Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS09580 and RALBFv3_RS09585 are separated by 175 nucleotides RALBFv3_RS09585 and RALBFv3_RS09590 are separated by 15 nucleotides RALBFv3_RS09590 and RALBFv3_RS09595 overlap by 1 nucleotides RALBFv3_RS09595 and RALBFv3_RS09600 are separated by 32 nucleotides RALBFv3_RS09600 and RALBFv3_RS09605 are separated by 51 nucleotides
RALBFv3_RS09580: RALBFv3_RS09580 - acetylglutamate kinase, at 2,134,485 to 2,135,417
_RS09580
RALBFv3_RS09585: RALBFv3_RS09585 - RNA polymerase sigma factor, at 2,135,593 to 2,136,186
_RS09585
RALBFv3_RS09590: RALBFv3_RS09590 - hypothetical protein, at 2,136,202 to 2,136,417
_RS09590
RALBFv3_RS09595: RALBFv3_RS09595 - hypothetical protein, at 2,136,417 to 2,136,659
_RS09595
RALBFv3_RS09600: RALBFv3_RS09600 - hypothetical protein, at 2,136,692 to 2,136,907
_RS09600
RALBFv3_RS09605: RALBFv3_RS09605 - copper chaperone PCu(A)C, at 2,136,959 to 2,137,459
_RS09605
Position (kb)
2136
2137 Strain fitness (log2 ratio)
-1
0
1 at 2135.445 kb on - strand at 2135.511 kb on + strand at 2135.758 kb on + strand, within RALBFv3_RS09585 at 2135.758 kb on + strand, within RALBFv3_RS09585 at 2135.758 kb on + strand, within RALBFv3_RS09585 at 2135.758 kb on + strand, within RALBFv3_RS09585 at 2135.759 kb on - strand, within RALBFv3_RS09585 at 2135.759 kb on - strand, within RALBFv3_RS09585 at 2135.915 kb on - strand, within RALBFv3_RS09585 at 2135.915 kb on - strand, within RALBFv3_RS09585 at 2136.115 kb on + strand, within RALBFv3_RS09585 at 2136.116 kb on - strand, within RALBFv3_RS09585 at 2136.116 kb on - strand, within RALBFv3_RS09585 at 2136.331 kb on - strand, within RALBFv3_RS09590 at 2136.693 kb on + strand at 2136.901 kb on + strand at 2137.401 kb on - strand, within RALBFv3_RS09605 at 2137.401 kb on - strand, within RALBFv3_RS09605
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,135,445 - -1.2 2,135,511 + -0.3 2,135,758 + RALBFv3_RS09585 0.28 +1.0 2,135,758 + RALBFv3_RS09585 0.28 +0.4 2,135,758 + RALBFv3_RS09585 0.28 -0.7 2,135,758 + RALBFv3_RS09585 0.28 +0.1 2,135,759 - RALBFv3_RS09585 0.28 -0.4 2,135,759 - RALBFv3_RS09585 0.28 -1.3 2,135,915 - RALBFv3_RS09585 0.54 +0.5 2,135,915 - RALBFv3_RS09585 0.54 -0.5 2,136,115 + RALBFv3_RS09585 0.88 -0.0 2,136,116 - RALBFv3_RS09585 0.88 -0.0 2,136,116 - RALBFv3_RS09585 0.88 -0.7 2,136,331 - RALBFv3_RS09590 0.60 -0.7 2,136,693 + -0.3 2,136,901 + -0.5 2,137,401 - RALBFv3_RS09605 0.88 -0.9 2,137,401 - RALBFv3_RS09605 0.88 -0.0
Or see this region's nucleotide sequence