Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS07425

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS07420 and RALBFv3_RS07425 are separated by 213 nucleotidesRALBFv3_RS07425 and RALBFv3_RS07430 are separated by 7 nucleotides RALBFv3_RS07420: RALBFv3_RS07420 - glucan biosynthesis protein D, at 1,631,565 to 1,633,172 _RS07420 RALBFv3_RS07425: RALBFv3_RS07425 - N-acylglucosamine 2-epimerase, at 1,633,386 to 1,634,636 _RS07425 RALBFv3_RS07430: RALBFv3_RS07430 - MFS transporter, at 1,634,644 to 1,635,933 _RS07430 Position (kb) 1633 1634 1635Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1632.453 kb on - strand, within RALBFv3_RS07420at 1632.514 kb on + strand, within RALBFv3_RS07420at 1632.664 kb on + strand, within RALBFv3_RS07420at 1632.665 kb on - strand, within RALBFv3_RS07420at 1632.665 kb on - strand, within RALBFv3_RS07420at 1632.949 kb on + strand, within RALBFv3_RS07420at 1633.006 kb on + strand, within RALBFv3_RS07420at 1633.007 kb on - strand, within RALBFv3_RS07420at 1633.007 kb on - strand, within RALBFv3_RS07420at 1633.097 kb on + strandat 1633.103 kb on + strandat 1633.103 kb on + strandat 1633.104 kb on - strandat 1633.104 kb on - strandat 1633.351 kb on + strandat 1633.352 kb on - strandat 1633.415 kb on - strandat 1633.537 kb on - strand, within RALBFv3_RS07425at 1633.620 kb on + strand, within RALBFv3_RS07425at 1633.791 kb on + strand, within RALBFv3_RS07425at 1633.792 kb on - strand, within RALBFv3_RS07425at 1633.792 kb on - strand, within RALBFv3_RS07425at 1634.144 kb on + strand, within RALBFv3_RS07425at 1634.205 kb on + strand, within RALBFv3_RS07425at 1634.308 kb on + strand, within RALBFv3_RS07425at 1634.309 kb on - strand, within RALBFv3_RS07425at 1634.398 kb on - strand, within RALBFv3_RS07425at 1634.556 kb on + strandat 1634.650 kb on - strandat 1634.719 kb on + strandat 1634.720 kb on - strandat 1634.734 kb on + strandat 1634.734 kb on + strandat 1634.740 kb on + strandat 1634.743 kb on + strandat 1634.744 kb on - strandat 1634.744 kb on - strandat 1634.869 kb on + strand, within RALBFv3_RS07430at 1635.397 kb on + strand, within RALBFv3_RS07430at 1635.412 kb on + strand, within RALBFv3_RS07430

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,632,453 - RALBFv3_RS07420 0.55 -0.1
1,632,514 + RALBFv3_RS07420 0.59 -1.1
1,632,664 + RALBFv3_RS07420 0.68 -0.5
1,632,665 - RALBFv3_RS07420 0.68 +0.5
1,632,665 - RALBFv3_RS07420 0.68 -0.1
1,632,949 + RALBFv3_RS07420 0.86 +0.1
1,633,006 + RALBFv3_RS07420 0.90 -0.2
1,633,007 - RALBFv3_RS07420 0.90 -0.3
1,633,007 - RALBFv3_RS07420 0.90 -0.3
1,633,097 + -0.4
1,633,103 + -0.4
1,633,103 + -0.3
1,633,104 - +1.1
1,633,104 - -0.4
1,633,351 + -0.2
1,633,352 - -2.5
1,633,415 - -2.5
1,633,537 - RALBFv3_RS07425 0.12 -4.2
1,633,620 + RALBFv3_RS07425 0.19 -3.6
1,633,791 + RALBFv3_RS07425 0.32 -2.1
1,633,792 - RALBFv3_RS07425 0.32 -2.1
1,633,792 - RALBFv3_RS07425 0.32 -1.3
1,634,144 + RALBFv3_RS07425 0.61 -3.6
1,634,205 + RALBFv3_RS07425 0.65 -2.3
1,634,308 + RALBFv3_RS07425 0.74 -1.5
1,634,309 - RALBFv3_RS07425 0.74 -1.5
1,634,398 - RALBFv3_RS07425 0.81 -3.7
1,634,556 + -3.1
1,634,650 - +0.1
1,634,719 + -0.5
1,634,720 - +0.0
1,634,734 + +0.4
1,634,734 + -0.6
1,634,740 + -0.5
1,634,743 + +0.1
1,634,744 - -0.1
1,634,744 - +0.1
1,634,869 + RALBFv3_RS07430 0.17 -0.2
1,635,397 + RALBFv3_RS07430 0.58 +0.2
1,635,412 + RALBFv3_RS07430 0.60 -0.5

Or see this region's nucleotide sequence