Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS07000 and RALBFv3_RS07005 are separated by 98 nucleotides RALBFv3_RS07005 and RALBFv3_RS07010 are separated by 8 nucleotides RALBFv3_RS07010 and RALBFv3_RS07015 are separated by 25 nucleotides
RALBFv3_RS07000: RALBFv3_RS07000 - protein-methionine-sulfoxide reductase catalytic subunit MsrP, at 1,552,716 to 1,553,711
_RS07000
RALBFv3_RS07005: RALBFv3_RS07005 - membrane protein, at 1,553,810 to 1,554,670
_RS07005
RALBFv3_RS07010: RALBFv3_RS07010 - RNA polymerase sigma factor, at 1,554,679 to 1,555,215
_RS07010
RALBFv3_RS07015: RALBFv3_RS07015 - ATP-binding protein, at 1,555,241 to 1,555,672
_RS07015
Position (kb) 1553
1554
1555 Strain fitness (log2 ratio) -2
-1
0
1 at 1553.191 kb on + strand, within RALBFv3_RS07000 at 1553.192 kb on - strand, within RALBFv3_RS07000 at 1553.341 kb on + strand, within RALBFv3_RS07000 at 1553.341 kb on + strand, within RALBFv3_RS07000 at 1553.411 kb on - strand, within RALBFv3_RS07000 at 1553.459 kb on - strand, within RALBFv3_RS07000 at 1553.503 kb on + strand, within RALBFv3_RS07000 at 1553.504 kb on - strand, within RALBFv3_RS07000 at 1553.844 kb on + strand at 1553.845 kb on - strand at 1553.934 kb on + strand, within RALBFv3_RS07005 at 1553.934 kb on + strand, within RALBFv3_RS07005 at 1554.247 kb on + strand, within RALBFv3_RS07005 at 1554.248 kb on - strand, within RALBFv3_RS07005 at 1554.248 kb on - strand, within RALBFv3_RS07005 at 1554.583 kb on - strand, within RALBFv3_RS07005 at 1555.320 kb on + strand, within RALBFv3_RS07015 at 1555.320 kb on + strand, within RALBFv3_RS07015 at 1555.320 kb on + strand, within RALBFv3_RS07015 at 1555.409 kb on - strand, within RALBFv3_RS07015 at 1555.489 kb on - strand, within RALBFv3_RS07015 at 1555.625 kb on + strand, within RALBFv3_RS07015 at 1555.626 kb on - strand, within RALBFv3_RS07015
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,553,191 + RALBFv3_RS07000 0.48 -0.3 1,553,192 - RALBFv3_RS07000 0.48 -0.1 1,553,341 + RALBFv3_RS07000 0.63 -0.4 1,553,341 + RALBFv3_RS07000 0.63 -1.3 1,553,411 - RALBFv3_RS07000 0.70 +0.1 1,553,459 - RALBFv3_RS07000 0.75 +1.1 1,553,503 + RALBFv3_RS07000 0.79 -0.2 1,553,504 - RALBFv3_RS07000 0.79 +1.1 1,553,844 + +0.1 1,553,845 - -0.8 1,553,934 + RALBFv3_RS07005 0.14 -1.3 1,553,934 + RALBFv3_RS07005 0.14 -0.7 1,554,247 + RALBFv3_RS07005 0.51 -1.7 1,554,248 - RALBFv3_RS07005 0.51 +0.1 1,554,248 - RALBFv3_RS07005 0.51 -2.5 1,554,583 - RALBFv3_RS07005 0.90 -1.7 1,555,320 + RALBFv3_RS07015 0.18 +1.1 1,555,320 + RALBFv3_RS07015 0.18 -0.0 1,555,320 + RALBFv3_RS07015 0.18 +1.1 1,555,409 - RALBFv3_RS07015 0.39 -0.2 1,555,489 - RALBFv3_RS07015 0.57 +0.9 1,555,625 + RALBFv3_RS07015 0.89 +0.1 1,555,626 - RALBFv3_RS07015 0.89 -0.3
Or see this region's nucleotide sequence