Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10230

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10220 and RALBFv3_RS10225 are separated by 179 nucleotidesRALBFv3_RS10225 and RALBFv3_RS10230 overlap by 4 nucleotidesRALBFv3_RS10230 and RALBFv3_RS10235 are separated by 3 nucleotides RALBFv3_RS10220: RALBFv3_RS10220 - NAD-dependent epimerase, at 2,271,343 to 2,272,281 _RS10220 RALBFv3_RS10225: RALBFv3_RS10225 - short-chain dehydrogenase, at 2,272,461 to 2,273,201 _RS10225 RALBFv3_RS10230: RALBFv3_RS10230 - FAD-binding oxidoreductase, at 2,273,198 to 2,274,514 _RS10230 RALBFv3_RS10235: RALBFv3_RS10235 - membrane protein, at 2,274,518 to 2,275,942 _RS10235 Position (kb) 2273 2274 2275Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2272.462 kb on + strandat 2272.462 kb on + strandat 2272.540 kb on + strand, within RALBFv3_RS10225at 2272.541 kb on - strand, within RALBFv3_RS10225at 2272.750 kb on + strand, within RALBFv3_RS10225at 2272.756 kb on + strand, within RALBFv3_RS10225at 2272.757 kb on - strand, within RALBFv3_RS10225at 2272.757 kb on - strand, within RALBFv3_RS10225at 2273.325 kb on + strandat 2273.326 kb on - strandat 2273.598 kb on + strand, within RALBFv3_RS10230at 2273.640 kb on + strand, within RALBFv3_RS10230at 2273.649 kb on + strand, within RALBFv3_RS10230at 2273.649 kb on + strand, within RALBFv3_RS10230at 2273.650 kb on - strand, within RALBFv3_RS10230at 2273.694 kb on + strand, within RALBFv3_RS10230at 2273.696 kb on - strandat 2273.877 kb on + strand, within RALBFv3_RS10230at 2274.190 kb on - strand, within RALBFv3_RS10230at 2274.190 kb on - strand, within RALBFv3_RS10230at 2274.369 kb on + strand, within RALBFv3_RS10230at 2274.652 kb on - strandat 2274.652 kb on - strandat 2274.726 kb on + strand, within RALBFv3_RS10235at 2274.726 kb on + strand, within RALBFv3_RS10235at 2274.727 kb on - strand, within RALBFv3_RS10235at 2274.727 kb on - strand, within RALBFv3_RS10235at 2274.753 kb on + strand, within RALBFv3_RS10235at 2274.804 kb on + strand, within RALBFv3_RS10235at 2274.861 kb on + strand, within RALBFv3_RS10235at 2274.969 kb on + strand, within RALBFv3_RS10235at 2274.969 kb on + strand, within RALBFv3_RS10235at 2275.045 kb on - strand, within RALBFv3_RS10235at 2275.047 kb on + strand, within RALBFv3_RS10235at 2275.233 kb on + strand, within RALBFv3_RS10235at 2275.233 kb on + strand, within RALBFv3_RS10235at 2275.234 kb on - strand, within RALBFv3_RS10235at 2275.234 kb on - strand, within RALBFv3_RS10235at 2275.234 kb on - strand, within RALBFv3_RS10235at 2275.234 kb on - strand, within RALBFv3_RS10235at 2275.476 kb on + strand, within RALBFv3_RS10235at 2275.476 kb on + strand, within RALBFv3_RS10235at 2275.477 kb on - strand, within RALBFv3_RS10235at 2275.477 kb on - strand, within RALBFv3_RS10235

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,272,462 + -0.9
2,272,462 + -1.9
2,272,540 + RALBFv3_RS10225 0.11 -0.5
2,272,541 - RALBFv3_RS10225 0.11 +1.1
2,272,750 + RALBFv3_RS10225 0.39 -0.9
2,272,756 + RALBFv3_RS10225 0.40 -0.5
2,272,757 - RALBFv3_RS10225 0.40 -0.5
2,272,757 - RALBFv3_RS10225 0.40 -1.6
2,273,325 + -2.2
2,273,326 - -2.3
2,273,598 + RALBFv3_RS10230 0.30 -2.6
2,273,640 + RALBFv3_RS10230 0.34 -1.2
2,273,649 + RALBFv3_RS10230 0.34 -2.1
2,273,649 + RALBFv3_RS10230 0.34 -0.5
2,273,650 - RALBFv3_RS10230 0.34 -1.4
2,273,694 + RALBFv3_RS10230 0.38 -1.4
2,273,696 - +0.0
2,273,877 + RALBFv3_RS10230 0.52 -0.6
2,274,190 - RALBFv3_RS10230 0.75 -3.0
2,274,190 - RALBFv3_RS10230 0.75 -2.3
2,274,369 + RALBFv3_RS10230 0.89 -0.8
2,274,652 - -0.5
2,274,652 - +0.1
2,274,726 + RALBFv3_RS10235 0.15 -1.1
2,274,726 + RALBFv3_RS10235 0.15 +0.2
2,274,727 - RALBFv3_RS10235 0.15 -0.1
2,274,727 - RALBFv3_RS10235 0.15 -0.1
2,274,753 + RALBFv3_RS10235 0.16 +1.0
2,274,804 + RALBFv3_RS10235 0.20 -0.0
2,274,861 + RALBFv3_RS10235 0.24 -0.5
2,274,969 + RALBFv3_RS10235 0.32 +1.0
2,274,969 + RALBFv3_RS10235 0.32 -0.3
2,275,045 - RALBFv3_RS10235 0.37 -0.1
2,275,047 + RALBFv3_RS10235 0.37 -0.5
2,275,233 + RALBFv3_RS10235 0.50 -1.0
2,275,233 + RALBFv3_RS10235 0.50 -1.2
2,275,234 - RALBFv3_RS10235 0.50 -0.1
2,275,234 - RALBFv3_RS10235 0.50 -0.4
2,275,234 - RALBFv3_RS10235 0.50 +0.3
2,275,234 - RALBFv3_RS10235 0.50 +0.0
2,275,476 + RALBFv3_RS10235 0.67 +0.5
2,275,476 + RALBFv3_RS10235 0.67 +2.6
2,275,477 - RALBFv3_RS10235 0.67 -0.5
2,275,477 - RALBFv3_RS10235 0.67 -0.1

Or see this region's nucleotide sequence