Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS07925 and RALBFv3_RS07930 are separated by 851 nucleotides RALBFv3_RS07930 and RALBFv3_RS07935 are separated by 214 nucleotides
RALBFv3_RS07925: RALBFv3_RS07925 - cytochrome c, at 1,746,495 to 1,747,358
_RS07925
RALBFv3_RS07930: RALBFv3_RS07930 - hypothetical protein, at 1,748,210 to 1,748,644
_RS07930
RALBFv3_RS07935: RALBFv3_RS07935 - hypothetical protein, at 1,748,859 to 1,749,359
_RS07935
Position (kb)
1748
1749 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1747.358 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.410 kb on + strand at 1747.411 kb on - strand at 1747.411 kb on - strand at 1747.411 kb on - strand at 1747.411 kb on - strand at 1747.411 kb on - strand at 1747.473 kb on + strand at 1747.474 kb on - strand at 1747.474 kb on - strand at 1747.474 kb on - strand at 1747.474 kb on - strand at 1747.474 kb on - strand at 1747.474 kb on - strand at 1747.474 kb on - strand at 1747.564 kb on + strand at 1747.565 kb on - strand at 1747.568 kb on + strand at 1747.568 kb on + strand at 1747.569 kb on - strand at 1747.579 kb on + strand at 1747.582 kb on + strand at 1747.582 kb on + strand at 1747.583 kb on - strand at 1747.583 kb on - strand at 1747.583 kb on - strand at 1747.583 kb on - strand at 1747.585 kb on + strand at 1747.585 kb on + strand at 1747.586 kb on - strand at 1747.586 kb on - strand at 1747.612 kb on + strand at 1747.660 kb on + strand at 1747.661 kb on - strand at 1747.811 kb on + strand at 1747.812 kb on - strand at 1747.890 kb on - strand at 1748.046 kb on - strand at 1748.046 kb on - strand at 1748.069 kb on + strand at 1748.069 kb on + strand at 1748.069 kb on + strand at 1748.069 kb on + strand at 1748.069 kb on + strand at 1748.135 kb on - strand at 1748.135 kb on - strand at 1748.135 kb on - strand at 1748.258 kb on - strand, within RALBFv3_RS07930 at 1748.258 kb on - strand, within RALBFv3_RS07930 at 1748.563 kb on + strand, within RALBFv3_RS07930 at 1748.954 kb on - strand, within RALBFv3_RS07935 at 1748.954 kb on - strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.057 kb on + strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.058 kb on - strand, within RALBFv3_RS07935 at 1749.200 kb on + strand, within RALBFv3_RS07935 at 1749.200 kb on + strand, within RALBFv3_RS07935 at 1749.201 kb on - strand, within RALBFv3_RS07935 at 1749.295 kb on - strand, within RALBFv3_RS07935 at 1749.310 kb on - strand at 1749.331 kb on + strand at 1749.331 kb on + strand at 1749.331 kb on + strand at 1749.331 kb on + strand at 1749.332 kb on - strand at 1749.332 kb on - strand at 1749.332 kb on - strand at 1749.383 kb on + strand at 1749.478 kb on + strand at 1749.478 kb on + strand at 1749.479 kb on - strand at 1749.586 kb on + strand at 1749.615 kb on + strand at 1749.618 kb on + strand at 1749.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,747,358 + +0.6 1,747,410 + -0.2 1,747,410 + +0.4 1,747,410 + +0.6 1,747,410 + -0.3 1,747,410 + +1.0 1,747,410 + +0.0 1,747,410 + +0.6 1,747,410 + +0.2 1,747,411 - +0.0 1,747,411 - -1.5 1,747,411 - +0.5 1,747,411 - +0.3 1,747,411 - -0.4 1,747,473 + +1.3 1,747,474 - +0.1 1,747,474 - +0.0 1,747,474 - -0.2 1,747,474 - +1.0 1,747,474 - -0.3 1,747,474 - +0.1 1,747,474 - +1.0 1,747,564 + -0.2 1,747,565 - -1.6 1,747,568 + -0.2 1,747,568 + +0.3 1,747,569 - +0.6 1,747,579 + -0.3 1,747,582 + -0.6 1,747,582 + -0.1 1,747,583 - +0.6 1,747,583 - +0.1 1,747,583 - +0.8 1,747,583 - -1.8 1,747,585 + +0.1 1,747,585 + -0.3 1,747,586 - +0.4 1,747,586 - -0.0 1,747,612 + +0.5 1,747,660 + +0.3 1,747,661 - -1.3 1,747,811 + +0.6 1,747,812 - -0.4 1,747,890 - -0.0 1,748,046 - -0.6 1,748,046 - -0.1 1,748,069 + +0.6 1,748,069 + -0.5 1,748,069 + -0.8 1,748,069 + +0.0 1,748,069 + -1.2 1,748,135 - -2.4 1,748,135 - -1.4 1,748,135 - +1.0 1,748,258 - RALBFv3_RS07930 0.11 +0.0 1,748,258 - RALBFv3_RS07930 0.11 -1.5 1,748,563 + RALBFv3_RS07930 0.81 +0.0 1,748,954 - RALBFv3_RS07935 0.19 +0.8 1,748,954 - RALBFv3_RS07935 0.19 -0.6 1,749,057 + RALBFv3_RS07935 0.40 -0.9 1,749,057 + RALBFv3_RS07935 0.40 -0.2 1,749,057 + RALBFv3_RS07935 0.40 -1.7 1,749,057 + RALBFv3_RS07935 0.40 -0.3 1,749,057 + RALBFv3_RS07935 0.40 -0.4 1,749,057 + RALBFv3_RS07935 0.40 +2.2 1,749,057 + RALBFv3_RS07935 0.40 -0.4 1,749,058 - RALBFv3_RS07935 0.40 -0.3 1,749,058 - RALBFv3_RS07935 0.40 -0.2 1,749,058 - RALBFv3_RS07935 0.40 -0.4 1,749,058 - RALBFv3_RS07935 0.40 +0.2 1,749,058 - RALBFv3_RS07935 0.40 -0.2 1,749,058 - RALBFv3_RS07935 0.40 -0.6 1,749,058 - RALBFv3_RS07935 0.40 -0.5 1,749,058 - RALBFv3_RS07935 0.40 +1.9 1,749,058 - RALBFv3_RS07935 0.40 -0.7 1,749,200 + RALBFv3_RS07935 0.68 -0.9 1,749,200 + RALBFv3_RS07935 0.68 -0.3 1,749,201 - RALBFv3_RS07935 0.68 -0.1 1,749,295 - RALBFv3_RS07935 0.87 -2.0 1,749,310 - +0.1 1,749,331 + +0.6 1,749,331 + +1.0 1,749,331 + -0.2 1,749,331 + +1.0 1,749,332 - -0.3 1,749,332 - -0.4 1,749,332 - +0.8 1,749,383 + -0.6 1,749,478 + +0.1 1,749,478 + -0.1 1,749,479 - -0.5 1,749,586 + -0.3 1,749,615 + +0.1 1,749,618 + -0.3 1,749,619 - -0.1
Or see this region's nucleotide sequence