Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS07735

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS07725 and RALBFv3_RS07730 are separated by 15 nucleotidesRALBFv3_RS07730 and RALBFv3_RS07735 are separated by 3 nucleotidesRALBFv3_RS07735 and RALBFv3_RS07740 are separated by 33 nucleotides RALBFv3_RS07725: RALBFv3_RS07725 - general secretion pathway protein GspL, at 1,693,391 to 1,694,830 _RS07725 RALBFv3_RS07730: RALBFv3_RS07730 - general secretion pathway protein GspM, at 1,694,846 to 1,695,424 _RS07730 RALBFv3_RS07735: RALBFv3_RS07735 - type II secretion system protein N, at 1,695,428 to 1,696,279 _RS07735 RALBFv3_RS07740: RALBFv3_RS07740 - type II secretion system protein GspD, at 1,696,313 to 1,698,742 _RS07740 Position (kb) 1695 1696 1697Strain fitness (log2 ratio) -1 0 1at 1694.667 kb on - strand, within RALBFv3_RS07725at 1694.716 kb on + strandat 1694.716 kb on + strandat 1694.716 kb on + strandat 1694.716 kb on + strandat 1694.716 kb on + strandat 1694.717 kb on - strandat 1694.846 kb on - strandat 1694.863 kb on + strandat 1694.864 kb on - strandat 1695.351 kb on - strand, within RALBFv3_RS07730at 1696.020 kb on + strand, within RALBFv3_RS07735at 1696.021 kb on - strand, within RALBFv3_RS07735at 1696.021 kb on - strand, within RALBFv3_RS07735at 1696.021 kb on - strand, within RALBFv3_RS07735at 1696.058 kb on + strand, within RALBFv3_RS07735at 1696.655 kb on + strand, within RALBFv3_RS07740at 1696.656 kb on - strand, within RALBFv3_RS07740at 1696.745 kb on - strand, within RALBFv3_RS07740at 1696.793 kb on + strand, within RALBFv3_RS07740at 1696.793 kb on + strand, within RALBFv3_RS07740at 1696.793 kb on + strand, within RALBFv3_RS07740at 1696.794 kb on - strand, within RALBFv3_RS07740at 1696.895 kb on + strand, within RALBFv3_RS07740

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,694,667 - RALBFv3_RS07725 0.89 -0.6
1,694,716 + +1.0
1,694,716 + -0.9
1,694,716 + +0.1
1,694,716 + +0.2
1,694,716 + +1.1
1,694,717 - +0.3
1,694,846 - +0.2
1,694,863 + +0.3
1,694,864 - +0.6
1,695,351 - RALBFv3_RS07730 0.87 +0.7
1,696,020 + RALBFv3_RS07735 0.69 -1.3
1,696,021 - RALBFv3_RS07735 0.70 -0.4
1,696,021 - RALBFv3_RS07735 0.70 -0.9
1,696,021 - RALBFv3_RS07735 0.70 -0.6
1,696,058 + RALBFv3_RS07735 0.74 -0.7
1,696,655 + RALBFv3_RS07740 0.14 -1.1
1,696,656 - RALBFv3_RS07740 0.14 +0.6
1,696,745 - RALBFv3_RS07740 0.18 -0.2
1,696,793 + RALBFv3_RS07740 0.20 +0.5
1,696,793 + RALBFv3_RS07740 0.20 -0.3
1,696,793 + RALBFv3_RS07740 0.20 +0.0
1,696,794 - RALBFv3_RS07740 0.20 -0.8
1,696,895 + RALBFv3_RS07740 0.24 -0.4

Or see this region's nucleotide sequence