Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS06900

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS06890 and RALBFv3_RS06895 are separated by 28 nucleotidesRALBFv3_RS06895 and RALBFv3_RS06900 overlap by 4 nucleotidesRALBFv3_RS06900 and RALBFv3_RS06905 are separated by 236 nucleotides RALBFv3_RS06890: RALBFv3_RS06890 - outer membrane lipid asymmetry maintenance protein MlaD, at 1,526,130 to 1,526,648 _RS06890 RALBFv3_RS06895: RALBFv3_RS06895 - ABC transporter permease, at 1,526,677 to 1,527,444 _RS06895 RALBFv3_RS06900: RALBFv3_RS06900 - ABC transporter ATP-binding protein, at 1,527,441 to 1,528,280 _RS06900 RALBFv3_RS06905: RALBFv3_RS06905 - Plasmid pENH91 DNA, SacI-KpnI region containing chlorocatechol degradative operon, at 1,528,517 to 1,529,551 _RS06905 Position (kb) 1527 1528 1529Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1526.530 kb on + strand, within RALBFv3_RS06890at 1526.531 kb on - strand, within RALBFv3_RS06890at 1526.642 kb on + strandat 1527.506 kb on - strandat 1527.626 kb on - strand, within RALBFv3_RS06900at 1527.632 kb on - strand, within RALBFv3_RS06900at 1528.283 kb on + strandat 1528.338 kb on + strandat 1528.375 kb on + strandat 1528.882 kb on - strand, within RALBFv3_RS06905at 1529.173 kb on - strand, within RALBFv3_RS06905

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,526,530 + RALBFv3_RS06890 0.77 -3.3
1,526,531 - RALBFv3_RS06890 0.77 -4.1
1,526,642 + -5.3
1,527,506 - -3.6
1,527,626 - RALBFv3_RS06900 0.22 -1.7
1,527,632 - RALBFv3_RS06900 0.23 -2.8
1,528,283 + -0.3
1,528,338 + -4.1
1,528,375 + -3.8
1,528,882 - RALBFv3_RS06905 0.35 +0.2
1,529,173 - RALBFv3_RS06905 0.63 +0.0

Or see this region's nucleotide sequence