein disulfide reductase, at 2,394,175 to 2,394,723
_RS10800
RALBFv3_RS10805: RALBFv3_RS10805 - O-acetyl-ADP-ribose deacetylase, at 2,394,827 to 2,395,342
_RS10805
Position (kb)
2392
2393
2394
2395 Strain fitness (log2 ratio)
-2
-1
0
1 at 2391.834 kb on + strand, within RALBFv3_RS10790 at 2392.339 kb on + strand, within RALBFv3_RS10790 at 2392.340 kb on - strand, within RALBFv3_RS10790 at 2392.405 kb on + strand, within RALBFv3_RS10790 at 2392.879 kb on + strand at 2392.879 kb on + strand at 2392.880 kb on - strand at 2393.686 kb on + strand, within RALBFv3_RS10795 at 2394.067 kb on + strand at 2394.243 kb on - strand, within RALBFv3_RS10800 at 2394.374 kb on + strand, within RALBFv3_RS10800 at 2394.375 kb on - strand, within RALBFv3_RS10800 at 2394.375 kb on - strand, within RALBFv3_RS10800 at 2394.375 kb on - strand, within RALBFv3_RS10800 at 2394.401 kb on + strand, within RALBFv3_RS10800 at 2394.401 kb on + strand, within RALBFv3_RS10800 at 2394.401 kb on + strand, within RALBFv3_RS10800 at 2394.402 kb on - strand, within RALBFv3_RS10800 at 2394.452 kb on + strand, within RALBFv3_RS10800 at 2394.452 kb on + strand, within RALBFv3_RS10800 at 2394.452 kb on + strand, within RALBFv3_RS10800 at 2394.452 kb on + strand, within RALBFv3_RS10800 at 2394.453 kb on - strand, within RALBFv3_RS10800 at 2394.766 kb on + strand at 2394.766 kb on + strand at 2394.766 kb on + strand at 2394.767 kb on - strand at 2394.767 kb on - strand at 2394.767 kb on - strand at 2394.855 kb on + strand at 2394.855 kb on + strand at 2394.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,391,834 + RALBFv3_RS10790 0.54 +0.2 2,392,339 + RALBFv3_RS10790 0.79 -0.4 2,392,340 - RALBFv3_RS10790 0.79 +0.8 2,392,405 + RALBFv3_RS10790 0.82 -0.2 2,392,879 + -0.4 2,392,879 + -0.0 2,392,880 - -1.0 2,393,686 + RALBFv3_RS10795 0.69 -0.8 2,394,067 + -1.0 2,394,243 - RALBFv3_RS10800 0.12 +0.1 2,394,374 + RALBFv3_RS10800 0.36 +0.4 2,394,375 - RALBFv3_RS10800 0.36 -0.9 2,394,375 - RALBFv3_RS10800 0.36 -0.4 2,394,375 - RALBFv3_RS10800 0.36 -0.8 2,394,401 + RALBFv3_RS10800 0.41 -0.2 2,394,401 + RALBFv3_RS10800 0.41 -0.2 2,394,401 + RALBFv3_RS10800 0.41 -0.1 2,394,402 - RALBFv3_RS10800 0.41 +0.2 2,394,452 + RALBFv3_RS10800 0.50 -0.6 2,394,452 + RALBFv3_RS10800 0.50 -1.4 2,394,452 + RALBFv3_RS10800 0.50 -0.6 2,394,452 + RALBFv3_RS10800 0.50 -0.4 2,394,453 - RALBFv3_RS10800 0.51 +0.1 2,394,766 + +0.1 2,394,766 + -1.9 2,394,766 + -1.3 2,394,767 - -0.5 2,394,767 - +0.1 2,394,767 - +1.1 2,394,855 + +1.1 2,394,855 + -0.2 2,394,855 + -0.6
Or see this region's nucleotide sequence