Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10630 and RALBFv3_RS10635 overlap by 4 nucleotides RALBFv3_RS10635 and RALBFv3_RS10640 overlap by 35 nucleotides RALBFv3_RS10640 and RALBFv3_RS10645 are separated by 84 nucleotides
RALBFv3_RS10630: RALBFv3_RS10630 - 3-oxoadipate enol-lactonase, at 2,359,864 to 2,360,703
_RS10630
RALBFv3_RS10635: RALBFv3_RS10635 - DUF3108 domain-containing protein, at 2,360,700 to 2,361,848
_RS10635
RALBFv3_RS10640: RALBFv3_RS10640 - IclR family transcriptional regulator, at 2,361,814 to 2,362,683
_RS10640
RALBFv3_RS10645: RALBFv3_RS10645 - FAA hydrolase family protein, at 2,362,768 to 2,363,760
_RS10645
Position (kb)
2360
2361
2362 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2359.750 kb on + strand at 2359.750 kb on + strand at 2360.313 kb on + strand, within RALBFv3_RS10630 at 2360.313 kb on + strand, within RALBFv3_RS10630 at 2360.526 kb on - strand, within RALBFv3_RS10630 at 2360.526 kb on - strand, within RALBFv3_RS10630 at 2360.526 kb on - strand, within RALBFv3_RS10630 at 2360.740 kb on + strand at 2360.740 kb on + strand at 2360.741 kb on - strand at 2361.296 kb on - strand, within RALBFv3_RS10635 at 2361.355 kb on + strand, within RALBFv3_RS10635 at 2361.355 kb on + strand, within RALBFv3_RS10635 at 2361.355 kb on + strand, within RALBFv3_RS10635 at 2361.356 kb on - strand, within RALBFv3_RS10635 at 2361.364 kb on + strand, within RALBFv3_RS10635 at 2361.365 kb on - strand, within RALBFv3_RS10635 at 2361.765 kb on + strand at 2361.765 kb on + strand at 2361.766 kb on - strand at 2361.843 kb on - strand at 2361.868 kb on + strand at 2361.868 kb on + strand at 2361.869 kb on - strand at 2361.869 kb on - strand at 2361.869 kb on - strand at 2362.068 kb on - strand, within RALBFv3_RS10640 at 2362.187 kb on - strand, within RALBFv3_RS10640 at 2362.732 kb on - strand at 2362.732 kb on - strand at 2362.732 kb on - strand at 2362.732 kb on - strand at 2362.732 kb on - strand at 2362.732 kb on - strand at 2362.732 kb on - strand at 2362.761 kb on + strand at 2362.761 kb on + strand at 2362.761 kb on + strand at 2362.762 kb on - strand at 2362.762 kb on - strand at 2362.766 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,359,750 + -0.2 2,359,750 + +0.7 2,360,313 + RALBFv3_RS10630 0.53 -0.0 2,360,313 + RALBFv3_RS10630 0.53 -0.1 2,360,526 - RALBFv3_RS10630 0.79 +0.5 2,360,526 - RALBFv3_RS10630 0.79 +2.7 2,360,526 - RALBFv3_RS10630 0.79 -1.0 2,360,740 + +0.1 2,360,740 + -1.9 2,360,741 - -1.7 2,361,296 - RALBFv3_RS10635 0.52 -0.8 2,361,355 + RALBFv3_RS10635 0.57 -0.8 2,361,355 + RALBFv3_RS10635 0.57 -2.5 2,361,355 + RALBFv3_RS10635 0.57 +1.1 2,361,356 - RALBFv3_RS10635 0.57 -0.1 2,361,364 + RALBFv3_RS10635 0.58 -1.2 2,361,365 - RALBFv3_RS10635 0.58 +0.1 2,361,765 + -0.4 2,361,765 + -0.3 2,361,766 - -0.8 2,361,843 - -0.5 2,361,868 + -0.1 2,361,868 + -0.6 2,361,869 - -0.0 2,361,869 - +1.1 2,361,869 - +0.5 2,362,068 - RALBFv3_RS10640 0.29 +0.5 2,362,187 - RALBFv3_RS10640 0.43 +1.1 2,362,732 - -0.8 2,362,732 - -0.1 2,362,732 - +0.3 2,362,732 - +0.1 2,362,732 - +0.1 2,362,732 - +1.1 2,362,732 - -1.5 2,362,761 + -0.1 2,362,761 + -0.0 2,362,761 + -0.0 2,362,762 - +0.4 2,362,762 - +0.1 2,362,766 - -0.4
Or see this region's nucleotide sequence