Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS08565

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS08550 and RALBFv3_RS08555 are separated by 7 nucleotidesRALBFv3_RS08555 and RALBFv3_RS08560 are separated by 34 nucleotidesRALBFv3_RS08560 and RALBFv3_RS08565 overlap by 23 nucleotidesRALBFv3_RS08565 and RALBFv3_RS08570 are separated by 115 nucleotides RALBFv3_RS08550: RALBFv3_RS08550 - GntR family transcriptional regulator, at 1,881,798 to 1,882,487 _RS08550 RALBFv3_RS08555: RALBFv3_RS08555 - DUF1949 domain-containing protein, at 1,882,495 to 1,883,082 _RS08555 RALBFv3_RS08560: RALBFv3_RS08560 - XRE family transcriptional regulator, at 1,883,117 to 1,883,449 _RS08560 RALBFv3_RS08565: RALBFv3_RS08565 - type II toxin-antitoxin system RelE/ParE family toxin, at 1,883,427 to 1,883,699 _RS08565 RALBFv3_RS08570: RALBFv3_RS08570 - LysR family transcriptional regulator, at 1,883,815 to 1,884,726 _RS08570 Position (kb) 1883 1884Strain fitness (log2 ratio) -1 0 1at 1882.504 kb on + strandat 1882.504 kb on + strandat 1882.504 kb on + strandat 1882.505 kb on - strandat 1882.505 kb on - strandat 1882.780 kb on + strand, within RALBFv3_RS08555at 1882.780 kb on + strand, within RALBFv3_RS08555at 1882.781 kb on - strand, within RALBFv3_RS08555at 1882.910 kb on - strand, within RALBFv3_RS08555at 1882.954 kb on + strand, within RALBFv3_RS08555at 1882.954 kb on + strand, within RALBFv3_RS08555at 1882.954 kb on + strand, within RALBFv3_RS08555at 1882.955 kb on - strand, within RALBFv3_RS08555at 1883.364 kb on + strand, within RALBFv3_RS08560at 1883.364 kb on + strand, within RALBFv3_RS08560at 1883.365 kb on - strand, within RALBFv3_RS08560at 1883.428 kb on + strandat 1883.428 kb on + strandat 1883.428 kb on + strandat 1883.429 kb on - strandat 1883.429 kb on - strandat 1883.429 kb on - strandat 1883.429 kb on - strandat 1883.437 kb on + strandat 1883.438 kb on - strandat 1883.438 kb on - strandat 1883.475 kb on + strand, within RALBFv3_RS08565at 1883.685 kb on + strandat 1883.685 kb on + strandat 1883.686 kb on - strandat 1883.849 kb on + strandat 1883.850 kb on - strandat 1883.850 kb on - strandat 1883.850 kb on - strandat 1883.871 kb on + strandat 1883.931 kb on - strand, within RALBFv3_RS08570at 1884.120 kb on - strand, within RALBFv3_RS08570at 1884.215 kb on + strand, within RALBFv3_RS08570at 1884.312 kb on - strand, within RALBFv3_RS08570at 1884.312 kb on - strand, within RALBFv3_RS08570

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,882,504 + -0.2
1,882,504 + +1.0
1,882,504 + +0.0
1,882,505 - -0.4
1,882,505 - +1.0
1,882,780 + RALBFv3_RS08555 0.48 -0.3
1,882,780 + RALBFv3_RS08555 0.48 +1.2
1,882,781 - RALBFv3_RS08555 0.49 -0.5
1,882,910 - RALBFv3_RS08555 0.71 +1.0
1,882,954 + RALBFv3_RS08555 0.78 -0.3
1,882,954 + RALBFv3_RS08555 0.78 -0.2
1,882,954 + RALBFv3_RS08555 0.78 -0.0
1,882,955 - RALBFv3_RS08555 0.78 -0.2
1,883,364 + RALBFv3_RS08560 0.74 -0.2
1,883,364 + RALBFv3_RS08560 0.74 +0.1
1,883,365 - RALBFv3_RS08560 0.74 +0.6
1,883,428 + +1.0
1,883,428 + -0.5
1,883,428 + +0.2
1,883,429 - -0.0
1,883,429 - -0.3
1,883,429 - -1.6
1,883,429 - +0.0
1,883,437 + -0.3
1,883,438 - +1.0
1,883,438 - +1.0
1,883,475 + RALBFv3_RS08565 0.18 -0.7
1,883,685 + +0.6
1,883,685 + -0.3
1,883,686 - -0.7
1,883,849 + -0.2
1,883,850 - -0.0
1,883,850 - +0.1
1,883,850 - -0.0
1,883,871 + -0.7
1,883,931 - RALBFv3_RS08570 0.13 +0.1
1,884,120 - RALBFv3_RS08570 0.33 -0.2
1,884,215 + RALBFv3_RS08570 0.44 -0.3
1,884,312 - RALBFv3_RS08570 0.54 -0.2
1,884,312 - RALBFv3_RS08570 0.54 -0.6

Or see this region's nucleotide sequence