Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS07420 and RALBFv3_RS07425 are separated by 213 nucleotides RALBFv3_RS07425 and RALBFv3_RS07430 are separated by 7 nucleotides
RALBFv3_RS07420: RALBFv3_RS07420 - glucan biosynthesis protein D, at 1,631,565 to 1,633,172
_RS07420
RALBFv3_RS07425: RALBFv3_RS07425 - N-acylglucosamine 2-epimerase, at 1,633,386 to 1,634,636
_RS07425
RALBFv3_RS07430: RALBFv3_RS07430 - MFS transporter, at 1,634,644 to 1,635,933
_RS07430
Position (kb)
1633
1634
1635 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1632.453 kb on - strand, within RALBFv3_RS07420 at 1632.514 kb on + strand, within RALBFv3_RS07420 at 1632.664 kb on + strand, within RALBFv3_RS07420 at 1632.665 kb on - strand, within RALBFv3_RS07420 at 1632.665 kb on - strand, within RALBFv3_RS07420 at 1632.949 kb on + strand, within RALBFv3_RS07420 at 1633.006 kb on + strand, within RALBFv3_RS07420 at 1633.007 kb on - strand, within RALBFv3_RS07420 at 1633.007 kb on - strand, within RALBFv3_RS07420 at 1633.097 kb on + strand at 1633.103 kb on + strand at 1633.103 kb on + strand at 1633.104 kb on - strand at 1633.104 kb on - strand at 1633.351 kb on + strand at 1633.352 kb on - strand at 1633.415 kb on - strand at 1633.537 kb on - strand, within RALBFv3_RS07425 at 1633.620 kb on + strand, within RALBFv3_RS07425 at 1633.791 kb on + strand, within RALBFv3_RS07425 at 1633.792 kb on - strand, within RALBFv3_RS07425 at 1633.792 kb on - strand, within RALBFv3_RS07425 at 1634.144 kb on + strand, within RALBFv3_RS07425 at 1634.205 kb on + strand, within RALBFv3_RS07425 at 1634.308 kb on + strand, within RALBFv3_RS07425 at 1634.309 kb on - strand, within RALBFv3_RS07425 at 1634.398 kb on - strand, within RALBFv3_RS07425 at 1634.556 kb on + strand at 1634.650 kb on - strand at 1634.719 kb on + strand at 1634.720 kb on - strand at 1634.734 kb on + strand at 1634.734 kb on + strand at 1634.740 kb on + strand at 1634.743 kb on + strand at 1634.744 kb on - strand at 1634.744 kb on - strand at 1634.869 kb on + strand, within RALBFv3_RS07430 at 1635.397 kb on + strand, within RALBFv3_RS07430 at 1635.412 kb on + strand, within RALBFv3_RS07430
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,632,453 - RALBFv3_RS07420 0.55 -0.1 1,632,514 + RALBFv3_RS07420 0.59 -1.1 1,632,664 + RALBFv3_RS07420 0.68 -0.5 1,632,665 - RALBFv3_RS07420 0.68 +0.5 1,632,665 - RALBFv3_RS07420 0.68 -0.1 1,632,949 + RALBFv3_RS07420 0.86 +0.1 1,633,006 + RALBFv3_RS07420 0.90 -0.2 1,633,007 - RALBFv3_RS07420 0.90 -0.3 1,633,007 - RALBFv3_RS07420 0.90 -0.3 1,633,097 + -0.4 1,633,103 + -0.4 1,633,103 + -0.3 1,633,104 - +1.1 1,633,104 - -0.4 1,633,351 + -0.2 1,633,352 - -2.5 1,633,415 - -2.5 1,633,537 - RALBFv3_RS07425 0.12 -4.2 1,633,620 + RALBFv3_RS07425 0.19 -3.6 1,633,791 + RALBFv3_RS07425 0.32 -2.1 1,633,792 - RALBFv3_RS07425 0.32 -2.1 1,633,792 - RALBFv3_RS07425 0.32 -1.3 1,634,144 + RALBFv3_RS07425 0.61 -3.6 1,634,205 + RALBFv3_RS07425 0.65 -2.3 1,634,308 + RALBFv3_RS07425 0.74 -1.5 1,634,309 - RALBFv3_RS07425 0.74 -1.5 1,634,398 - RALBFv3_RS07425 0.81 -3.7 1,634,556 + -3.1 1,634,650 - +0.1 1,634,719 + -0.5 1,634,720 - +0.0 1,634,734 + +0.4 1,634,734 + -0.6 1,634,740 + -0.5 1,634,743 + +0.1 1,634,744 - -0.1 1,634,744 - +0.1 1,634,869 + RALBFv3_RS07430 0.17 -0.2 1,635,397 + RALBFv3_RS07430 0.58 +0.2 1,635,412 + RALBFv3_RS07430 0.60 -0.5
Or see this region's nucleotide sequence