Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10225

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10220 and RALBFv3_RS10225 are separated by 179 nucleotidesRALBFv3_RS10225 and RALBFv3_RS10230 overlap by 4 nucleotides RALBFv3_RS10220: RALBFv3_RS10220 - NAD-dependent epimerase, at 2,271,343 to 2,272,281 _RS10220 RALBFv3_RS10225: RALBFv3_RS10225 - short-chain dehydrogenase, at 2,272,461 to 2,273,201 _RS10225 RALBFv3_RS10230: RALBFv3_RS10230 - FAD-binding oxidoreductase, at 2,273,198 to 2,274,514 _RS10230 Position (kb) 2272 2273 2274Strain fitness (log2 ratio) -3 -2 -1 0 1at 2271.524 kb on + strand, within RALBFv3_RS10220at 2271.524 kb on + strand, within RALBFv3_RS10220at 2271.525 kb on - strand, within RALBFv3_RS10220at 2271.542 kb on + strand, within RALBFv3_RS10220at 2271.542 kb on + strand, within RALBFv3_RS10220at 2271.542 kb on + strand, within RALBFv3_RS10220at 2271.543 kb on - strand, within RALBFv3_RS10220at 2271.543 kb on - strand, within RALBFv3_RS10220at 2271.543 kb on - strand, within RALBFv3_RS10220at 2271.620 kb on + strand, within RALBFv3_RS10220at 2271.621 kb on - strand, within RALBFv3_RS10220at 2271.621 kb on - strand, within RALBFv3_RS10220at 2271.713 kb on + strand, within RALBFv3_RS10220at 2271.732 kb on - strand, within RALBFv3_RS10220at 2271.899 kb on + strand, within RALBFv3_RS10220at 2271.939 kb on + strand, within RALBFv3_RS10220at 2271.939 kb on + strand, within RALBFv3_RS10220at 2271.950 kb on + strand, within RALBFv3_RS10220at 2271.962 kb on + strand, within RALBFv3_RS10220at 2272.197 kb on + strandat 2272.462 kb on + strandat 2272.462 kb on + strandat 2272.540 kb on + strand, within RALBFv3_RS10225at 2272.541 kb on - strand, within RALBFv3_RS10225at 2272.750 kb on + strand, within RALBFv3_RS10225at 2272.756 kb on + strand, within RALBFv3_RS10225at 2272.757 kb on - strand, within RALBFv3_RS10225at 2272.757 kb on - strand, within RALBFv3_RS10225at 2273.325 kb on + strandat 2273.326 kb on - strandat 2273.598 kb on + strand, within RALBFv3_RS10230at 2273.640 kb on + strand, within RALBFv3_RS10230at 2273.649 kb on + strand, within RALBFv3_RS10230at 2273.649 kb on + strand, within RALBFv3_RS10230at 2273.650 kb on - strand, within RALBFv3_RS10230at 2273.694 kb on + strand, within RALBFv3_RS10230at 2273.696 kb on - strandat 2273.877 kb on + strand, within RALBFv3_RS10230at 2274.190 kb on - strand, within RALBFv3_RS10230at 2274.190 kb on - strand, within RALBFv3_RS10230

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,271,524 + RALBFv3_RS10220 0.19 +0.5
2,271,524 + RALBFv3_RS10220 0.19 -0.8
2,271,525 - RALBFv3_RS10220 0.19 -1.9
2,271,542 + RALBFv3_RS10220 0.21 +1.1
2,271,542 + RALBFv3_RS10220 0.21 -1.3
2,271,542 + RALBFv3_RS10220 0.21 -2.3
2,271,543 - RALBFv3_RS10220 0.21 -1.9
2,271,543 - RALBFv3_RS10220 0.21 -0.3
2,271,543 - RALBFv3_RS10220 0.21 +1.1
2,271,620 + RALBFv3_RS10220 0.29 -1.7
2,271,621 - RALBFv3_RS10220 0.30 +0.1
2,271,621 - RALBFv3_RS10220 0.30 -2.4
2,271,713 + RALBFv3_RS10220 0.39 -1.8
2,271,732 - RALBFv3_RS10220 0.41 -0.9
2,271,899 + RALBFv3_RS10220 0.59 -2.4
2,271,939 + RALBFv3_RS10220 0.63 -0.9
2,271,939 + RALBFv3_RS10220 0.63 -1.8
2,271,950 + RALBFv3_RS10220 0.65 -2.2
2,271,962 + RALBFv3_RS10220 0.66 -3.3
2,272,197 + -0.5
2,272,462 + -0.9
2,272,462 + -1.9
2,272,540 + RALBFv3_RS10225 0.11 -0.5
2,272,541 - RALBFv3_RS10225 0.11 +1.1
2,272,750 + RALBFv3_RS10225 0.39 -0.9
2,272,756 + RALBFv3_RS10225 0.40 -0.5
2,272,757 - RALBFv3_RS10225 0.40 -0.5
2,272,757 - RALBFv3_RS10225 0.40 -1.6
2,273,325 + -2.2
2,273,326 - -2.3
2,273,598 + RALBFv3_RS10230 0.30 -2.6
2,273,640 + RALBFv3_RS10230 0.34 -1.2
2,273,649 + RALBFv3_RS10230 0.34 -2.1
2,273,649 + RALBFv3_RS10230 0.34 -0.5
2,273,650 - RALBFv3_RS10230 0.34 -1.4
2,273,694 + RALBFv3_RS10230 0.38 -1.4
2,273,696 - +0.0
2,273,877 + RALBFv3_RS10230 0.52 -0.6
2,274,190 - RALBFv3_RS10230 0.75 -3.0
2,274,190 - RALBFv3_RS10230 0.75 -2.3

Or see this region's nucleotide sequence