Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS06610

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS06600 and RALBFv3_RS06605 are separated by 20 nucleotidesRALBFv3_RS06605 and RALBFv3_RS06610 are separated by 312 nucleotidesRALBFv3_RS06610 and RALBFv3_RS06615 are separated by 346 nucleotides RALBFv3_RS06600: RALBFv3_RS06600 - monothiol glutaredoxin, Grx4 family, at 1,467,923 to 1,468,234 _RS06600 RALBFv3_RS06605: RALBFv3_RS06605 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 1,468,255 to 1,468,899 _RS06605 RALBFv3_RS06610: RALBFv3_RS06610 - hypothetical protein, at 1,469,212 to 1,469,931 _RS06610 RALBFv3_RS06615: RALBFv3_RS06615 - glucan biosynthesis protein D, at 1,470,278 to 1,471,804 _RS06615 Position (kb) 1469 1470Strain fitness (log2 ratio) -3 -2 -1 0 1at 1468.885 kb on + strandat 1468.885 kb on + strandat 1468.939 kb on + strandat 1468.940 kb on - strandat 1469.035 kb on + strandat 1469.036 kb on - strandat 1469.133 kb on + strandat 1469.133 kb on + strandat 1469.135 kb on + strandat 1469.135 kb on + strandat 1469.135 kb on + strandat 1469.136 kb on - strandat 1469.136 kb on - strandat 1469.136 kb on - strandat 1469.171 kb on + strandat 1469.171 kb on + strandat 1469.181 kb on - strandat 1469.181 kb on - strandat 1469.207 kb on + strandat 1469.208 kb on - strandat 1469.399 kb on + strand, within RALBFv3_RS06610at 1469.399 kb on + strand, within RALBFv3_RS06610at 1469.400 kb on - strand, within RALBFv3_RS06610at 1469.400 kb on - strand, within RALBFv3_RS06610at 1469.400 kb on - strand, within RALBFv3_RS06610at 1469.747 kb on - strand, within RALBFv3_RS06610at 1469.747 kb on - strand, within RALBFv3_RS06610at 1469.747 kb on - strand, within RALBFv3_RS06610at 1470.412 kb on + strandat 1470.707 kb on + strand, within RALBFv3_RS06615at 1470.707 kb on + strand, within RALBFv3_RS06615at 1470.773 kb on + strand, within RALBFv3_RS06615at 1470.893 kb on + strand, within RALBFv3_RS06615

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,468,885 + +0.3
1,468,885 + +1.1
1,468,939 + +0.6
1,468,940 - +0.1
1,469,035 + -0.2
1,469,036 - -0.5
1,469,133 + -1.2
1,469,133 + +0.8
1,469,135 + +0.3
1,469,135 + -0.0
1,469,135 + -0.2
1,469,136 - -0.6
1,469,136 - +0.1
1,469,136 - -0.4
1,469,171 + +0.5
1,469,171 + -0.3
1,469,181 - +0.0
1,469,181 - -0.1
1,469,207 + -0.9
1,469,208 - -0.3
1,469,399 + RALBFv3_RS06610 0.26 -2.1
1,469,399 + RALBFv3_RS06610 0.26 -0.8
1,469,400 - RALBFv3_RS06610 0.26 -0.8
1,469,400 - RALBFv3_RS06610 0.26 -3.3
1,469,400 - RALBFv3_RS06610 0.26 -1.3
1,469,747 - RALBFv3_RS06610 0.74 -1.5
1,469,747 - RALBFv3_RS06610 0.74 -1.7
1,469,747 - RALBFv3_RS06610 0.74 -1.7
1,470,412 + +0.1
1,470,707 + RALBFv3_RS06615 0.28 -0.5
1,470,707 + RALBFv3_RS06615 0.28 +1.1
1,470,773 + RALBFv3_RS06615 0.32 -0.9
1,470,893 + RALBFv3_RS06615 0.40 -0.9

Or see this region's nucleotide sequence