Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10645 and RALBFv3_RS10650 are separated by 30 nucleotides RALBFv3_RS10650 and RALBFv3_RS23925 are separated by 72 nucleotides RALBFv3_RS23925 and RALBFv3_RS10655 are separated by 4 nucleotides RALBFv3_RS10655 and RALBFv3_RS10660 are separated by 102 nucleotides
RALBFv3_RS10645: RALBFv3_RS10645 - FAA hydrolase family protein, at 2,362,768 to 2,363,760
_RS10645
RALBFv3_RS10650: RALBFv3_RS10650 - IclR family transcriptional regulator, at 2,363,791 to 2,364,687
_RS10650
RALBFv3_RS23925: RALBFv3_RS23925 - hypothetical protein, at 2,364,760 to 2,364,981
_RS23925
RALBFv3_RS10655: RALBFv3_RS10655 - hypothetical protein, at 2,364,986 to 2,365,186
_RS10655
RALBFv3_RS10660: RALBFv3_RS10660 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,365,289 to 2,366,467
_RS10660
Position (kb)
2363
2364
2365 Strain fitness (log2 ratio)
-2
-1
0
1 at 2362.924 kb on + strand, within RALBFv3_RS10645 at 2363.029 kb on + strand, within RALBFv3_RS10645 at 2363.030 kb on - strand, within RALBFv3_RS10645 at 2363.108 kb on - strand, within RALBFv3_RS10645 at 2363.662 kb on + strand at 2363.793 kb on - strand at 2364.325 kb on + strand, within RALBFv3_RS10650 at 2364.325 kb on + strand, within RALBFv3_RS10650 at 2364.326 kb on - strand, within RALBFv3_RS10650 at 2364.712 kb on + strand at 2364.713 kb on - strand at 2364.713 kb on - strand at 2364.847 kb on + strand, within RALBFv3_RS23925 at 2364.919 kb on + strand, within RALBFv3_RS23925 at 2364.920 kb on - strand, within RALBFv3_RS23925 at 2364.920 kb on - strand, within RALBFv3_RS23925 at 2364.977 kb on + strand at 2364.978 kb on - strand at 2365.600 kb on + strand, within RALBFv3_RS10660
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,362,924 + RALBFv3_RS10645 0.16 -0.4 2,363,029 + RALBFv3_RS10645 0.26 +0.5 2,363,030 - RALBFv3_RS10645 0.26 -0.3 2,363,108 - RALBFv3_RS10645 0.34 -0.2 2,363,662 + -1.0 2,363,793 - +0.2 2,364,325 + RALBFv3_RS10650 0.60 -0.8 2,364,325 + RALBFv3_RS10650 0.60 +0.1 2,364,326 - RALBFv3_RS10650 0.60 -1.8 2,364,712 + -0.5 2,364,713 - -0.8 2,364,713 - +0.1 2,364,847 + RALBFv3_RS23925 0.39 -0.3 2,364,919 + RALBFv3_RS23925 0.72 +0.1 2,364,920 - RALBFv3_RS23925 0.72 -0.0 2,364,920 - RALBFv3_RS23925 0.72 +0.1 2,364,977 + +0.6 2,364,978 - +0.7 2,365,600 + RALBFv3_RS10660 0.26 -0.1
Or see this region's nucleotide sequence