Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10215

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10205 and RALBFv3_RS10210 are separated by 101 nucleotidesRALBFv3_RS10210 and RALBFv3_RS10215 overlap by 4 nucleotidesRALBFv3_RS10215 and RALBFv3_RS10220 are separated by 13 nucleotides RALBFv3_RS10205: RALBFv3_RS10205 - RND transporter, at 2,268,086 to 2,269,522 _RS10205 RALBFv3_RS10210: RALBFv3_RS10210 - GtrA family protein, at 2,269,624 to 2,270,049 _RS10210 RALBFv3_RS10215: RALBFv3_RS10215 - hypothetical protein, at 2,270,046 to 2,271,329 _RS10215 RALBFv3_RS10220: RALBFv3_RS10220 - NAD-dependent epimerase, at 2,271,343 to 2,272,281 _RS10220 Position (kb) 2270 2271 2272Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2269.220 kb on + strand, within RALBFv3_RS10205at 2269.221 kb on - strand, within RALBFv3_RS10205at 2269.221 kb on - strand, within RALBFv3_RS10205at 2269.583 kb on + strandat 2269.583 kb on + strandat 2269.583 kb on + strandat 2269.584 kb on - strandat 2269.584 kb on - strandat 2269.584 kb on - strandat 2269.895 kb on + strand, within RALBFv3_RS10210at 2269.938 kb on - strand, within RALBFv3_RS10210at 2269.938 kb on - strand, within RALBFv3_RS10210at 2270.114 kb on - strandat 2270.116 kb on + strandat 2270.116 kb on + strandat 2270.117 kb on - strandat 2270.179 kb on + strand, within RALBFv3_RS10215at 2270.275 kb on + strand, within RALBFv3_RS10215at 2270.275 kb on + strand, within RALBFv3_RS10215at 2270.318 kb on - strand, within RALBFv3_RS10215at 2270.318 kb on - strand, within RALBFv3_RS10215at 2270.341 kb on + strand, within RALBFv3_RS10215at 2270.390 kb on - strand, within RALBFv3_RS10215at 2270.770 kb on + strand, within RALBFv3_RS10215at 2270.827 kb on + strand, within RALBFv3_RS10215at 2270.827 kb on + strand, within RALBFv3_RS10215at 2270.828 kb on - strand, within RALBFv3_RS10215at 2270.828 kb on - strand, within RALBFv3_RS10215at 2270.828 kb on - strand, within RALBFv3_RS10215at 2270.828 kb on - strand, within RALBFv3_RS10215at 2270.828 kb on - strand, within RALBFv3_RS10215at 2270.834 kb on - strand, within RALBFv3_RS10215at 2271.062 kb on - strand, within RALBFv3_RS10215at 2271.067 kb on + strand, within RALBFv3_RS10215at 2271.163 kb on + strand, within RALBFv3_RS10215at 2271.164 kb on - strand, within RALBFv3_RS10215at 2271.167 kb on - strand, within RALBFv3_RS10215at 2271.272 kb on - strandat 2271.368 kb on + strandat 2271.369 kb on - strandat 2271.524 kb on + strand, within RALBFv3_RS10220at 2271.524 kb on + strand, within RALBFv3_RS10220at 2271.525 kb on - strand, within RALBFv3_RS10220at 2271.542 kb on + strand, within RALBFv3_RS10220at 2271.542 kb on + strand, within RALBFv3_RS10220at 2271.542 kb on + strand, within RALBFv3_RS10220at 2271.543 kb on - strand, within RALBFv3_RS10220at 2271.543 kb on - strand, within RALBFv3_RS10220at 2271.543 kb on - strand, within RALBFv3_RS10220at 2271.620 kb on + strand, within RALBFv3_RS10220at 2271.621 kb on - strand, within RALBFv3_RS10220at 2271.621 kb on - strand, within RALBFv3_RS10220at 2271.713 kb on + strand, within RALBFv3_RS10220at 2271.732 kb on - strand, within RALBFv3_RS10220at 2271.899 kb on + strand, within RALBFv3_RS10220at 2271.939 kb on + strand, within RALBFv3_RS10220at 2271.939 kb on + strand, within RALBFv3_RS10220at 2271.950 kb on + strand, within RALBFv3_RS10220at 2271.962 kb on + strand, within RALBFv3_RS10220at 2272.197 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,269,220 + RALBFv3_RS10205 0.79 -0.6
2,269,221 - RALBFv3_RS10205 0.79 +0.1
2,269,221 - RALBFv3_RS10205 0.79 -0.1
2,269,583 + -0.3
2,269,583 + +0.8
2,269,583 + -0.3
2,269,584 - -1.0
2,269,584 - +1.0
2,269,584 - +1.0
2,269,895 + RALBFv3_RS10210 0.64 +1.0
2,269,938 - RALBFv3_RS10210 0.74 -1.1
2,269,938 - RALBFv3_RS10210 0.74 -1.0
2,270,114 - -0.6
2,270,116 + +0.2
2,270,116 + -0.7
2,270,117 - -1.4
2,270,179 + RALBFv3_RS10215 0.10 -0.0
2,270,275 + RALBFv3_RS10215 0.18 -1.2
2,270,275 + RALBFv3_RS10215 0.18 +2.1
2,270,318 - RALBFv3_RS10215 0.21 -1.4
2,270,318 - RALBFv3_RS10215 0.21 +0.1
2,270,341 + RALBFv3_RS10215 0.23 +1.1
2,270,390 - RALBFv3_RS10215 0.27 -1.2
2,270,770 + RALBFv3_RS10215 0.56 -0.5
2,270,827 + RALBFv3_RS10215 0.61 -0.5
2,270,827 + RALBFv3_RS10215 0.61 -1.2
2,270,828 - RALBFv3_RS10215 0.61 -1.9
2,270,828 - RALBFv3_RS10215 0.61 -1.1
2,270,828 - RALBFv3_RS10215 0.61 +1.1
2,270,828 - RALBFv3_RS10215 0.61 -3.0
2,270,828 - RALBFv3_RS10215 0.61 -0.3
2,270,834 - RALBFv3_RS10215 0.61 +1.1
2,271,062 - RALBFv3_RS10215 0.79 +1.1
2,271,067 + RALBFv3_RS10215 0.80 +0.1
2,271,163 + RALBFv3_RS10215 0.87 -2.6
2,271,164 - RALBFv3_RS10215 0.87 -1.4
2,271,167 - RALBFv3_RS10215 0.87 -1.1
2,271,272 - -1.2
2,271,368 + -0.7
2,271,369 - -0.8
2,271,524 + RALBFv3_RS10220 0.19 +0.5
2,271,524 + RALBFv3_RS10220 0.19 -0.8
2,271,525 - RALBFv3_RS10220 0.19 -1.9
2,271,542 + RALBFv3_RS10220 0.21 +1.1
2,271,542 + RALBFv3_RS10220 0.21 -1.3
2,271,542 + RALBFv3_RS10220 0.21 -2.3
2,271,543 - RALBFv3_RS10220 0.21 -1.9
2,271,543 - RALBFv3_RS10220 0.21 -0.3
2,271,543 - RALBFv3_RS10220 0.21 +1.1
2,271,620 + RALBFv3_RS10220 0.29 -1.7
2,271,621 - RALBFv3_RS10220 0.30 +0.1
2,271,621 - RALBFv3_RS10220 0.30 -2.4
2,271,713 + RALBFv3_RS10220 0.39 -1.8
2,271,732 - RALBFv3_RS10220 0.41 -0.9
2,271,899 + RALBFv3_RS10220 0.59 -2.4
2,271,939 + RALBFv3_RS10220 0.63 -0.9
2,271,939 + RALBFv3_RS10220 0.63 -1.8
2,271,950 + RALBFv3_RS10220 0.65 -2.2
2,271,962 + RALBFv3_RS10220 0.66 -3.3
2,272,197 + -0.5

Or see this region's nucleotide sequence