Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS10205 and RALBFv3_RS10210 are separated by 101 nucleotides RALBFv3_RS10210 and RALBFv3_RS10215 overlap by 4 nucleotides RALBFv3_RS10215 and RALBFv3_RS10220 are separated by 13 nucleotides
RALBFv3_RS10205: RALBFv3_RS10205 - RND transporter, at 2,268,086 to 2,269,522
_RS10205
RALBFv3_RS10210: RALBFv3_RS10210 - GtrA family protein, at 2,269,624 to 2,270,049
_RS10210
RALBFv3_RS10215: RALBFv3_RS10215 - hypothetical protein, at 2,270,046 to 2,271,329
_RS10215
RALBFv3_RS10220: RALBFv3_RS10220 - NAD-dependent epimerase, at 2,271,343 to 2,272,281
_RS10220
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2269.220 kb on + strand, within RALBFv3_RS10205 at 2269.221 kb on - strand, within RALBFv3_RS10205 at 2269.221 kb on - strand, within RALBFv3_RS10205 at 2269.583 kb on + strand at 2269.583 kb on + strand at 2269.583 kb on + strand at 2269.584 kb on - strand at 2269.584 kb on - strand at 2269.584 kb on - strand at 2269.895 kb on + strand, within RALBFv3_RS10210 at 2269.938 kb on - strand, within RALBFv3_RS10210 at 2269.938 kb on - strand, within RALBFv3_RS10210 at 2270.114 kb on - strand at 2270.116 kb on + strand at 2270.116 kb on + strand at 2270.117 kb on - strand at 2270.179 kb on + strand, within RALBFv3_RS10215 at 2270.275 kb on + strand, within RALBFv3_RS10215 at 2270.275 kb on + strand, within RALBFv3_RS10215 at 2270.318 kb on - strand, within RALBFv3_RS10215 at 2270.318 kb on - strand, within RALBFv3_RS10215 at 2270.341 kb on + strand, within RALBFv3_RS10215 at 2270.390 kb on - strand, within RALBFv3_RS10215 at 2270.770 kb on + strand, within RALBFv3_RS10215 at 2270.827 kb on + strand, within RALBFv3_RS10215 at 2270.827 kb on + strand, within RALBFv3_RS10215 at 2270.828 kb on - strand, within RALBFv3_RS10215 at 2270.828 kb on - strand, within RALBFv3_RS10215 at 2270.828 kb on - strand, within RALBFv3_RS10215 at 2270.828 kb on - strand, within RALBFv3_RS10215 at 2270.828 kb on - strand, within RALBFv3_RS10215 at 2270.834 kb on - strand, within RALBFv3_RS10215 at 2271.062 kb on - strand, within RALBFv3_RS10215 at 2271.067 kb on + strand, within RALBFv3_RS10215 at 2271.163 kb on + strand, within RALBFv3_RS10215 at 2271.164 kb on - strand, within RALBFv3_RS10215 at 2271.167 kb on - strand, within RALBFv3_RS10215 at 2271.272 kb on - strand at 2271.368 kb on + strand at 2271.369 kb on - strand at 2271.524 kb on + strand, within RALBFv3_RS10220 at 2271.524 kb on + strand, within RALBFv3_RS10220 at 2271.525 kb on - strand, within RALBFv3_RS10220 at 2271.542 kb on + strand, within RALBFv3_RS10220 at 2271.542 kb on + strand, within RALBFv3_RS10220 at 2271.542 kb on + strand, within RALBFv3_RS10220 at 2271.543 kb on - strand, within RALBFv3_RS10220 at 2271.543 kb on - strand, within RALBFv3_RS10220 at 2271.543 kb on - strand, within RALBFv3_RS10220 at 2271.620 kb on + strand, within RALBFv3_RS10220 at 2271.621 kb on - strand, within RALBFv3_RS10220 at 2271.621 kb on - strand, within RALBFv3_RS10220 at 2271.713 kb on + strand, within RALBFv3_RS10220 at 2271.732 kb on - strand, within RALBFv3_RS10220 at 2271.899 kb on + strand, within RALBFv3_RS10220 at 2271.939 kb on + strand, within RALBFv3_RS10220 at 2271.939 kb on + strand, within RALBFv3_RS10220 at 2271.950 kb on + strand, within RALBFv3_RS10220 at 2271.962 kb on + strand, within RALBFv3_RS10220 at 2272.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,269,220 + RALBFv3_RS10205 0.79 -0.6 2,269,221 - RALBFv3_RS10205 0.79 +0.1 2,269,221 - RALBFv3_RS10205 0.79 -0.1 2,269,583 + -0.3 2,269,583 + +0.8 2,269,583 + -0.3 2,269,584 - -1.0 2,269,584 - +1.0 2,269,584 - +1.0 2,269,895 + RALBFv3_RS10210 0.64 +1.0 2,269,938 - RALBFv3_RS10210 0.74 -1.1 2,269,938 - RALBFv3_RS10210 0.74 -1.0 2,270,114 - -0.6 2,270,116 + +0.2 2,270,116 + -0.7 2,270,117 - -1.4 2,270,179 + RALBFv3_RS10215 0.10 -0.0 2,270,275 + RALBFv3_RS10215 0.18 -1.2 2,270,275 + RALBFv3_RS10215 0.18 +2.1 2,270,318 - RALBFv3_RS10215 0.21 -1.4 2,270,318 - RALBFv3_RS10215 0.21 +0.1 2,270,341 + RALBFv3_RS10215 0.23 +1.1 2,270,390 - RALBFv3_RS10215 0.27 -1.2 2,270,770 + RALBFv3_RS10215 0.56 -0.5 2,270,827 + RALBFv3_RS10215 0.61 -0.5 2,270,827 + RALBFv3_RS10215 0.61 -1.2 2,270,828 - RALBFv3_RS10215 0.61 -1.9 2,270,828 - RALBFv3_RS10215 0.61 -1.1 2,270,828 - RALBFv3_RS10215 0.61 +1.1 2,270,828 - RALBFv3_RS10215 0.61 -3.0 2,270,828 - RALBFv3_RS10215 0.61 -0.3 2,270,834 - RALBFv3_RS10215 0.61 +1.1 2,271,062 - RALBFv3_RS10215 0.79 +1.1 2,271,067 + RALBFv3_RS10215 0.80 +0.1 2,271,163 + RALBFv3_RS10215 0.87 -2.6 2,271,164 - RALBFv3_RS10215 0.87 -1.4 2,271,167 - RALBFv3_RS10215 0.87 -1.1 2,271,272 - -1.2 2,271,368 + -0.7 2,271,369 - -0.8 2,271,524 + RALBFv3_RS10220 0.19 +0.5 2,271,524 + RALBFv3_RS10220 0.19 -0.8 2,271,525 - RALBFv3_RS10220 0.19 -1.9 2,271,542 + RALBFv3_RS10220 0.21 +1.1 2,271,542 + RALBFv3_RS10220 0.21 -1.3 2,271,542 + RALBFv3_RS10220 0.21 -2.3 2,271,543 - RALBFv3_RS10220 0.21 -1.9 2,271,543 - RALBFv3_RS10220 0.21 -0.3 2,271,543 - RALBFv3_RS10220 0.21 +1.1 2,271,620 + RALBFv3_RS10220 0.29 -1.7 2,271,621 - RALBFv3_RS10220 0.30 +0.1 2,271,621 - RALBFv3_RS10220 0.30 -2.4 2,271,713 + RALBFv3_RS10220 0.39 -1.8 2,271,732 - RALBFv3_RS10220 0.41 -0.9 2,271,899 + RALBFv3_RS10220 0.59 -2.4 2,271,939 + RALBFv3_RS10220 0.63 -0.9 2,271,939 + RALBFv3_RS10220 0.63 -1.8 2,271,950 + RALBFv3_RS10220 0.65 -2.2 2,271,962 + RALBFv3_RS10220 0.66 -3.3 2,272,197 + -0.5
Or see this region's nucleotide sequence