Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10220 and RALBFv3_RS10225 are separated by 179 nucleotides RALBFv3_RS10225 and RALBFv3_RS10230 overlap by 4 nucleotides RALBFv3_RS10230 and RALBFv3_RS10235 are separated by 3 nucleotides
RALBFv3_RS10220: RALBFv3_RS10220 - NAD-dependent epimerase, at 2,271,343 to 2,272,281
_RS10220
RALBFv3_RS10225: RALBFv3_RS10225 - short-chain dehydrogenase, at 2,272,461 to 2,273,201
_RS10225
RALBFv3_RS10230: RALBFv3_RS10230 - FAD-binding oxidoreductase, at 2,273,198 to 2,274,514
_RS10230
RALBFv3_RS10235: RALBFv3_RS10235 - membrane protein, at 2,274,518 to 2,275,942
_RS10235
Position (kb)
2273
2274
2275 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2272.462 kb on + strand at 2272.462 kb on + strand at 2272.540 kb on + strand, within RALBFv3_RS10225 at 2272.541 kb on - strand, within RALBFv3_RS10225 at 2272.750 kb on + strand, within RALBFv3_RS10225 at 2272.756 kb on + strand, within RALBFv3_RS10225 at 2272.757 kb on - strand, within RALBFv3_RS10225 at 2272.757 kb on - strand, within RALBFv3_RS10225 at 2273.325 kb on + strand at 2273.326 kb on - strand at 2273.598 kb on + strand, within RALBFv3_RS10230 at 2273.640 kb on + strand, within RALBFv3_RS10230 at 2273.649 kb on + strand, within RALBFv3_RS10230 at 2273.649 kb on + strand, within RALBFv3_RS10230 at 2273.650 kb on - strand, within RALBFv3_RS10230 at 2273.694 kb on + strand, within RALBFv3_RS10230 at 2273.696 kb on - strand at 2273.877 kb on + strand, within RALBFv3_RS10230 at 2274.190 kb on - strand, within RALBFv3_RS10230 at 2274.190 kb on - strand, within RALBFv3_RS10230 at 2274.369 kb on + strand, within RALBFv3_RS10230 at 2274.652 kb on - strand at 2274.652 kb on - strand at 2274.726 kb on + strand, within RALBFv3_RS10235 at 2274.726 kb on + strand, within RALBFv3_RS10235 at 2274.727 kb on - strand, within RALBFv3_RS10235 at 2274.727 kb on - strand, within RALBFv3_RS10235 at 2274.753 kb on + strand, within RALBFv3_RS10235 at 2274.804 kb on + strand, within RALBFv3_RS10235 at 2274.861 kb on + strand, within RALBFv3_RS10235 at 2274.969 kb on + strand, within RALBFv3_RS10235 at 2274.969 kb on + strand, within RALBFv3_RS10235 at 2275.045 kb on - strand, within RALBFv3_RS10235 at 2275.047 kb on + strand, within RALBFv3_RS10235 at 2275.233 kb on + strand, within RALBFv3_RS10235 at 2275.233 kb on + strand, within RALBFv3_RS10235 at 2275.234 kb on - strand, within RALBFv3_RS10235 at 2275.234 kb on - strand, within RALBFv3_RS10235 at 2275.234 kb on - strand, within RALBFv3_RS10235 at 2275.234 kb on - strand, within RALBFv3_RS10235 at 2275.476 kb on + strand, within RALBFv3_RS10235 at 2275.476 kb on + strand, within RALBFv3_RS10235 at 2275.477 kb on - strand, within RALBFv3_RS10235 at 2275.477 kb on - strand, within RALBFv3_RS10235
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,272,462 + -0.9 2,272,462 + -1.9 2,272,540 + RALBFv3_RS10225 0.11 -0.5 2,272,541 - RALBFv3_RS10225 0.11 +1.1 2,272,750 + RALBFv3_RS10225 0.39 -0.9 2,272,756 + RALBFv3_RS10225 0.40 -0.5 2,272,757 - RALBFv3_RS10225 0.40 -0.5 2,272,757 - RALBFv3_RS10225 0.40 -1.6 2,273,325 + -2.2 2,273,326 - -2.3 2,273,598 + RALBFv3_RS10230 0.30 -2.6 2,273,640 + RALBFv3_RS10230 0.34 -1.2 2,273,649 + RALBFv3_RS10230 0.34 -2.1 2,273,649 + RALBFv3_RS10230 0.34 -0.5 2,273,650 - RALBFv3_RS10230 0.34 -1.4 2,273,694 + RALBFv3_RS10230 0.38 -1.4 2,273,696 - +0.0 2,273,877 + RALBFv3_RS10230 0.52 -0.6 2,274,190 - RALBFv3_RS10230 0.75 -3.0 2,274,190 - RALBFv3_RS10230 0.75 -2.3 2,274,369 + RALBFv3_RS10230 0.89 -0.8 2,274,652 - -0.5 2,274,652 - +0.1 2,274,726 + RALBFv3_RS10235 0.15 -1.1 2,274,726 + RALBFv3_RS10235 0.15 +0.2 2,274,727 - RALBFv3_RS10235 0.15 -0.1 2,274,727 - RALBFv3_RS10235 0.15 -0.1 2,274,753 + RALBFv3_RS10235 0.16 +1.0 2,274,804 + RALBFv3_RS10235 0.20 -0.0 2,274,861 + RALBFv3_RS10235 0.24 -0.5 2,274,969 + RALBFv3_RS10235 0.32 +1.0 2,274,969 + RALBFv3_RS10235 0.32 -0.3 2,275,045 - RALBFv3_RS10235 0.37 -0.1 2,275,047 + RALBFv3_RS10235 0.37 -0.5 2,275,233 + RALBFv3_RS10235 0.50 -1.0 2,275,233 + RALBFv3_RS10235 0.50 -1.2 2,275,234 - RALBFv3_RS10235 0.50 -0.1 2,275,234 - RALBFv3_RS10235 0.50 -0.4 2,275,234 - RALBFv3_RS10235 0.50 +0.3 2,275,234 - RALBFv3_RS10235 0.50 +0.0 2,275,476 + RALBFv3_RS10235 0.67 +0.5 2,275,476 + RALBFv3_RS10235 0.67 +2.6 2,275,477 - RALBFv3_RS10235 0.67 -0.5 2,275,477 - RALBFv3_RS10235 0.67 -0.1
Or see this region's nucleotide sequence