Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS09770

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS09760 and RALBFv3_RS09765 are separated by 253 nucleotidesRALBFv3_RS09765 and RALBFv3_RS09770 are separated by 47 nucleotidesRALBFv3_RS09770 and RALBFv3_RS09775 are separated by 45 nucleotides RALBFv3_RS09760: RALBFv3_RS09760 - LysM peptidoglycan-binding domain-containing protein, at 2,174,374 to 2,175,546 _RS09760 RALBFv3_RS09765: RALBFv3_RS09765 - peptide deformylase, at 2,175,800 to 2,176,309 _RS09765 RALBFv3_RS09770: RALBFv3_RS09770 - membrane protein, at 2,176,357 to 2,178,330 _RS09770 RALBFv3_RS09775: RALBFv3_RS09775 - methionyl-tRNA formyltransferase, at 2,178,376 to 2,179,359 _RS09775 Position (kb) 2176 2177 2178 2179Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2175.556 kb on - strandat 2175.568 kb on + strandat 2175.599 kb on + strandat 2175.599 kb on + strandat 2175.603 kb on + strandat 2175.603 kb on + strandat 2176.349 kb on + strandat 2176.349 kb on + strandat 2176.350 kb on - strandat 2176.415 kb on + strandat 2176.456 kb on + strandat 2176.456 kb on + strandat 2176.456 kb on + strandat 2176.603 kb on + strand, within RALBFv3_RS09770at 2176.603 kb on + strand, within RALBFv3_RS09770at 2176.603 kb on + strand, within RALBFv3_RS09770at 2176.972 kb on + strand, within RALBFv3_RS09770at 2176.972 kb on + strand, within RALBFv3_RS09770at 2177.241 kb on + strand, within RALBFv3_RS09770at 2177.530 kb on + strand, within RALBFv3_RS09770at 2177.530 kb on + strand, within RALBFv3_RS09770at 2177.749 kb on + strand, within RALBFv3_RS09770at 2177.929 kb on + strand, within RALBFv3_RS09770at 2178.028 kb on + strand, within RALBFv3_RS09770

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,175,556 - +1.0
2,175,568 + +0.0
2,175,599 + -0.8
2,175,599 + -0.2
2,175,603 + +0.3
2,175,603 + -0.2
2,176,349 + -0.1
2,176,349 + +0.4
2,176,350 - +1.0
2,176,415 + +0.0
2,176,456 + -1.6
2,176,456 + -2.2
2,176,456 + +1.0
2,176,603 + RALBFv3_RS09770 0.12 -3.0
2,176,603 + RALBFv3_RS09770 0.12 -3.0
2,176,603 + RALBFv3_RS09770 0.12 -2.0
2,176,972 + RALBFv3_RS09770 0.31 -3.0
2,176,972 + RALBFv3_RS09770 0.31 -3.5
2,177,241 + RALBFv3_RS09770 0.45 -1.6
2,177,530 + RALBFv3_RS09770 0.59 -4.7
2,177,530 + RALBFv3_RS09770 0.59 +0.0
2,177,749 + RALBFv3_RS09770 0.71 +1.0
2,177,929 + RALBFv3_RS09770 0.80 -1.3
2,178,028 + RALBFv3_RS09770 0.85 +1.0

Or see this region's nucleotide sequence