Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS09450

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS09445 and RALBFv3_RS09450 are separated by 15 nucleotidesRALBFv3_RS09450 and RALBFv3_RS09455 are separated by 533 nucleotides RALBFv3_RS09445: RALBFv3_RS09445 - multidrug efflux RND transporter permease subunit, at 2,100,849 to 2,103,998 _RS09445 RALBFv3_RS09450: RALBFv3_RS09450 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 2,104,014 to 2,105,213 _RS09450 RALBFv3_RS09455: RALBFv3_RS09455 - TetR family transcriptional regulator, at 2,105,747 to 2,106,406 _RS09455 Position (kb) 2104 2105 2106Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 2103.016 kb on + strand, within RALBFv3_RS09445at 2103.017 kb on - strand, within RALBFv3_RS09445at 2103.017 kb on - strand, within RALBFv3_RS09445at 2103.029 kb on - strand, within RALBFv3_RS09445at 2103.029 kb on - strand, within RALBFv3_RS09445at 2103.029 kb on - strand, within RALBFv3_RS09445at 2103.049 kb on + strand, within RALBFv3_RS09445at 2103.049 kb on + strand, within RALBFv3_RS09445at 2103.049 kb on + strand, within RALBFv3_RS09445at 2103.050 kb on - strand, within RALBFv3_RS09445at 2103.050 kb on - strand, within RALBFv3_RS09445at 2103.104 kb on + strand, within RALBFv3_RS09445at 2103.172 kb on + strand, within RALBFv3_RS09445at 2103.172 kb on + strand, within RALBFv3_RS09445at 2103.266 kb on - strand, within RALBFv3_RS09445at 2103.385 kb on + strand, within RALBFv3_RS09445at 2103.385 kb on + strand, within RALBFv3_RS09445at 2103.386 kb on - strand, within RALBFv3_RS09445at 2103.386 kb on - strand, within RALBFv3_RS09445at 2103.386 kb on - strand, within RALBFv3_RS09445at 2103.409 kb on + strand, within RALBFv3_RS09445at 2103.409 kb on + strand, within RALBFv3_RS09445at 2103.410 kb on - strand, within RALBFv3_RS09445at 2103.526 kb on + strand, within RALBFv3_RS09445at 2103.526 kb on + strand, within RALBFv3_RS09445at 2103.526 kb on + strand, within RALBFv3_RS09445at 2103.527 kb on - strand, within RALBFv3_RS09445at 2103.527 kb on - strand, within RALBFv3_RS09445at 2103.527 kb on - strand, within RALBFv3_RS09445at 2103.527 kb on - strand, within RALBFv3_RS09445at 2103.527 kb on - strand, within RALBFv3_RS09445at 2103.769 kb on + strandat 2103.769 kb on + strandat 2103.769 kb on + strandat 2103.770 kb on - strandat 2103.771 kb on - strandat 2103.854 kb on - strandat 2103.854 kb on - strandat 2103.854 kb on - strandat 2103.863 kb on + strandat 2103.864 kb on - strandat 2103.864 kb on - strandat 2103.864 kb on - strandat 2103.864 kb on - strandat 2104.015 kb on + strandat 2104.330 kb on + strand, within RALBFv3_RS09450at 2104.330 kb on + strand, within RALBFv3_RS09450at 2104.330 kb on + strand, within RALBFv3_RS09450at 2104.330 kb on + strand, within RALBFv3_RS09450at 2104.330 kb on + strand, within RALBFv3_RS09450at 2104.331 kb on - strand, within RALBFv3_RS09450at 2104.331 kb on - strand, within RALBFv3_RS09450at 2104.331 kb on - strand, within RALBFv3_RS09450at 2104.331 kb on - strand, within RALBFv3_RS09450at 2104.331 kb on - strand, within RALBFv3_RS09450at 2104.423 kb on + strand, within RALBFv3_RS09450at 2104.423 kb on + strand, within RALBFv3_RS09450at 2104.423 kb on + strand, within RALBFv3_RS09450at 2104.424 kb on - strand, within RALBFv3_RS09450at 2104.424 kb on - strand, within RALBFv3_RS09450at 2104.544 kb on - strand, within RALBFv3_RS09450at 2104.570 kb on + strand, within RALBFv3_RS09450at 2104.570 kb on + strand, within RALBFv3_RS09450at 2104.783 kb on + strand, within RALBFv3_RS09450at 2104.783 kb on + strand, within RALBFv3_RS09450at 2104.783 kb on + strand, within RALBFv3_RS09450at 2104.784 kb on - strand, within RALBFv3_RS09450at 2104.825 kb on + strand, within RALBFv3_RS09450at 2104.826 kb on - strand, within RALBFv3_RS09450at 2104.909 kb on + strand, within RALBFv3_RS09450at 2104.909 kb on + strand, within RALBFv3_RS09450at 2104.910 kb on - strand, within RALBFv3_RS09450at 2104.910 kb on - strand, within RALBFv3_RS09450at 2104.969 kb on + strand, within RALBFv3_RS09450at 2104.969 kb on + strand, within RALBFv3_RS09450at 2104.970 kb on - strand, within RALBFv3_RS09450at 2104.970 kb on - strand, within RALBFv3_RS09450at 2104.970 kb on - strand, within RALBFv3_RS09450at 2105.213 kb on + strandat 2105.213 kb on + strandat 2105.213 kb on + strandat 2105.213 kb on + strandat 2105.214 kb on - strandat 2105.214 kb on - strandat 2105.234 kb on - strandat 2105.355 kb on + strandat 2105.385 kb on + strandat 2105.386 kb on - strandat 2105.386 kb on - strandat 2105.386 kb on - strandat 2105.489 kb on + strandat 2105.493 kb on - strandat 2105.512 kb on + strandat 2105.819 kb on + strand, within RALBFv3_RS09455at 2105.891 kb on + strand, within RALBFv3_RS09455at 2105.892 kb on - strand, within RALBFv3_RS09455

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,103,016 + RALBFv3_RS09445 0.69 -3.0
2,103,017 - RALBFv3_RS09445 0.69 -3.8
2,103,017 - RALBFv3_RS09445 0.69 -4.1
2,103,029 - RALBFv3_RS09445 0.69 -4.3
2,103,029 - RALBFv3_RS09445 0.69 +1.0
2,103,029 - RALBFv3_RS09445 0.69 -4.1
2,103,049 + RALBFv3_RS09445 0.70 +1.0
2,103,049 + RALBFv3_RS09445 0.70 -2.3
2,103,049 + RALBFv3_RS09445 0.70 -3.2
2,103,050 - RALBFv3_RS09445 0.70 -2.9
2,103,050 - RALBFv3_RS09445 0.70 -2.5
2,103,104 + RALBFv3_RS09445 0.72 -3.5
2,103,172 + RALBFv3_RS09445 0.74 -4.1
2,103,172 + RALBFv3_RS09445 0.74 -3.7
2,103,266 - RALBFv3_RS09445 0.77 -2.4
2,103,385 + RALBFv3_RS09445 0.81 -3.7
2,103,385 + RALBFv3_RS09445 0.81 -2.9
2,103,386 - RALBFv3_RS09445 0.81 -4.6
2,103,386 - RALBFv3_RS09445 0.81 +1.0
2,103,386 - RALBFv3_RS09445 0.81 -4.4
2,103,409 + RALBFv3_RS09445 0.81 -5.2
2,103,409 + RALBFv3_RS09445 0.81 -4.2
2,103,410 - RALBFv3_RS09445 0.81 -3.2
2,103,526 + RALBFv3_RS09445 0.85 -3.0
2,103,526 + RALBFv3_RS09445 0.85 -4.1
2,103,526 + RALBFv3_RS09445 0.85 -2.8
2,103,527 - RALBFv3_RS09445 0.85 -3.8
2,103,527 - RALBFv3_RS09445 0.85 -3.3
2,103,527 - RALBFv3_RS09445 0.85 -2.0
2,103,527 - RALBFv3_RS09445 0.85 -3.0
2,103,527 - RALBFv3_RS09445 0.85 -1.6
2,103,769 + -4.5
2,103,769 + -3.4
2,103,769 + -0.0
2,103,770 - -4.1
2,103,771 - -3.5
2,103,854 - -3.6
2,103,854 - -4.2
2,103,854 - -4.1
2,103,863 + -5.1
2,103,864 - -2.3
2,103,864 - -3.9
2,103,864 - -4.0
2,103,864 - -5.2
2,104,015 + -2.8
2,104,330 + RALBFv3_RS09450 0.26 -2.0
2,104,330 + RALBFv3_RS09450 0.26 -3.3
2,104,330 + RALBFv3_RS09450 0.26 -4.0
2,104,330 + RALBFv3_RS09450 0.26 -3.2
2,104,330 + RALBFv3_RS09450 0.26 -4.5
2,104,331 - RALBFv3_RS09450 0.26 -4.9
2,104,331 - RALBFv3_RS09450 0.26 -4.6
2,104,331 - RALBFv3_RS09450 0.26 -4.8
2,104,331 - RALBFv3_RS09450 0.26 -4.8
2,104,331 - RALBFv3_RS09450 0.26 -1.8
2,104,423 + RALBFv3_RS09450 0.34 -4.2
2,104,423 + RALBFv3_RS09450 0.34 -4.1
2,104,423 + RALBFv3_RS09450 0.34 -4.3
2,104,424 - RALBFv3_RS09450 0.34 -4.3
2,104,424 - RALBFv3_RS09450 0.34 -4.3
2,104,544 - RALBFv3_RS09450 0.44 -4.8
2,104,570 + RALBFv3_RS09450 0.46 -4.3
2,104,570 + RALBFv3_RS09450 0.46 -5.7
2,104,783 + RALBFv3_RS09450 0.64 -3.3
2,104,783 + RALBFv3_RS09450 0.64 -3.5
2,104,783 + RALBFv3_RS09450 0.64 -4.0
2,104,784 - RALBFv3_RS09450 0.64 -4.9
2,104,825 + RALBFv3_RS09450 0.68 -4.3
2,104,826 - RALBFv3_RS09450 0.68 -2.0
2,104,909 + RALBFv3_RS09450 0.75 -3.9
2,104,909 + RALBFv3_RS09450 0.75 -3.6
2,104,910 - RALBFv3_RS09450 0.75 -4.3
2,104,910 - RALBFv3_RS09450 0.75 -3.9
2,104,969 + RALBFv3_RS09450 0.80 -4.4
2,104,969 + RALBFv3_RS09450 0.80 -4.4
2,104,970 - RALBFv3_RS09450 0.80 -6.1
2,104,970 - RALBFv3_RS09450 0.80 -4.0
2,104,970 - RALBFv3_RS09450 0.80 -5.3
2,105,213 + -2.5
2,105,213 + -4.7
2,105,213 + -3.8
2,105,213 + -3.5
2,105,214 - -4.1
2,105,214 - -3.6
2,105,234 - -0.6
2,105,355 + -4.3
2,105,385 + +1.0
2,105,386 - +1.0
2,105,386 - -0.6
2,105,386 - +0.7
2,105,489 + -3.0
2,105,493 - -0.8
2,105,512 + -4.4
2,105,819 + RALBFv3_RS09455 0.11 +2.4
2,105,891 + RALBFv3_RS09455 0.22 -0.6
2,105,892 - RALBFv3_RS09455 0.22 +2.3

Or see this region's nucleotide sequence