Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS06890 and RALBFv3_RS06895 are separated by 28 nucleotides RALBFv3_RS06895 and RALBFv3_RS06900 overlap by 4 nucleotides RALBFv3_RS06900 and RALBFv3_RS06905 are separated by 236 nucleotides
RALBFv3_RS06890: RALBFv3_RS06890 - outer membrane lipid asymmetry maintenance protein MlaD, at 1,526,130 to 1,526,648
_RS06890
RALBFv3_RS06895: RALBFv3_RS06895 - ABC transporter permease, at 1,526,677 to 1,527,444
_RS06895
RALBFv3_RS06900: RALBFv3_RS06900 - ABC transporter ATP-binding protein, at 1,527,441 to 1,528,280
_RS06900
RALBFv3_RS06905: RALBFv3_RS06905 - Plasmid pENH91 DNA, SacI-KpnI region containing chlorocatechol degradative operon, at 1,528,517 to 1,529,551
_RS06905
Position (kb)
1527
1528
1529 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1526.530 kb on + strand, within RALBFv3_RS06890 at 1526.531 kb on - strand, within RALBFv3_RS06890 at 1526.642 kb on + strand at 1527.506 kb on - strand at 1527.626 kb on - strand, within RALBFv3_RS06900 at 1527.632 kb on - strand, within RALBFv3_RS06900 at 1528.283 kb on + strand at 1528.338 kb on + strand at 1528.375 kb on + strand at 1528.882 kb on - strand, within RALBFv3_RS06905 at 1529.173 kb on - strand, within RALBFv3_RS06905
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,526,530 + RALBFv3_RS06890 0.77 -3.3 1,526,531 - RALBFv3_RS06890 0.77 -4.1 1,526,642 + -5.3 1,527,506 - -3.6 1,527,626 - RALBFv3_RS06900 0.22 -1.7 1,527,632 - RALBFv3_RS06900 0.23 -2.8 1,528,283 + -0.3 1,528,338 + -4.1 1,528,375 + -3.8 1,528,882 - RALBFv3_RS06905 0.35 +0.2 1,529,173 - RALBFv3_RS06905 0.63 +0.0
Or see this region's nucleotide sequence