Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS06780 and RALBFv3_RS06785 are separated by 70 nucleotides RALBFv3_RS06785 and RALBFv3_RS06790 are separated by 52 nucleotides RALBFv3_RS06790 and RALBFv3_RS06795 are separated by 19 nucleotides RALBFv3_RS06795 and RALBFv3_RS06800 are separated by 62 nucleotides
RALBFv3_RS06780: RALBFv3_RS06780 - ABC transporter ATP-binding protein, at 1,509,693 to 1,510,769
_RS06780
RALBFv3_RS06785: RALBFv3_RS06785 - twin-arginine translocase subunit TatC, at 1,510,840 to 1,511,637
_RS06785
RALBFv3_RS06790: RALBFv3_RS06790 - twin-arginine translocase subunit TatB, at 1,511,690 to 1,512,208
_RS06790
RALBFv3_RS06795: RALBFv3_RS06795 - twin-arginine translocase subunit TatA, at 1,512,228 to 1,512,485
_RS06795
RALBFv3_RS06800: RALBFv3_RS06800 - membrane protein, at 1,512,548 to 1,512,922
_RS06800
Position (kb)
1510
1511
1512 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1509.960 kb on + strand, within RALBFv3_RS06780 at 1510.158 kb on + strand, within RALBFv3_RS06780 at 1510.158 kb on + strand, within RALBFv3_RS06780 at 1510.159 kb on - strand, within RALBFv3_RS06780 at 1510.593 kb on - strand, within RALBFv3_RS06780 at 1510.722 kb on + strand at 1510.723 kb on - strand at 1510.767 kb on + strand at 1510.926 kb on - strand, within RALBFv3_RS06785 at 1511.187 kb on - strand, within RALBFv3_RS06785 at 1511.261 kb on + strand, within RALBFv3_RS06785 at 1511.262 kb on - strand, within RALBFv3_RS06785 at 1511.262 kb on - strand, within RALBFv3_RS06785 at 1511.339 kb on + strand, within RALBFv3_RS06785 at 1511.498 kb on + strand, within RALBFv3_RS06785 at 1512.500 kb on + strand at 1512.504 kb on + strand at 1512.537 kb on - strand at 1512.562 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,509,960 + RALBFv3_RS06780 0.25 +1.1 1,510,158 + RALBFv3_RS06780 0.43 -1.1 1,510,158 + RALBFv3_RS06780 0.43 +0.2 1,510,159 - RALBFv3_RS06780 0.43 -0.4 1,510,593 - RALBFv3_RS06780 0.84 -0.7 1,510,722 + -0.7 1,510,723 - -0.1 1,510,767 + -1.4 1,510,926 - RALBFv3_RS06785 0.11 -4.3 1,511,187 - RALBFv3_RS06785 0.43 +0.1 1,511,261 + RALBFv3_RS06785 0.53 -0.5 1,511,262 - RALBFv3_RS06785 0.53 +0.1 1,511,262 - RALBFv3_RS06785 0.53 -1.5 1,511,339 + RALBFv3_RS06785 0.63 -2.6 1,511,498 + RALBFv3_RS06785 0.82 +0.1 1,512,500 + -0.9 1,512,504 + -0.5 1,512,537 - +0.1 1,512,562 - -0.2
Or see this region's nucleotide sequence