Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS09590

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS09580 and RALBFv3_RS09585 are separated by 175 nucleotidesRALBFv3_RS09585 and RALBFv3_RS09590 are separated by 15 nucleotidesRALBFv3_RS09590 and RALBFv3_RS09595 overlap by 1 nucleotidesRALBFv3_RS09595 and RALBFv3_RS09600 are separated by 32 nucleotidesRALBFv3_RS09600 and RALBFv3_RS09605 are separated by 51 nucleotides RALBFv3_RS09580: RALBFv3_RS09580 - acetylglutamate kinase, at 2,134,485 to 2,135,417 _RS09580 RALBFv3_RS09585: RALBFv3_RS09585 - RNA polymerase sigma factor, at 2,135,593 to 2,136,186 _RS09585 RALBFv3_RS09590: RALBFv3_RS09590 - hypothetical protein, at 2,136,202 to 2,136,417 _RS09590 RALBFv3_RS09595: RALBFv3_RS09595 - hypothetical protein, at 2,136,417 to 2,136,659 _RS09595 RALBFv3_RS09600: RALBFv3_RS09600 - hypothetical protein, at 2,136,692 to 2,136,907 _RS09600 RALBFv3_RS09605: RALBFv3_RS09605 - copper chaperone PCu(A)C, at 2,136,959 to 2,137,459 _RS09605 Position (kb) 2136 2137Strain fitness (log2 ratio) -1 0 1at 2135.445 kb on - strandat 2135.511 kb on + strandat 2135.758 kb on + strand, within RALBFv3_RS09585at 2135.758 kb on + strand, within RALBFv3_RS09585at 2135.758 kb on + strand, within RALBFv3_RS09585at 2135.758 kb on + strand, within RALBFv3_RS09585at 2135.759 kb on - strand, within RALBFv3_RS09585at 2135.759 kb on - strand, within RALBFv3_RS09585at 2135.915 kb on - strand, within RALBFv3_RS09585at 2135.915 kb on - strand, within RALBFv3_RS09585at 2136.115 kb on + strand, within RALBFv3_RS09585at 2136.116 kb on - strand, within RALBFv3_RS09585at 2136.116 kb on - strand, within RALBFv3_RS09585at 2136.331 kb on - strand, within RALBFv3_RS09590at 2136.693 kb on + strandat 2136.901 kb on + strandat 2137.401 kb on - strand, within RALBFv3_RS09605at 2137.401 kb on - strand, within RALBFv3_RS09605

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,135,445 - -1.2
2,135,511 + -0.3
2,135,758 + RALBFv3_RS09585 0.28 +1.0
2,135,758 + RALBFv3_RS09585 0.28 +0.4
2,135,758 + RALBFv3_RS09585 0.28 -0.7
2,135,758 + RALBFv3_RS09585 0.28 +0.1
2,135,759 - RALBFv3_RS09585 0.28 -0.4
2,135,759 - RALBFv3_RS09585 0.28 -1.3
2,135,915 - RALBFv3_RS09585 0.54 +0.5
2,135,915 - RALBFv3_RS09585 0.54 -0.5
2,136,115 + RALBFv3_RS09585 0.88 -0.0
2,136,116 - RALBFv3_RS09585 0.88 -0.0
2,136,116 - RALBFv3_RS09585 0.88 -0.7
2,136,331 - RALBFv3_RS09590 0.60 -0.7
2,136,693 + -0.3
2,136,901 + -0.5
2,137,401 - RALBFv3_RS09605 0.88 -0.9
2,137,401 - RALBFv3_RS09605 0.88 -0.0

Or see this region's nucleotide sequence