Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS06880 and RALBFv3_RS06885 are separated by 154 nucleotides RALBFv3_RS06885 and RALBFv3_RS06890 are separated by 3 nucleotides RALBFv3_RS06890 and RALBFv3_RS06895 are separated by 28 nucleotides RALBFv3_RS06895 and RALBFv3_RS06900 overlap by 4 nucleotides
RALBFv3_RS06880: RALBFv3_RS06880 - hypothetical protein, at 1,524,527 to 1,525,162
_RS06880
RALBFv3_RS06885: RALBFv3_RS06885 - ABC transporter, at 1,525,317 to 1,526,126
_RS06885
RALBFv3_RS06890: RALBFv3_RS06890 - outer membrane lipid asymmetry maintenance protein MlaD, at 1,526,130 to 1,526,648
_RS06890
RALBFv3_RS06895: RALBFv3_RS06895 - ABC transporter permease, at 1,526,677 to 1,527,444
_RS06895
RALBFv3_RS06900: RALBFv3_RS06900 - ABC transporter ATP-binding protein, at 1,527,441 to 1,528,280
_RS06900
Position (kb) 1526
1527 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1525.163 kb on - strand at 1525.275 kb on - strand at 1525.305 kb on - strand at 1525.484 kb on - strand, within RALBFv3_RS06885 at 1525.484 kb on - strand, within RALBFv3_RS06885 at 1525.594 kb on + strand, within RALBFv3_RS06885 at 1525.678 kb on + strand, within RALBFv3_RS06885 at 1525.678 kb on + strand, within RALBFv3_RS06885 at 1525.933 kb on + strand, within RALBFv3_RS06885 at 1525.933 kb on + strand, within RALBFv3_RS06885 at 1525.934 kb on - strand, within RALBFv3_RS06885 at 1526.131 kb on + strand at 1526.131 kb on + strand at 1526.213 kb on - strand, within RALBFv3_RS06890 at 1526.321 kb on - strand, within RALBFv3_RS06890 at 1526.530 kb on + strand, within RALBFv3_RS06890 at 1526.531 kb on - strand, within RALBFv3_RS06890 at 1526.642 kb on + strand at 1527.506 kb on - strand at 1527.626 kb on - strand, within RALBFv3_RS06900 at 1527.632 kb on - strand, within RALBFv3_RS06900
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,525,163 - +1.1 1,525,275 - +0.3 1,525,305 - -0.1 1,525,484 - RALBFv3_RS06885 0.21 -4.2 1,525,484 - RALBFv3_RS06885 0.21 -2.7 1,525,594 + RALBFv3_RS06885 0.34 -2.8 1,525,678 + RALBFv3_RS06885 0.45 -4.5 1,525,678 + RALBFv3_RS06885 0.45 -4.0 1,525,933 + RALBFv3_RS06885 0.76 -2.5 1,525,933 + RALBFv3_RS06885 0.76 -2.1 1,525,934 - RALBFv3_RS06885 0.76 -4.5 1,526,131 + +0.1 1,526,131 + -0.4 1,526,213 - RALBFv3_RS06890 0.16 -1.7 1,526,321 - RALBFv3_RS06890 0.37 -2.1 1,526,530 + RALBFv3_RS06890 0.77 -3.3 1,526,531 - RALBFv3_RS06890 0.77 -4.1 1,526,642 + -5.3 1,527,506 - -3.6 1,527,626 - RALBFv3_RS06900 0.22 -1.7 1,527,632 - RALBFv3_RS06900 0.23 -2.8
Or see this region's nucleotide sequence