Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS08635

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS08625 and RALBFv3_RS08630 are separated by 106 nucleotidesRALBFv3_RS08630 and RALBFv3_RS08635 are separated by 497 nucleotidesRALBFv3_RS08635 and RALBFv3_RS08640 are separated by 244 nucleotidesRALBFv3_RS08640 and RALBFv3_RS08645 are separated by 148 nucleotides RALBFv3_RS08625: RALBFv3_RS08625 - hypothetical protein, at 1,896,925 to 1,897,428 _RS08625 RALBFv3_RS08630: RALBFv3_RS08630 - sugar-binding lectin protein, at 1,897,535 to 1,897,879 _RS08630 RALBFv3_RS08635: RALBFv3_RS08635 - ATP-dependent DNA helicase Rep, at 1,898,377 to 1,900,494 _RS08635 RALBFv3_RS08640: RALBFv3_RS08640 - hypothetical protein, at 1,900,739 to 1,901,065 _RS08640 RALBFv3_RS08645: RALBFv3_RS08645 - membrane protein, at 1,901,214 to 1,902,224 _RS08645 Position (kb) 1898 1899 1900 1901Strain fitness (log2 ratio) -2 -1 0 1at 1897.432 kb on - strandat 1897.501 kb on + strandat 1897.502 kb on - strandat 1897.575 kb on + strand, within RALBFv3_RS08630at 1897.862 kb on + strandat 1897.863 kb on - strandat 1897.863 kb on - strandat 1897.863 kb on - strandat 1897.880 kb on - strandat 1898.011 kb on + strandat 1898.011 kb on + strandat 1898.011 kb on + strandat 1898.016 kb on + strandat 1898.016 kb on + strandat 1898.016 kb on + strandat 1898.039 kb on + strandat 1898.039 kb on + strandat 1898.039 kb on + strandat 1898.039 kb on + strandat 1898.039 kb on + strandat 1898.040 kb on - strandat 1898.040 kb on - strandat 1898.040 kb on - strandat 1898.060 kb on - strandat 1898.253 kb on + strandat 1898.253 kb on + strandat 1898.253 kb on + strandat 1898.254 kb on - strandat 1898.294 kb on - strandat 1898.378 kb on - strandat 1898.562 kb on + strandat 1898.563 kb on - strandat 1898.600 kb on + strand, within RALBFv3_RS08635at 1898.600 kb on + strand, within RALBFv3_RS08635at 1898.601 kb on - strand, within RALBFv3_RS08635at 1898.706 kb on - strand, within RALBFv3_RS08635at 1898.963 kb on + strand, within RALBFv3_RS08635at 1898.972 kb on + strand, within RALBFv3_RS08635at 1898.973 kb on - strand, within RALBFv3_RS08635at 1898.973 kb on - strand, within RALBFv3_RS08635at 1898.978 kb on + strand, within RALBFv3_RS08635at 1899.336 kb on - strand, within RALBFv3_RS08635at 1899.500 kb on - strand, within RALBFv3_RS08635at 1899.611 kb on + strand, within RALBFv3_RS08635at 1899.611 kb on + strand, within RALBFv3_RS08635at 1899.611 kb on + strand, within RALBFv3_RS08635at 1899.612 kb on - strand, within RALBFv3_RS08635at 1899.612 kb on - strand, within RALBFv3_RS08635at 1899.698 kb on + strand, within RALBFv3_RS08635at 1899.699 kb on - strand, within RALBFv3_RS08635at 1899.782 kb on + strand, within RALBFv3_RS08635at 1899.783 kb on - strand, within RALBFv3_RS08635at 1899.825 kb on - strand, within RALBFv3_RS08635at 1899.941 kb on + strand, within RALBFv3_RS08635at 1900.206 kb on + strand, within RALBFv3_RS08635at 1900.207 kb on - strand, within RALBFv3_RS08635at 1900.207 kb on - strand, within RALBFv3_RS08635at 1900.439 kb on + strandat 1900.440 kb on - strandat 1900.538 kb on + strandat 1900.538 kb on + strandat 1900.538 kb on + strandat 1900.539 kb on - strandat 1900.539 kb on - strandat 1900.539 kb on - strandat 1900.539 kb on - strandat 1900.777 kb on + strand, within RALBFv3_RS08640at 1900.778 kb on - strand, within RALBFv3_RS08640at 1901.215 kb on + strandat 1901.215 kb on + strandat 1901.215 kb on + strandat 1901.216 kb on - strandat 1901.216 kb on - strandat 1901.216 kb on - strandat 1901.216 kb on - strandat 1901.233 kb on + strandat 1901.234 kb on - strandat 1901.320 kb on + strand, within RALBFv3_RS08645at 1901.320 kb on + strand, within RALBFv3_RS08645at 1901.321 kb on - strand, within RALBFv3_RS08645at 1901.339 kb on - strand, within RALBFv3_RS08645at 1901.380 kb on + strand, within RALBFv3_RS08645at 1901.481 kb on + strand, within RALBFv3_RS08645

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,897,432 - -0.7
1,897,501 + -0.2
1,897,502 - +0.1
1,897,575 + RALBFv3_RS08630 0.12 -0.0
1,897,862 + +1.0
1,897,863 - -0.6
1,897,863 - +1.0
1,897,863 - +0.6
1,897,880 - -0.4
1,898,011 + +1.0
1,898,011 + -0.2
1,898,011 + -0.3
1,898,016 + +1.0
1,898,016 + +0.1
1,898,016 + +0.6
1,898,039 + +0.3
1,898,039 + -0.2
1,898,039 + -0.0
1,898,039 + -0.5
1,898,039 + -0.3
1,898,040 - -0.4
1,898,040 - -0.3
1,898,040 - -1.0
1,898,060 - +0.4
1,898,253 + -0.2
1,898,253 + -0.8
1,898,253 + +0.6
1,898,254 - +1.0
1,898,294 - +0.1
1,898,378 - -0.6
1,898,562 + -0.6
1,898,563 - -1.8
1,898,600 + RALBFv3_RS08635 0.11 +0.4
1,898,600 + RALBFv3_RS08635 0.11 +1.0
1,898,601 - RALBFv3_RS08635 0.11 +1.6
1,898,706 - RALBFv3_RS08635 0.16 -1.8
1,898,963 + RALBFv3_RS08635 0.28 +1.0
1,898,972 + RALBFv3_RS08635 0.28 -0.6
1,898,973 - RALBFv3_RS08635 0.28 +1.0
1,898,973 - RALBFv3_RS08635 0.28 -1.7
1,898,978 + RALBFv3_RS08635 0.28 +1.0
1,899,336 - RALBFv3_RS08635 0.45 -0.6
1,899,500 - RALBFv3_RS08635 0.53 -0.0
1,899,611 + RALBFv3_RS08635 0.58 -0.3
1,899,611 + RALBFv3_RS08635 0.58 +1.0
1,899,611 + RALBFv3_RS08635 0.58 -1.0
1,899,612 - RALBFv3_RS08635 0.58 +1.0
1,899,612 - RALBFv3_RS08635 0.58 -0.0
1,899,698 + RALBFv3_RS08635 0.62 -1.0
1,899,699 - RALBFv3_RS08635 0.62 -1.1
1,899,782 + RALBFv3_RS08635 0.66 +1.0
1,899,783 - RALBFv3_RS08635 0.66 -0.6
1,899,825 - RALBFv3_RS08635 0.68 -0.5
1,899,941 + RALBFv3_RS08635 0.74 -0.6
1,900,206 + RALBFv3_RS08635 0.86 +0.4
1,900,207 - RALBFv3_RS08635 0.86 -0.6
1,900,207 - RALBFv3_RS08635 0.86 -1.4
1,900,439 + -0.3
1,900,440 - +1.4
1,900,538 + -0.0
1,900,538 + -1.3
1,900,538 + +0.1
1,900,539 - -1.2
1,900,539 - +0.8
1,900,539 - -0.0
1,900,539 - +1.0
1,900,777 + RALBFv3_RS08640 0.12 -0.6
1,900,778 - RALBFv3_RS08640 0.12 -0.4
1,901,215 + +0.0
1,901,215 + -0.2
1,901,215 + +0.3
1,901,216 - -0.4
1,901,216 - +0.4
1,901,216 - -0.2
1,901,216 - -0.1
1,901,233 + +1.0
1,901,234 - +1.6
1,901,320 + RALBFv3_RS08645 0.10 +1.0
1,901,320 + RALBFv3_RS08645 0.10 -0.1
1,901,321 - RALBFv3_RS08645 0.11 -0.4
1,901,339 - RALBFv3_RS08645 0.12 -0.3
1,901,380 + RALBFv3_RS08645 0.16 -0.0
1,901,481 + RALBFv3_RS08645 0.26 -0.5

Or see this region's nucleotide sequence