Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10650

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRALBFv3_RS10645 and RALBFv3_RS10650 are separated by 30 nucleotidesRALBFv3_RS10650 and RALBFv3_RS23925 are separated by 72 nucleotidesRALBFv3_RS23925 and RALBFv3_RS10655 are separated by 4 nucleotidesRALBFv3_RS10655 and RALBFv3_RS10660 are separated by 102 nucleotides RALBFv3_RS10645: RALBFv3_RS10645 - FAA hydrolase family protein, at 2,362,768 to 2,363,760 _RS10645 RALBFv3_RS10650: RALBFv3_RS10650 - IclR family transcriptional regulator, at 2,363,791 to 2,364,687 _RS10650 RALBFv3_RS23925: RALBFv3_RS23925 - hypothetical protein, at 2,364,760 to 2,364,981 _RS23925 RALBFv3_RS10655: RALBFv3_RS10655 - hypothetical protein, at 2,364,986 to 2,365,186 _RS10655 RALBFv3_RS10660: RALBFv3_RS10660 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,365,289 to 2,366,467 _RS10660 Position (kb) 2363 2364 2365Strain fitness (log2 ratio) -2 -1 0 1at 2362.924 kb on + strand, within RALBFv3_RS10645at 2363.029 kb on + strand, within RALBFv3_RS10645at 2363.030 kb on - strand, within RALBFv3_RS10645at 2363.108 kb on - strand, within RALBFv3_RS10645at 2363.662 kb on + strandat 2363.793 kb on - strandat 2364.325 kb on + strand, within RALBFv3_RS10650at 2364.325 kb on + strand, within RALBFv3_RS10650at 2364.326 kb on - strand, within RALBFv3_RS10650at 2364.712 kb on + strandat 2364.713 kb on - strandat 2364.713 kb on - strandat 2364.847 kb on + strand, within RALBFv3_RS23925at 2364.919 kb on + strand, within RALBFv3_RS23925at 2364.920 kb on - strand, within RALBFv3_RS23925at 2364.920 kb on - strand, within RALBFv3_RS23925at 2364.977 kb on + strandat 2364.978 kb on - strandat 2365.600 kb on + strand, within RALBFv3_RS10660

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
remove
2,362,924 + RALBFv3_RS10645 0.16 -0.4
2,363,029 + RALBFv3_RS10645 0.26 +0.5
2,363,030 - RALBFv3_RS10645 0.26 -0.3
2,363,108 - RALBFv3_RS10645 0.34 -0.2
2,363,662 + -1.0
2,363,793 - +0.2
2,364,325 + RALBFv3_RS10650 0.60 -0.8
2,364,325 + RALBFv3_RS10650 0.60 +0.1
2,364,326 - RALBFv3_RS10650 0.60 -1.8
2,364,712 + -0.5
2,364,713 - -0.8
2,364,713 - +0.1
2,364,847 + RALBFv3_RS23925 0.39 -0.3
2,364,919 + RALBFv3_RS23925 0.72 +0.1
2,364,920 - RALBFv3_RS23925 0.72 -0.0
2,364,920 - RALBFv3_RS23925 0.72 +0.1
2,364,977 + +0.6
2,364,978 - +0.7
2,365,600 + RALBFv3_RS10660 0.26 -0.1

Or see this region's nucleotide sequence