Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10565

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10555 and RALBFv3_RS10560 are separated by 113 nucleotidesRALBFv3_RS10560 and RALBFv3_RS10565 are separated by 101 nucleotidesRALBFv3_RS10565 and RALBFv3_RS10570 are separated by 58 nucleotides RALBFv3_RS10555: RALBFv3_RS10555 - short-chain dehydrogenase, at 2,345,657 to 2,346,457 _RS10555 RALBFv3_RS10560: RALBFv3_RS10560 - thiol:disulfide interchange protein DsbA/DsbL, at 2,346,571 to 2,347,227 _RS10560 RALBFv3_RS10565: RALBFv3_RS10565 - SPOR domain-containing protein, at 2,347,329 to 2,348,051 _RS10565 RALBFv3_RS10570: RALBFv3_RS10570 - arginine--tRNA ligase, at 2,348,110 to 2,349,912 _RS10570 Position (kb) 2347 2348 2349Strain fitness (log2 ratio) -2 -1 0 1at 2346.663 kb on - strand, within RALBFv3_RS10560at 2346.762 kb on - strand, within RALBFv3_RS10560at 2346.935 kb on + strand, within RALBFv3_RS10560at 2346.935 kb on + strand, within RALBFv3_RS10560at 2347.439 kb on - strand, within RALBFv3_RS10565at 2347.537 kb on + strand, within RALBFv3_RS10565at 2347.537 kb on + strand, within RALBFv3_RS10565at 2347.940 kb on - strand, within RALBFv3_RS10565at 2347.940 kb on - strand, within RALBFv3_RS10565at 2348.063 kb on + strandat 2348.079 kb on - strandat 2348.112 kb on - strandat 2348.112 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,346,663 - RALBFv3_RS10560 0.14 +0.7
2,346,762 - RALBFv3_RS10560 0.29 +1.1
2,346,935 + RALBFv3_RS10560 0.55 +0.7
2,346,935 + RALBFv3_RS10560 0.55 +0.1
2,347,439 - RALBFv3_RS10565 0.15 -0.7
2,347,537 + RALBFv3_RS10565 0.29 -0.9
2,347,537 + RALBFv3_RS10565 0.29 -1.2
2,347,940 - RALBFv3_RS10565 0.85 -1.1
2,347,940 - RALBFv3_RS10565 0.85 -2.0
2,348,063 + -1.2
2,348,079 - +1.6
2,348,112 - +0.2
2,348,112 - +0.2

Or see this region's nucleotide sequence