Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10220 and RALBFv3_RS10225 are separated by 179 nucleotides RALBFv3_RS10225 and RALBFv3_RS10230 overlap by 4 nucleotides
RALBFv3_RS10220: RALBFv3_RS10220 - NAD-dependent epimerase, at 2,271,343 to 2,272,281
_RS10220
RALBFv3_RS10225: RALBFv3_RS10225 - short-chain dehydrogenase, at 2,272,461 to 2,273,201
_RS10225
RALBFv3_RS10230: RALBFv3_RS10230 - FAD-binding oxidoreductase, at 2,273,198 to 2,274,514
_RS10230
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2271.524 kb on + strand, within RALBFv3_RS10220 at 2271.524 kb on + strand, within RALBFv3_RS10220 at 2271.525 kb on - strand, within RALBFv3_RS10220 at 2271.542 kb on + strand, within RALBFv3_RS10220 at 2271.542 kb on + strand, within RALBFv3_RS10220 at 2271.542 kb on + strand, within RALBFv3_RS10220 at 2271.543 kb on - strand, within RALBFv3_RS10220 at 2271.543 kb on - strand, within RALBFv3_RS10220 at 2271.543 kb on - strand, within RALBFv3_RS10220 at 2271.620 kb on + strand, within RALBFv3_RS10220 at 2271.621 kb on - strand, within RALBFv3_RS10220 at 2271.621 kb on - strand, within RALBFv3_RS10220 at 2271.713 kb on + strand, within RALBFv3_RS10220 at 2271.732 kb on - strand, within RALBFv3_RS10220 at 2271.899 kb on + strand, within RALBFv3_RS10220 at 2271.939 kb on + strand, within RALBFv3_RS10220 at 2271.939 kb on + strand, within RALBFv3_RS10220 at 2271.950 kb on + strand, within RALBFv3_RS10220 at 2271.962 kb on + strand, within RALBFv3_RS10220 at 2272.197 kb on + strand at 2272.462 kb on + strand at 2272.462 kb on + strand at 2272.540 kb on + strand, within RALBFv3_RS10225 at 2272.541 kb on - strand, within RALBFv3_RS10225 at 2272.750 kb on + strand, within RALBFv3_RS10225 at 2272.756 kb on + strand, within RALBFv3_RS10225 at 2272.757 kb on - strand, within RALBFv3_RS10225 at 2272.757 kb on - strand, within RALBFv3_RS10225 at 2273.325 kb on + strand at 2273.326 kb on - strand at 2273.598 kb on + strand, within RALBFv3_RS10230 at 2273.640 kb on + strand, within RALBFv3_RS10230 at 2273.649 kb on + strand, within RALBFv3_RS10230 at 2273.649 kb on + strand, within RALBFv3_RS10230 at 2273.650 kb on - strand, within RALBFv3_RS10230 at 2273.694 kb on + strand, within RALBFv3_RS10230 at 2273.696 kb on - strand at 2273.877 kb on + strand, within RALBFv3_RS10230 at 2274.190 kb on - strand, within RALBFv3_RS10230 at 2274.190 kb on - strand, within RALBFv3_RS10230
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,271,524 + RALBFv3_RS10220 0.19 +0.5 2,271,524 + RALBFv3_RS10220 0.19 -0.8 2,271,525 - RALBFv3_RS10220 0.19 -1.9 2,271,542 + RALBFv3_RS10220 0.21 +1.1 2,271,542 + RALBFv3_RS10220 0.21 -1.3 2,271,542 + RALBFv3_RS10220 0.21 -2.3 2,271,543 - RALBFv3_RS10220 0.21 -1.9 2,271,543 - RALBFv3_RS10220 0.21 -0.3 2,271,543 - RALBFv3_RS10220 0.21 +1.1 2,271,620 + RALBFv3_RS10220 0.29 -1.7 2,271,621 - RALBFv3_RS10220 0.30 +0.1 2,271,621 - RALBFv3_RS10220 0.30 -2.4 2,271,713 + RALBFv3_RS10220 0.39 -1.8 2,271,732 - RALBFv3_RS10220 0.41 -0.9 2,271,899 + RALBFv3_RS10220 0.59 -2.4 2,271,939 + RALBFv3_RS10220 0.63 -0.9 2,271,939 + RALBFv3_RS10220 0.63 -1.8 2,271,950 + RALBFv3_RS10220 0.65 -2.2 2,271,962 + RALBFv3_RS10220 0.66 -3.3 2,272,197 + -0.5 2,272,462 + -0.9 2,272,462 + -1.9 2,272,540 + RALBFv3_RS10225 0.11 -0.5 2,272,541 - RALBFv3_RS10225 0.11 +1.1 2,272,750 + RALBFv3_RS10225 0.39 -0.9 2,272,756 + RALBFv3_RS10225 0.40 -0.5 2,272,757 - RALBFv3_RS10225 0.40 -0.5 2,272,757 - RALBFv3_RS10225 0.40 -1.6 2,273,325 + -2.2 2,273,326 - -2.3 2,273,598 + RALBFv3_RS10230 0.30 -2.6 2,273,640 + RALBFv3_RS10230 0.34 -1.2 2,273,649 + RALBFv3_RS10230 0.34 -2.1 2,273,649 + RALBFv3_RS10230 0.34 -0.5 2,273,650 - RALBFv3_RS10230 0.34 -1.4 2,273,694 + RALBFv3_RS10230 0.38 -1.4 2,273,696 - +0.0 2,273,877 + RALBFv3_RS10230 0.52 -0.6 2,274,190 - RALBFv3_RS10230 0.75 -3.0 2,274,190 - RALBFv3_RS10230 0.75 -2.3
Or see this region's nucleotide sequence