Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS09445 and RALBFv3_RS09450 are separated by 15 nucleotides RALBFv3_RS09450 and RALBFv3_RS09455 are separated by 533 nucleotides
RALBFv3_RS09445: RALBFv3_RS09445 - multidrug efflux RND transporter permease subunit, at 2,100,849 to 2,103,998
_RS09445
RALBFv3_RS09450: RALBFv3_RS09450 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 2,104,014 to 2,105,213
_RS09450
RALBFv3_RS09455: RALBFv3_RS09455 - TetR family transcriptional regulator, at 2,105,747 to 2,106,406
_RS09455
Position (kb)
2104
2105
2106 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2103.016 kb on + strand, within RALBFv3_RS09445 at 2103.017 kb on - strand, within RALBFv3_RS09445 at 2103.017 kb on - strand, within RALBFv3_RS09445 at 2103.029 kb on - strand, within RALBFv3_RS09445 at 2103.029 kb on - strand, within RALBFv3_RS09445 at 2103.029 kb on - strand, within RALBFv3_RS09445 at 2103.049 kb on + strand, within RALBFv3_RS09445 at 2103.049 kb on + strand, within RALBFv3_RS09445 at 2103.049 kb on + strand, within RALBFv3_RS09445 at 2103.050 kb on - strand, within RALBFv3_RS09445 at 2103.050 kb on - strand, within RALBFv3_RS09445 at 2103.104 kb on + strand, within RALBFv3_RS09445 at 2103.172 kb on + strand, within RALBFv3_RS09445 at 2103.172 kb on + strand, within RALBFv3_RS09445 at 2103.266 kb on - strand, within RALBFv3_RS09445 at 2103.385 kb on + strand, within RALBFv3_RS09445 at 2103.385 kb on + strand, within RALBFv3_RS09445 at 2103.386 kb on - strand, within RALBFv3_RS09445 at 2103.386 kb on - strand, within RALBFv3_RS09445 at 2103.386 kb on - strand, within RALBFv3_RS09445 at 2103.409 kb on + strand, within RALBFv3_RS09445 at 2103.409 kb on + strand, within RALBFv3_RS09445 at 2103.410 kb on - strand, within RALBFv3_RS09445 at 2103.526 kb on + strand, within RALBFv3_RS09445 at 2103.526 kb on + strand, within RALBFv3_RS09445 at 2103.526 kb on + strand, within RALBFv3_RS09445 at 2103.527 kb on - strand, within RALBFv3_RS09445 at 2103.527 kb on - strand, within RALBFv3_RS09445 at 2103.527 kb on - strand, within RALBFv3_RS09445 at 2103.527 kb on - strand, within RALBFv3_RS09445 at 2103.527 kb on - strand, within RALBFv3_RS09445 at 2103.769 kb on + strand at 2103.769 kb on + strand at 2103.769 kb on + strand at 2103.770 kb on - strand at 2103.771 kb on - strand at 2103.854 kb on - strand at 2103.854 kb on - strand at 2103.854 kb on - strand at 2103.863 kb on + strand at 2103.864 kb on - strand at 2103.864 kb on - strand at 2103.864 kb on - strand at 2103.864 kb on - strand at 2104.015 kb on + strand at 2104.330 kb on + strand, within RALBFv3_RS09450 at 2104.330 kb on + strand, within RALBFv3_RS09450 at 2104.330 kb on + strand, within RALBFv3_RS09450 at 2104.330 kb on + strand, within RALBFv3_RS09450 at 2104.330 kb on + strand, within RALBFv3_RS09450 at 2104.331 kb on - strand, within RALBFv3_RS09450 at 2104.331 kb on - strand, within RALBFv3_RS09450 at 2104.331 kb on - strand, within RALBFv3_RS09450 at 2104.331 kb on - strand, within RALBFv3_RS09450 at 2104.331 kb on - strand, within RALBFv3_RS09450 at 2104.423 kb on + strand, within RALBFv3_RS09450 at 2104.423 kb on + strand, within RALBFv3_RS09450 at 2104.423 kb on + strand, within RALBFv3_RS09450 at 2104.424 kb on - strand, within RALBFv3_RS09450 at 2104.424 kb on - strand, within RALBFv3_RS09450 at 2104.544 kb on - strand, within RALBFv3_RS09450 at 2104.570 kb on + strand, within RALBFv3_RS09450 at 2104.570 kb on + strand, within RALBFv3_RS09450 at 2104.783 kb on + strand, within RALBFv3_RS09450 at 2104.783 kb on + strand, within RALBFv3_RS09450 at 2104.783 kb on + strand, within RALBFv3_RS09450 at 2104.784 kb on - strand, within RALBFv3_RS09450 at 2104.825 kb on + strand, within RALBFv3_RS09450 at 2104.826 kb on - strand, within RALBFv3_RS09450 at 2104.909 kb on + strand, within RALBFv3_RS09450 at 2104.909 kb on + strand, within RALBFv3_RS09450 at 2104.910 kb on - strand, within RALBFv3_RS09450 at 2104.910 kb on - strand, within RALBFv3_RS09450 at 2104.969 kb on + strand, within RALBFv3_RS09450 at 2104.969 kb on + strand, within RALBFv3_RS09450 at 2104.970 kb on - strand, within RALBFv3_RS09450 at 2104.970 kb on - strand, within RALBFv3_RS09450 at 2104.970 kb on - strand, within RALBFv3_RS09450 at 2105.213 kb on + strand at 2105.213 kb on + strand at 2105.213 kb on + strand at 2105.213 kb on + strand at 2105.214 kb on - strand at 2105.214 kb on - strand at 2105.234 kb on - strand at 2105.355 kb on + strand at 2105.385 kb on + strand at 2105.386 kb on - strand at 2105.386 kb on - strand at 2105.386 kb on - strand at 2105.489 kb on + strand at 2105.493 kb on - strand at 2105.512 kb on + strand at 2105.819 kb on + strand, within RALBFv3_RS09455 at 2105.891 kb on + strand, within RALBFv3_RS09455 at 2105.892 kb on - strand, within RALBFv3_RS09455
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,103,016 + RALBFv3_RS09445 0.69 -3.0 2,103,017 - RALBFv3_RS09445 0.69 -3.8 2,103,017 - RALBFv3_RS09445 0.69 -4.1 2,103,029 - RALBFv3_RS09445 0.69 -4.3 2,103,029 - RALBFv3_RS09445 0.69 +1.0 2,103,029 - RALBFv3_RS09445 0.69 -4.1 2,103,049 + RALBFv3_RS09445 0.70 +1.0 2,103,049 + RALBFv3_RS09445 0.70 -2.3 2,103,049 + RALBFv3_RS09445 0.70 -3.2 2,103,050 - RALBFv3_RS09445 0.70 -2.9 2,103,050 - RALBFv3_RS09445 0.70 -2.5 2,103,104 + RALBFv3_RS09445 0.72 -3.5 2,103,172 + RALBFv3_RS09445 0.74 -4.1 2,103,172 + RALBFv3_RS09445 0.74 -3.7 2,103,266 - RALBFv3_RS09445 0.77 -2.4 2,103,385 + RALBFv3_RS09445 0.81 -3.7 2,103,385 + RALBFv3_RS09445 0.81 -2.9 2,103,386 - RALBFv3_RS09445 0.81 -4.6 2,103,386 - RALBFv3_RS09445 0.81 +1.0 2,103,386 - RALBFv3_RS09445 0.81 -4.4 2,103,409 + RALBFv3_RS09445 0.81 -5.2 2,103,409 + RALBFv3_RS09445 0.81 -4.2 2,103,410 - RALBFv3_RS09445 0.81 -3.2 2,103,526 + RALBFv3_RS09445 0.85 -3.0 2,103,526 + RALBFv3_RS09445 0.85 -4.1 2,103,526 + RALBFv3_RS09445 0.85 -2.8 2,103,527 - RALBFv3_RS09445 0.85 -3.8 2,103,527 - RALBFv3_RS09445 0.85 -3.3 2,103,527 - RALBFv3_RS09445 0.85 -2.0 2,103,527 - RALBFv3_RS09445 0.85 -3.0 2,103,527 - RALBFv3_RS09445 0.85 -1.6 2,103,769 + -4.5 2,103,769 + -3.4 2,103,769 + -0.0 2,103,770 - -4.1 2,103,771 - -3.5 2,103,854 - -3.6 2,103,854 - -4.2 2,103,854 - -4.1 2,103,863 + -5.1 2,103,864 - -2.3 2,103,864 - -3.9 2,103,864 - -4.0 2,103,864 - -5.2 2,104,015 + -2.8 2,104,330 + RALBFv3_RS09450 0.26 -2.0 2,104,330 + RALBFv3_RS09450 0.26 -3.3 2,104,330 + RALBFv3_RS09450 0.26 -4.0 2,104,330 + RALBFv3_RS09450 0.26 -3.2 2,104,330 + RALBFv3_RS09450 0.26 -4.5 2,104,331 - RALBFv3_RS09450 0.26 -4.9 2,104,331 - RALBFv3_RS09450 0.26 -4.6 2,104,331 - RALBFv3_RS09450 0.26 -4.8 2,104,331 - RALBFv3_RS09450 0.26 -4.8 2,104,331 - RALBFv3_RS09450 0.26 -1.8 2,104,423 + RALBFv3_RS09450 0.34 -4.2 2,104,423 + RALBFv3_RS09450 0.34 -4.1 2,104,423 + RALBFv3_RS09450 0.34 -4.3 2,104,424 - RALBFv3_RS09450 0.34 -4.3 2,104,424 - RALBFv3_RS09450 0.34 -4.3 2,104,544 - RALBFv3_RS09450 0.44 -4.8 2,104,570 + RALBFv3_RS09450 0.46 -4.3 2,104,570 + RALBFv3_RS09450 0.46 -5.7 2,104,783 + RALBFv3_RS09450 0.64 -3.3 2,104,783 + RALBFv3_RS09450 0.64 -3.5 2,104,783 + RALBFv3_RS09450 0.64 -4.0 2,104,784 - RALBFv3_RS09450 0.64 -4.9 2,104,825 + RALBFv3_RS09450 0.68 -4.3 2,104,826 - RALBFv3_RS09450 0.68 -2.0 2,104,909 + RALBFv3_RS09450 0.75 -3.9 2,104,909 + RALBFv3_RS09450 0.75 -3.6 2,104,910 - RALBFv3_RS09450 0.75 -4.3 2,104,910 - RALBFv3_RS09450 0.75 -3.9 2,104,969 + RALBFv3_RS09450 0.80 -4.4 2,104,969 + RALBFv3_RS09450 0.80 -4.4 2,104,970 - RALBFv3_RS09450 0.80 -6.1 2,104,970 - RALBFv3_RS09450 0.80 -4.0 2,104,970 - RALBFv3_RS09450 0.80 -5.3 2,105,213 + -2.5 2,105,213 + -4.7 2,105,213 + -3.8 2,105,213 + -3.5 2,105,214 - -4.1 2,105,214 - -3.6 2,105,234 - -0.6 2,105,355 + -4.3 2,105,385 + +1.0 2,105,386 - +1.0 2,105,386 - -0.6 2,105,386 - +0.7 2,105,489 + -3.0 2,105,493 - -0.8 2,105,512 + -4.4 2,105,819 + RALBFv3_RS09455 0.11 +2.4 2,105,891 + RALBFv3_RS09455 0.22 -0.6 2,105,892 - RALBFv3_RS09455 0.22 +2.3
Or see this region's nucleotide sequence