Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10575 and RALBFv3_RS10580 are separated by 204 nucleotides RALBFv3_RS10580 and RALBFv3_RS10585 are separated by 142 nucleotides RALBFv3_RS10585 and RALBFv3_RS10590 are separated by 73 nucleotides RALBFv3_RS10590 and RALBFv3_RS10595 are separated by 70 nucleotides RALBFv3_RS10595 and RALBFv3_RS10600 are separated by 114 nucleotides
RALBFv3_RS10575: RALBFv3_RS10575 - DUF1840 domain-containing protein, at 2,350,093 to 2,350,413
_RS10575
RALBFv3_RS10580: RALBFv3_RS10580 - DUF1328 domain-containing protein, at 2,350,618 to 2,350,815
_RS10580
RALBFv3_RS10585: RALBFv3_RS10585 - sensor histidine kinase, at 2,350,958 to 2,352,385
_RS10585
RALBFv3_RS10590: RALBFv3_RS10590 - entericidin, EcnA/B family, at 2,352,459 to 2,352,599
_RS10590
RALBFv3_RS10595: RALBFv3_RS10595 - response regulator, at 2,352,670 to 2,353,110
_RS10595
RALBFv3_RS10600: RALBFv3_RS10600 - DNA-binding response regulator, at 2,353,225 to 2,353,857
_RS10600
Position (kb)
2350
2351
2352
2353 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2350.064 kb on + strand at 2350.064 kb on + strand at 2350.065 kb on - strand at 2350.065 kb on - strand at 2350.104 kb on + strand at 2350.105 kb on - strand at 2350.349 kb on - strand, within RALBFv3_RS10575 at 2350.411 kb on + strand at 2350.875 kb on + strand at 2350.875 kb on + strand at 2350.875 kb on + strand at 2350.876 kb on - strand at 2350.876 kb on - strand at 2350.989 kb on + strand at 2351.019 kb on + strand at 2351.177 kb on + strand, within RALBFv3_RS10585 at 2351.177 kb on + strand, within RALBFv3_RS10585 at 2351.189 kb on + strand, within RALBFv3_RS10585 at 2351.243 kb on + strand, within RALBFv3_RS10585 at 2351.333 kb on + strand, within RALBFv3_RS10585 at 2351.333 kb on + strand, within RALBFv3_RS10585 at 2351.333 kb on + strand, within RALBFv3_RS10585 at 2351.334 kb on - strand, within RALBFv3_RS10585 at 2352.029 kb on + strand, within RALBFv3_RS10585 at 2352.069 kb on - strand, within RALBFv3_RS10585 at 2352.069 kb on - strand, within RALBFv3_RS10585 at 2352.439 kb on + strand at 2352.460 kb on + strand at 2352.460 kb on + strand at 2352.461 kb on - strand at 2352.461 kb on - strand at 2352.777 kb on - strand, within RALBFv3_RS10595 at 2352.777 kb on - strand, within RALBFv3_RS10595 at 2352.837 kb on - strand, within RALBFv3_RS10595 at 2353.020 kb on - strand, within RALBFv3_RS10595 at 2353.253 kb on + strand at 2353.253 kb on + strand at 2353.256 kb on + strand at 2353.256 kb on + strand at 2353.256 kb on + strand at 2353.256 kb on + strand at 2353.256 kb on + strand at 2353.256 kb on + strand at 2353.257 kb on - strand at 2353.257 kb on - strand at 2353.257 kb on - strand at 2353.311 kb on - strand, within RALBFv3_RS10600 at 2353.314 kb on + strand, within RALBFv3_RS10600 at 2353.315 kb on - strand, within RALBFv3_RS10600
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,350,064 + +0.1 2,350,064 + +0.4 2,350,065 - -0.2 2,350,065 - -0.1 2,350,104 + +0.5 2,350,105 - +1.1 2,350,349 - RALBFv3_RS10575 0.80 -0.6 2,350,411 + +0.8 2,350,875 + +0.2 2,350,875 + -0.0 2,350,875 + +0.7 2,350,876 - +0.2 2,350,876 - -0.5 2,350,989 + -2.0 2,351,019 + -2.1 2,351,177 + RALBFv3_RS10585 0.15 -2.2 2,351,177 + RALBFv3_RS10585 0.15 -1.8 2,351,189 + RALBFv3_RS10585 0.16 -1.7 2,351,243 + RALBFv3_RS10585 0.20 +1.1 2,351,333 + RALBFv3_RS10585 0.26 -2.3 2,351,333 + RALBFv3_RS10585 0.26 -2.2 2,351,333 + RALBFv3_RS10585 0.26 -2.4 2,351,334 - RALBFv3_RS10585 0.26 -2.4 2,352,029 + RALBFv3_RS10585 0.75 -1.2 2,352,069 - RALBFv3_RS10585 0.78 -2.2 2,352,069 - RALBFv3_RS10585 0.78 -2.2 2,352,439 + -0.1 2,352,460 + -0.1 2,352,460 + -0.6 2,352,461 - -0.5 2,352,461 - +0.7 2,352,777 - RALBFv3_RS10595 0.24 +1.3 2,352,777 - RALBFv3_RS10595 0.24 -0.1 2,352,837 - RALBFv3_RS10595 0.38 +0.9 2,353,020 - RALBFv3_RS10595 0.79 -0.5 2,353,253 + -1.9 2,353,253 + -1.8 2,353,256 + -1.5 2,353,256 + -2.3 2,353,256 + -2.2 2,353,256 + -1.9 2,353,256 + -2.8 2,353,256 + -2.2 2,353,257 - -1.6 2,353,257 - -2.1 2,353,257 - +1.1 2,353,311 - RALBFv3_RS10600 0.14 +0.1 2,353,314 + RALBFv3_RS10600 0.14 -2.0 2,353,315 - RALBFv3_RS10600 0.14 -1.8
Or see this region's nucleotide sequence