Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS08625 and RALBFv3_RS08630 are separated by 106 nucleotides RALBFv3_RS08630 and RALBFv3_RS08635 are separated by 497 nucleotides RALBFv3_RS08635 and RALBFv3_RS08640 are separated by 244 nucleotides RALBFv3_RS08640 and RALBFv3_RS08645 are separated by 148 nucleotides
RALBFv3_RS08625: RALBFv3_RS08625 - hypothetical protein, at 1,896,925 to 1,897,428
_RS08625
RALBFv3_RS08630: RALBFv3_RS08630 - sugar-binding lectin protein, at 1,897,535 to 1,897,879
_RS08630
RALBFv3_RS08635: RALBFv3_RS08635 - ATP-dependent DNA helicase Rep, at 1,898,377 to 1,900,494
_RS08635
RALBFv3_RS08640: RALBFv3_RS08640 - hypothetical protein, at 1,900,739 to 1,901,065
_RS08640
RALBFv3_RS08645: RALBFv3_RS08645 - membrane protein, at 1,901,214 to 1,902,224
_RS08645
Position (kb)
1898
1899
1900
1901 Strain fitness (log2 ratio)
-2
-1
0
1 at 1897.432 kb on - strand at 1897.501 kb on + strand at 1897.502 kb on - strand at 1897.575 kb on + strand, within RALBFv3_RS08630 at 1897.862 kb on + strand at 1897.863 kb on - strand at 1897.863 kb on - strand at 1897.863 kb on - strand at 1897.880 kb on - strand at 1898.011 kb on + strand at 1898.011 kb on + strand at 1898.011 kb on + strand at 1898.016 kb on + strand at 1898.016 kb on + strand at 1898.016 kb on + strand at 1898.039 kb on + strand at 1898.039 kb on + strand at 1898.039 kb on + strand at 1898.039 kb on + strand at 1898.039 kb on + strand at 1898.040 kb on - strand at 1898.040 kb on - strand at 1898.040 kb on - strand at 1898.060 kb on - strand at 1898.253 kb on + strand at 1898.253 kb on + strand at 1898.253 kb on + strand at 1898.254 kb on - strand at 1898.294 kb on - strand at 1898.378 kb on - strand at 1898.562 kb on + strand at 1898.563 kb on - strand at 1898.600 kb on + strand, within RALBFv3_RS08635 at 1898.600 kb on + strand, within RALBFv3_RS08635 at 1898.601 kb on - strand, within RALBFv3_RS08635 at 1898.706 kb on - strand, within RALBFv3_RS08635 at 1898.963 kb on + strand, within RALBFv3_RS08635 at 1898.972 kb on + strand, within RALBFv3_RS08635 at 1898.973 kb on - strand, within RALBFv3_RS08635 at 1898.973 kb on - strand, within RALBFv3_RS08635 at 1898.978 kb on + strand, within RALBFv3_RS08635 at 1899.336 kb on - strand, within RALBFv3_RS08635 at 1899.500 kb on - strand, within RALBFv3_RS08635 at 1899.611 kb on + strand, within RALBFv3_RS08635 at 1899.611 kb on + strand, within RALBFv3_RS08635 at 1899.611 kb on + strand, within RALBFv3_RS08635 at 1899.612 kb on - strand, within RALBFv3_RS08635 at 1899.612 kb on - strand, within RALBFv3_RS08635 at 1899.698 kb on + strand, within RALBFv3_RS08635 at 1899.699 kb on - strand, within RALBFv3_RS08635 at 1899.782 kb on + strand, within RALBFv3_RS08635 at 1899.783 kb on - strand, within RALBFv3_RS08635 at 1899.825 kb on - strand, within RALBFv3_RS08635 at 1899.941 kb on + strand, within RALBFv3_RS08635 at 1900.206 kb on + strand, within RALBFv3_RS08635 at 1900.207 kb on - strand, within RALBFv3_RS08635 at 1900.207 kb on - strand, within RALBFv3_RS08635 at 1900.439 kb on + strand at 1900.440 kb on - strand at 1900.538 kb on + strand at 1900.538 kb on + strand at 1900.538 kb on + strand at 1900.539 kb on - strand at 1900.539 kb on - strand at 1900.539 kb on - strand at 1900.539 kb on - strand at 1900.777 kb on + strand, within RALBFv3_RS08640 at 1900.778 kb on - strand, within RALBFv3_RS08640 at 1901.215 kb on + strand at 1901.215 kb on + strand at 1901.215 kb on + strand at 1901.216 kb on - strand at 1901.216 kb on - strand at 1901.216 kb on - strand at 1901.216 kb on - strand at 1901.233 kb on + strand at 1901.234 kb on - strand at 1901.320 kb on + strand, within RALBFv3_RS08645 at 1901.320 kb on + strand, within RALBFv3_RS08645 at 1901.321 kb on - strand, within RALBFv3_RS08645 at 1901.339 kb on - strand, within RALBFv3_RS08645 at 1901.380 kb on + strand, within RALBFv3_RS08645 at 1901.481 kb on + strand, within RALBFv3_RS08645
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,897,432 - -0.7 1,897,501 + -0.2 1,897,502 - +0.1 1,897,575 + RALBFv3_RS08630 0.12 -0.0 1,897,862 + +1.0 1,897,863 - -0.6 1,897,863 - +1.0 1,897,863 - +0.6 1,897,880 - -0.4 1,898,011 + +1.0 1,898,011 + -0.2 1,898,011 + -0.3 1,898,016 + +1.0 1,898,016 + +0.1 1,898,016 + +0.6 1,898,039 + +0.3 1,898,039 + -0.2 1,898,039 + -0.0 1,898,039 + -0.5 1,898,039 + -0.3 1,898,040 - -0.4 1,898,040 - -0.3 1,898,040 - -1.0 1,898,060 - +0.4 1,898,253 + -0.2 1,898,253 + -0.8 1,898,253 + +0.6 1,898,254 - +1.0 1,898,294 - +0.1 1,898,378 - -0.6 1,898,562 + -0.6 1,898,563 - -1.8 1,898,600 + RALBFv3_RS08635 0.11 +0.4 1,898,600 + RALBFv3_RS08635 0.11 +1.0 1,898,601 - RALBFv3_RS08635 0.11 +1.6 1,898,706 - RALBFv3_RS08635 0.16 -1.8 1,898,963 + RALBFv3_RS08635 0.28 +1.0 1,898,972 + RALBFv3_RS08635 0.28 -0.6 1,898,973 - RALBFv3_RS08635 0.28 +1.0 1,898,973 - RALBFv3_RS08635 0.28 -1.7 1,898,978 + RALBFv3_RS08635 0.28 +1.0 1,899,336 - RALBFv3_RS08635 0.45 -0.6 1,899,500 - RALBFv3_RS08635 0.53 -0.0 1,899,611 + RALBFv3_RS08635 0.58 -0.3 1,899,611 + RALBFv3_RS08635 0.58 +1.0 1,899,611 + RALBFv3_RS08635 0.58 -1.0 1,899,612 - RALBFv3_RS08635 0.58 +1.0 1,899,612 - RALBFv3_RS08635 0.58 -0.0 1,899,698 + RALBFv3_RS08635 0.62 -1.0 1,899,699 - RALBFv3_RS08635 0.62 -1.1 1,899,782 + RALBFv3_RS08635 0.66 +1.0 1,899,783 - RALBFv3_RS08635 0.66 -0.6 1,899,825 - RALBFv3_RS08635 0.68 -0.5 1,899,941 + RALBFv3_RS08635 0.74 -0.6 1,900,206 + RALBFv3_RS08635 0.86 +0.4 1,900,207 - RALBFv3_RS08635 0.86 -0.6 1,900,207 - RALBFv3_RS08635 0.86 -1.4 1,900,439 + -0.3 1,900,440 - +1.4 1,900,538 + -0.0 1,900,538 + -1.3 1,900,538 + +0.1 1,900,539 - -1.2 1,900,539 - +0.8 1,900,539 - -0.0 1,900,539 - +1.0 1,900,777 + RALBFv3_RS08640 0.12 -0.6 1,900,778 - RALBFv3_RS08640 0.12 -0.4 1,901,215 + +0.0 1,901,215 + -0.2 1,901,215 + +0.3 1,901,216 - -0.4 1,901,216 - +0.4 1,901,216 - -0.2 1,901,216 - -0.1 1,901,233 + +1.0 1,901,234 - +1.6 1,901,320 + RALBFv3_RS08645 0.10 +1.0 1,901,320 + RALBFv3_RS08645 0.10 -0.1 1,901,321 - RALBFv3_RS08645 0.11 -0.4 1,901,339 - RALBFv3_RS08645 0.12 -0.3 1,901,380 + RALBFv3_RS08645 0.16 -0.0 1,901,481 + RALBFv3_RS08645 0.26 -0.5
Or see this region's nucleotide sequence