Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS08975 and RALBFv3_RS08980 are separated by 34 nucleotides RALBFv3_RS08980 and RALBFv3_RS08985 are separated by 74 nucleotides RALBFv3_RS08985 and RALBFv3_RS08990 are separated by 140 nucleotides
RALBFv3_RS08975: RALBFv3_RS08975 - ornithine cyclodeaminase, at 1,980,505 to 1,981,509
_RS08975
RALBFv3_RS08980: RALBFv3_RS08980 - lysoplasmalogenase, at 1,981,544 to 1,982,311
_RS08980
RALBFv3_RS08985: RALBFv3_RS08985 - Lrp/AsnC family transcriptional regulator, at 1,982,386 to 1,982,862
_RS08985
RALBFv3_RS08990: RALBFv3_RS08990 - phenylalanine 4-monooxygenase, at 1,983,003 to 1,983,944
_RS08990
Position (kb) 1981
1982
1983 Strain fitness (log2 ratio) -1
0
1 at 1981.464 kb on - strand at 1981.483 kb on - strand at 1981.850 kb on - strand, within RALBFv3_RS08980 at 1981.935 kb on + strand, within RALBFv3_RS08980 at 1982.199 kb on + strand, within RALBFv3_RS08980 at 1982.199 kb on + strand, within RALBFv3_RS08980 at 1982.200 kb on - strand, within RALBFv3_RS08980 at 1982.331 kb on + strand at 1982.331 kb on + strand at 1982.331 kb on + strand at 1982.525 kb on - strand, within RALBFv3_RS08985 at 1982.543 kb on + strand, within RALBFv3_RS08985 at 1982.543 kb on + strand, within RALBFv3_RS08985 at 1982.544 kb on - strand, within RALBFv3_RS08985 at 1982.567 kb on + strand, within RALBFv3_RS08985 at 1982.568 kb on - strand, within RALBFv3_RS08985 at 1982.960 kb on + strand at 1982.961 kb on - strand at 1982.995 kb on + strand at 1982.998 kb on - strand at 1983.157 kb on - strand, within RALBFv3_RS08990 at 1983.192 kb on + strand, within RALBFv3_RS08990
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,981,464 - -0.6 1,981,483 - -0.2 1,981,850 - RALBFv3_RS08980 0.40 +1.4 1,981,935 + RALBFv3_RS08980 0.51 -1.2 1,982,199 + RALBFv3_RS08980 0.85 -0.4 1,982,199 + RALBFv3_RS08980 0.85 -1.2 1,982,200 - RALBFv3_RS08980 0.85 -0.8 1,982,331 + -0.8 1,982,331 + -1.0 1,982,331 + -1.0 1,982,525 - RALBFv3_RS08985 0.29 +1.0 1,982,543 + RALBFv3_RS08985 0.33 -0.4 1,982,543 + RALBFv3_RS08985 0.33 -0.1 1,982,544 - RALBFv3_RS08985 0.33 +0.8 1,982,567 + RALBFv3_RS08985 0.38 -1.6 1,982,568 - RALBFv3_RS08985 0.38 +0.7 1,982,960 + -0.2 1,982,961 - +1.0 1,982,995 + -0.3 1,982,998 - +0.3 1,983,157 - RALBFv3_RS08990 0.16 +0.6 1,983,192 + RALBFv3_RS08990 0.20 -0.6
Or see this region's nucleotide sequence