Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS09640 and RALBFv3_RS09645 are separated by 88 nucleotides RALBFv3_RS09645 and RALBFv3_RS09650 are separated by 410 nucleotides
RALBFv3_RS09640: RALBFv3_RS09640 - GTP-binding protein, at 2,144,979 to 2,146,277
_RS09640
RALBFv3_RS09645: RALBFv3_RS09645 - RNA polymerase-binding protein DksA, at 2,146,366 to 2,146,785
_RS09645
RALBFv3_RS09650: RALBFv3_RS09650 - GTP-binding protein, at 2,147,196 to 2,148,284
_RS09650
Position (kb)
2146
2147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2145.389 kb on - strand, within RALBFv3_RS09640 at 2145.389 kb on - strand, within RALBFv3_RS09640 at 2145.389 kb on - strand, within RALBFv3_RS09640 at 2145.389 kb on - strand, within RALBFv3_RS09640 at 2145.832 kb on + strand, within RALBFv3_RS09640 at 2145.832 kb on + strand at 2145.832 kb on + strand at 2145.833 kb on - strand, within RALBFv3_RS09640 at 2145.983 kb on - strand, within RALBFv3_RS09640 at 2146.484 kb on + strand, within RALBFv3_RS09645 at 2146.485 kb on - strand, within RALBFv3_RS09645 at 2146.490 kb on + strand, within RALBFv3_RS09645 at 2146.490 kb on + strand, within RALBFv3_RS09645 at 2146.491 kb on - strand, within RALBFv3_RS09645 at 2146.491 kb on - strand, within RALBFv3_RS09645 at 2146.491 kb on - strand, within RALBFv3_RS09645 at 2146.727 kb on + strand, within RALBFv3_RS09645 at 2146.769 kb on - strand at 2146.769 kb on - strand at 2146.769 kb on - strand at 2146.807 kb on + strand at 2146.808 kb on - strand at 2147.078 kb on - strand at 2147.092 kb on + strand at 2147.093 kb on - strand at 2147.093 kb on - strand at 2147.097 kb on + strand at 2147.097 kb on + strand at 2147.097 kb on + strand at 2147.097 kb on + strand at 2147.097 kb on + strand at 2147.098 kb on - strand at 2147.098 kb on - strand at 2147.098 kb on - strand at 2147.112 kb on - strand at 2147.365 kb on + strand, within RALBFv3_RS09650 at 2147.365 kb on + strand, within RALBFv3_RS09650 at 2147.365 kb on + strand, within RALBFv3_RS09650 at 2147.366 kb on - strand, within RALBFv3_RS09650 at 2147.366 kb on - strand, within RALBFv3_RS09650 at 2147.366 kb on - strand, within RALBFv3_RS09650 at 2147.366 kb on - strand, within RALBFv3_RS09650 at 2147.366 kb on - strand, within RALBFv3_RS09650 at 2147.366 kb on - strand, within RALBFv3_RS09650 at 2147.380 kb on + strand, within RALBFv3_RS09650 at 2147.381 kb on - strand, within RALBFv3_RS09650 at 2147.381 kb on - strand, within RALBFv3_RS09650 at 2147.402 kb on - strand, within RALBFv3_RS09650 at 2147.443 kb on + strand, within RALBFv3_RS09650 at 2147.443 kb on + strand, within RALBFv3_RS09650 at 2147.492 kb on + strand, within RALBFv3_RS09650 at 2147.492 kb on + strand, within RALBFv3_RS09650
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,145,389 - RALBFv3_RS09640 0.32 +0.3 2,145,389 - RALBFv3_RS09640 0.32 +0.5 2,145,389 - RALBFv3_RS09640 0.32 -0.4 2,145,389 - RALBFv3_RS09640 0.32 +0.2 2,145,832 + RALBFv3_RS09640 0.66 -0.4 2,145,832 + +0.3 2,145,832 + +1.4 2,145,833 - RALBFv3_RS09640 0.66 -0.2 2,145,983 - RALBFv3_RS09640 0.77 +0.0 2,146,484 + RALBFv3_RS09645 0.28 -1.4 2,146,485 - RALBFv3_RS09645 0.28 -1.3 2,146,490 + RALBFv3_RS09645 0.30 -3.6 2,146,490 + RALBFv3_RS09645 0.30 +1.0 2,146,491 - RALBFv3_RS09645 0.30 -3.4 2,146,491 - RALBFv3_RS09645 0.30 +1.0 2,146,491 - RALBFv3_RS09645 0.30 -2.5 2,146,727 + RALBFv3_RS09645 0.86 -3.0 2,146,769 - -2.5 2,146,769 - -1.8 2,146,769 - -2.7 2,146,807 + -0.4 2,146,808 - -0.5 2,147,078 - -0.1 2,147,092 + -2.8 2,147,093 - -0.6 2,147,093 - -0.2 2,147,097 + -1.0 2,147,097 + -0.9 2,147,097 + -1.9 2,147,097 + -0.5 2,147,097 + +0.4 2,147,098 - +0.3 2,147,098 - +0.2 2,147,098 - +0.0 2,147,112 - -0.4 2,147,365 + RALBFv3_RS09650 0.16 -0.4 2,147,365 + RALBFv3_RS09650 0.16 +0.1 2,147,365 + RALBFv3_RS09650 0.16 +0.3 2,147,366 - RALBFv3_RS09650 0.16 +1.0 2,147,366 - RALBFv3_RS09650 0.16 -0.2 2,147,366 - RALBFv3_RS09650 0.16 +0.6 2,147,366 - RALBFv3_RS09650 0.16 -0.3 2,147,366 - RALBFv3_RS09650 0.16 +0.5 2,147,366 - RALBFv3_RS09650 0.16 +0.2 2,147,380 + RALBFv3_RS09650 0.17 -0.3 2,147,381 - RALBFv3_RS09650 0.17 -0.3 2,147,381 - RALBFv3_RS09650 0.17 -0.1 2,147,402 - RALBFv3_RS09650 0.19 +0.3 2,147,443 + RALBFv3_RS09650 0.23 +0.9 2,147,443 + RALBFv3_RS09650 0.23 -0.8 2,147,492 + RALBFv3_RS09650 0.27 -0.2 2,147,492 + RALBFv3_RS09650 0.27 +1.8
Or see this region's nucleotide sequence