Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS09435 and RALBFv3_RS09440 are separated by 529 nucleotides RALBFv3_RS09440 and RALBFv3_RS09445 are separated by 2 nucleotides
RALBFv3_RS09435: RALBFv3_RS09435 - 30S ribosomal protein S21, at 2,098,560 to 2,098,772
_RS09435
RALBFv3_RS09440: RALBFv3_RS09440 - multidrug transporter, at 2,099,302 to 2,100,846
_RS09440
RALBFv3_RS09445: RALBFv3_RS09445 - multidrug efflux RND transporter permease subunit, at 2,100,849 to 2,103,998
_RS09445
Position (kb)
2099
2100
2101 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2098.393 kb on - strand at 2098.561 kb on + strand at 2098.821 kb on - strand at 2098.823 kb on - strand at 2098.935 kb on + strand at 2098.938 kb on + strand at 2098.938 kb on + strand at 2098.939 kb on - strand at 2098.939 kb on - strand at 2099.124 kb on + strand at 2099.166 kb on + strand at 2099.210 kb on + strand at 2099.210 kb on + strand at 2099.303 kb on + strand at 2099.304 kb on - strand at 2099.304 kb on - strand at 2099.426 kb on + strand at 2099.426 kb on + strand at 2099.543 kb on + strand, within RALBFv3_RS09440 at 2099.681 kb on + strand, within RALBFv3_RS09440 at 2099.681 kb on + strand, within RALBFv3_RS09440 at 2099.681 kb on + strand, within RALBFv3_RS09440 at 2099.681 kb on + strand, within RALBFv3_RS09440 at 2099.681 kb on + strand, within RALBFv3_RS09440 at 2099.681 kb on + strand, within RALBFv3_RS09440 at 2099.682 kb on - strand, within RALBFv3_RS09440 at 2099.682 kb on - strand, within RALBFv3_RS09440 at 2099.682 kb on - strand, within RALBFv3_RS09440 at 2100.077 kb on + strand, within RALBFv3_RS09440 at 2100.078 kb on - strand, within RALBFv3_RS09440 at 2100.078 kb on - strand, within RALBFv3_RS09440 at 2100.191 kb on + strand, within RALBFv3_RS09440 at 2100.191 kb on + strand, within RALBFv3_RS09440 at 2100.191 kb on + strand, within RALBFv3_RS09440 at 2100.191 kb on + strand, within RALBFv3_RS09440 at 2100.191 kb on + strand, within RALBFv3_RS09440 at 2100.191 kb on + strand, within RALBFv3_RS09440 at 2100.192 kb on - strand, within RALBFv3_RS09440 at 2100.192 kb on - strand, within RALBFv3_RS09440 at 2100.192 kb on - strand, within RALBFv3_RS09440 at 2100.194 kb on + strand, within RALBFv3_RS09440 at 2100.194 kb on + strand, within RALBFv3_RS09440 at 2100.329 kb on + strand, within RALBFv3_RS09440 at 2100.329 kb on + strand, within RALBFv3_RS09440 at 2100.330 kb on - strand, within RALBFv3_RS09440 at 2100.383 kb on + strand, within RALBFv3_RS09440 at 2100.383 kb on + strand, within RALBFv3_RS09440 at 2100.383 kb on + strand, within RALBFv3_RS09440 at 2100.383 kb on + strand, within RALBFv3_RS09440 at 2100.384 kb on - strand, within RALBFv3_RS09440 at 2100.410 kb on + strand, within RALBFv3_RS09440 at 2100.411 kb on - strand, within RALBFv3_RS09440 at 2100.473 kb on + strand, within RALBFv3_RS09440 at 2100.474 kb on - strand, within RALBFv3_RS09440 at 2100.546 kb on + strand, within RALBFv3_RS09440 at 2100.698 kb on + strand at 2100.753 kb on - strand at 2100.842 kb on + strand at 2100.842 kb on + strand at 2100.842 kb on + strand at 2100.842 kb on + strand at 2100.842 kb on + strand at 2100.843 kb on - strand at 2100.843 kb on - strand at 2100.843 kb on - strand at 2100.843 kb on - strand at 2101.231 kb on + strand, within RALBFv3_RS09445 at 2101.231 kb on + strand, within RALBFv3_RS09445 at 2101.231 kb on + strand, within RALBFv3_RS09445 at 2101.231 kb on + strand, within RALBFv3_RS09445 at 2101.232 kb on + strand, within RALBFv3_RS09445 at 2101.232 kb on - strand, within RALBFv3_RS09445 at 2101.232 kb on - strand, within RALBFv3_RS09445 at 2101.232 kb on - strand, within RALBFv3_RS09445 at 2101.232 kb on - strand, within RALBFv3_RS09445 at 2101.232 kb on - strand, within RALBFv3_RS09445 at 2101.232 kb on - strand, within RALBFv3_RS09445 at 2101.333 kb on + strand, within RALBFv3_RS09445 at 2101.333 kb on + strand, within RALBFv3_RS09445 at 2101.334 kb on - strand, within RALBFv3_RS09445 at 2101.334 kb on - strand, within RALBFv3_RS09445 at 2101.334 kb on - strand, within RALBFv3_RS09445 at 2101.334 kb on - strand, within RALBFv3_RS09445 at 2101.379 kb on - strand, within RALBFv3_RS09445 at 2101.553 kb on - strand, within RALBFv3_RS09445 at 2101.714 kb on + strand, within RALBFv3_RS09445 at 2101.714 kb on + strand, within RALBFv3_RS09445 at 2101.714 kb on + strand, within RALBFv3_RS09445 at 2101.715 kb on - strand, within RALBFv3_RS09445 at 2101.715 kb on - strand, within RALBFv3_RS09445 at 2101.736 kb on - strand, within RALBFv3_RS09445 at 2101.829 kb on - strand, within RALBFv3_RS09445
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,098,393 - +1.0 2,098,561 + -0.6 2,098,821 - +0.3 2,098,823 - +0.2 2,098,935 + +0.2 2,098,938 + -1.6 2,098,938 + +1.0 2,098,939 - +0.6 2,098,939 - +1.4 2,099,124 + -0.7 2,099,166 + -0.0 2,099,210 + +1.3 2,099,210 + -0.4 2,099,303 + -0.9 2,099,304 - -0.4 2,099,304 - -0.6 2,099,426 + -3.0 2,099,426 + -3.3 2,099,543 + RALBFv3_RS09440 0.16 -3.4 2,099,681 + RALBFv3_RS09440 0.25 -4.1 2,099,681 + RALBFv3_RS09440 0.25 -1.0 2,099,681 + RALBFv3_RS09440 0.25 -4.6 2,099,681 + RALBFv3_RS09440 0.25 -4.1 2,099,681 + RALBFv3_RS09440 0.25 -2.0 2,099,681 + RALBFv3_RS09440 0.25 -2.0 2,099,682 - RALBFv3_RS09440 0.25 -3.4 2,099,682 - RALBFv3_RS09440 0.25 -3.0 2,099,682 - RALBFv3_RS09440 0.25 -1.2 2,100,077 + RALBFv3_RS09440 0.50 -3.8 2,100,078 - RALBFv3_RS09440 0.50 -5.7 2,100,078 - RALBFv3_RS09440 0.50 -4.3 2,100,191 + RALBFv3_RS09440 0.58 -1.0 2,100,191 + RALBFv3_RS09440 0.58 -3.3 2,100,191 + RALBFv3_RS09440 0.58 -4.3 2,100,191 + RALBFv3_RS09440 0.58 -1.6 2,100,191 + RALBFv3_RS09440 0.58 +1.0 2,100,191 + RALBFv3_RS09440 0.58 -4.1 2,100,192 - RALBFv3_RS09440 0.58 -4.2 2,100,192 - RALBFv3_RS09440 0.58 -4.7 2,100,192 - RALBFv3_RS09440 0.58 -4.8 2,100,194 + RALBFv3_RS09440 0.58 -3.5 2,100,194 + RALBFv3_RS09440 0.58 -5.0 2,100,329 + RALBFv3_RS09440 0.66 -3.4 2,100,329 + RALBFv3_RS09440 0.66 -2.0 2,100,330 - RALBFv3_RS09440 0.67 -4.1 2,100,383 + RALBFv3_RS09440 0.70 -2.0 2,100,383 + RALBFv3_RS09440 0.70 +1.0 2,100,383 + RALBFv3_RS09440 0.70 -2.5 2,100,383 + RALBFv3_RS09440 0.70 -3.7 2,100,384 - RALBFv3_RS09440 0.70 -3.9 2,100,410 + RALBFv3_RS09440 0.72 -3.8 2,100,411 - RALBFv3_RS09440 0.72 -2.0 2,100,473 + RALBFv3_RS09440 0.76 -4.7 2,100,474 - RALBFv3_RS09440 0.76 -4.7 2,100,546 + RALBFv3_RS09440 0.81 -3.1 2,100,698 + -3.9 2,100,753 - -2.2 2,100,842 + -2.1 2,100,842 + -4.4 2,100,842 + -3.2 2,100,842 + -4.8 2,100,842 + -1.3 2,100,843 - -2.1 2,100,843 - -3.6 2,100,843 - -2.3 2,100,843 - -3.1 2,101,231 + RALBFv3_RS09445 0.12 -4.4 2,101,231 + RALBFv3_RS09445 0.12 -3.6 2,101,231 + RALBFv3_RS09445 0.12 -4.6 2,101,231 + RALBFv3_RS09445 0.12 -3.4 2,101,232 + RALBFv3_RS09445 0.12 -5.1 2,101,232 - RALBFv3_RS09445 0.12 -5.0 2,101,232 - RALBFv3_RS09445 0.12 -4.1 2,101,232 - RALBFv3_RS09445 0.12 -4.1 2,101,232 - RALBFv3_RS09445 0.12 -2.6 2,101,232 - RALBFv3_RS09445 0.12 -5.6 2,101,232 - RALBFv3_RS09445 0.12 -4.6 2,101,333 + RALBFv3_RS09445 0.15 +1.0 2,101,333 + RALBFv3_RS09445 0.15 -5.4 2,101,334 - RALBFv3_RS09445 0.15 -4.5 2,101,334 - RALBFv3_RS09445 0.15 -4.2 2,101,334 - RALBFv3_RS09445 0.15 -2.5 2,101,334 - RALBFv3_RS09445 0.15 -3.3 2,101,379 - RALBFv3_RS09445 0.17 -3.8 2,101,553 - RALBFv3_RS09445 0.22 -4.2 2,101,714 + RALBFv3_RS09445 0.27 -5.5 2,101,714 + RALBFv3_RS09445 0.27 -2.7 2,101,714 + RALBFv3_RS09445 0.27 -2.6 2,101,715 - RALBFv3_RS09445 0.27 -3.0 2,101,715 - RALBFv3_RS09445 0.27 -4.0 2,101,736 - RALBFv3_RS09445 0.28 -3.4 2,101,829 - RALBFv3_RS09445 0.31 -4.6
Or see this region's nucleotide sequence