Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10055

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10050 and RALBFv3_RS10055 are separated by 239 nucleotidesRALBFv3_RS10055 and RALBFv3_RS10060 are separated by 22 nucleotidesRALBFv3_RS10060 and RALBFv3_RS10065 are separated by 78 nucleotides RALBFv3_RS10050: RALBFv3_RS10050 - methionine adenosyltransferase, at 2,238,694 to 2,239,884 _RS10050 RALBFv3_RS10055: RALBFv3_RS10055 - lauroyl acyltransferase, at 2,240,124 to 2,240,981 _RS10055 RALBFv3_RS10060: RALBFv3_RS10060 - lauroyl acyltransferase, at 2,241,004 to 2,241,897 _RS10060 RALBFv3_RS10065: RALBFv3_RS10065 - diaminopimelate epimerase, at 2,241,976 to 2,242,854 _RS10065 Position (kb) 2240 2241Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2240.032 kb on - strandat 2240.036 kb on + strandat 2240.036 kb on + strandat 2240.331 kb on + strand, within RALBFv3_RS10055at 2240.683 kb on + strand, within RALBFv3_RS10055at 2240.712 kb on + strand, within RALBFv3_RS10055at 2240.712 kb on + strand, within RALBFv3_RS10055at 2240.910 kb on + strandat 2240.910 kb on + strandat 2240.922 kb on + strandat 2240.979 kb on + strandat 2241.003 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,240,032 - -1.8
2,240,036 + -0.7
2,240,036 + -0.8
2,240,331 + RALBFv3_RS10055 0.24 -2.8
2,240,683 + RALBFv3_RS10055 0.65 -2.4
2,240,712 + RALBFv3_RS10055 0.69 -2.0
2,240,712 + RALBFv3_RS10055 0.69 -2.5
2,240,910 + -2.8
2,240,910 + -4.0
2,240,922 + +1.0
2,240,979 + -1.0
2,241,003 + +0.0

Or see this region's nucleotide sequence