Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS07050

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS07045 and RALBFv3_RS07050 are separated by 18 nucleotidesRALBFv3_RS07050 and RALBFv3_RS07055 are separated by 157 nucleotidesRALBFv3_RS07055 and RALBFv3_RS07060 are separated by 139 nucleotides RALBFv3_RS07045: RALBFv3_RS07045 - protein-disulfide reductase DsbD, at 1,562,044 to 1,563,882 _RS07045 RALBFv3_RS07050: RALBFv3_RS07050 - divalent-cation tolerance protein CutA, at 1,563,901 to 1,564,239 _RS07050 RALBFv3_RS07055: RALBFv3_RS07055 - 50S ribosomal protein L17, at 1,564,397 to 1,564,795 _RS07055 RALBFv3_RS07060: RALBFv3_RS07060 - DNA-directed RNA polymerase subunit alpha, at 1,564,935 to 1,565,915 _RS07060 Position (kb) 1563 1564 1565Strain fitness (log2 ratio) -1 0 1at 1562.920 kb on + strand, within RALBFv3_RS07045at 1563.029 kb on + strand, within RALBFv3_RS07045at 1563.029 kb on + strand, within RALBFv3_RS07045at 1563.030 kb on - strand, within RALBFv3_RS07045at 1563.030 kb on - strand, within RALBFv3_RS07045at 1563.156 kb on - strand, within RALBFv3_RS07045at 1563.156 kb on - strand, within RALBFv3_RS07045at 1563.179 kb on + strand, within RALBFv3_RS07045at 1563.179 kb on + strand, within RALBFv3_RS07045at 1563.459 kb on - strand, within RALBFv3_RS07045at 1563.558 kb on - strand, within RALBFv3_RS07045at 1563.671 kb on + strand, within RALBFv3_RS07045at 1563.677 kb on + strand, within RALBFv3_RS07045at 1563.678 kb on - strand, within RALBFv3_RS07045at 1563.945 kb on - strand, within RALBFv3_RS07050at 1564.034 kb on + strand, within RALBFv3_RS07050at 1564.324 kb on + strandat 1564.324 kb on + strandat 1564.324 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,562,920 + RALBFv3_RS07045 0.48 -0.3
1,563,029 + RALBFv3_RS07045 0.54 +0.1
1,563,029 + RALBFv3_RS07045 0.54 -0.7
1,563,030 - RALBFv3_RS07045 0.54 +0.6
1,563,030 - RALBFv3_RS07045 0.54 -0.8
1,563,156 - RALBFv3_RS07045 0.60 -0.5
1,563,156 - RALBFv3_RS07045 0.60 -1.7
1,563,179 + RALBFv3_RS07045 0.62 -0.1
1,563,179 + RALBFv3_RS07045 0.62 -0.4
1,563,459 - RALBFv3_RS07045 0.77 +0.4
1,563,558 - RALBFv3_RS07045 0.82 +0.6
1,563,671 + RALBFv3_RS07045 0.88 +1.1
1,563,677 + RALBFv3_RS07045 0.89 -0.2
1,563,678 - RALBFv3_RS07045 0.89 +0.5
1,563,945 - RALBFv3_RS07050 0.13 -0.7
1,564,034 + RALBFv3_RS07050 0.39 -0.7
1,564,324 + +0.3
1,564,324 + -0.3
1,564,324 + -0.8

Or see this region's nucleotide sequence