Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS06600 and RALBFv3_RS06605 are separated by 20 nucleotides RALBFv3_RS06605 and RALBFv3_RS06610 are separated by 312 nucleotides RALBFv3_RS06610 and RALBFv3_RS06615 are separated by 346 nucleotides
RALBFv3_RS06600: RALBFv3_RS06600 - monothiol glutaredoxin, Grx4 family, at 1,467,923 to 1,468,234
_RS06600
RALBFv3_RS06605: RALBFv3_RS06605 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 1,468,255 to 1,468,899
_RS06605
RALBFv3_RS06610: RALBFv3_RS06610 - hypothetical protein, at 1,469,212 to 1,469,931
_RS06610
RALBFv3_RS06615: RALBFv3_RS06615 - glucan biosynthesis protein D, at 1,470,278 to 1,471,804
_RS06615
Position (kb)
1469
1470 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1468.885 kb on + strand at 1468.885 kb on + strand at 1468.939 kb on + strand at 1468.940 kb on - strand at 1469.035 kb on + strand at 1469.036 kb on - strand at 1469.133 kb on + strand at 1469.133 kb on + strand at 1469.135 kb on + strand at 1469.135 kb on + strand at 1469.135 kb on + strand at 1469.136 kb on - strand at 1469.136 kb on - strand at 1469.136 kb on - strand at 1469.171 kb on + strand at 1469.171 kb on + strand at 1469.181 kb on - strand at 1469.181 kb on - strand at 1469.207 kb on + strand at 1469.208 kb on - strand at 1469.399 kb on + strand, within RALBFv3_RS06610 at 1469.399 kb on + strand, within RALBFv3_RS06610 at 1469.400 kb on - strand, within RALBFv3_RS06610 at 1469.400 kb on - strand, within RALBFv3_RS06610 at 1469.400 kb on - strand, within RALBFv3_RS06610 at 1469.747 kb on - strand, within RALBFv3_RS06610 at 1469.747 kb on - strand, within RALBFv3_RS06610 at 1469.747 kb on - strand, within RALBFv3_RS06610 at 1470.412 kb on + strand at 1470.707 kb on + strand, within RALBFv3_RS06615 at 1470.707 kb on + strand, within RALBFv3_RS06615 at 1470.773 kb on + strand, within RALBFv3_RS06615 at 1470.893 kb on + strand, within RALBFv3_RS06615
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,468,885 + +0.3 1,468,885 + +1.1 1,468,939 + +0.6 1,468,940 - +0.1 1,469,035 + -0.2 1,469,036 - -0.5 1,469,133 + -1.2 1,469,133 + +0.8 1,469,135 + +0.3 1,469,135 + -0.0 1,469,135 + -0.2 1,469,136 - -0.6 1,469,136 - +0.1 1,469,136 - -0.4 1,469,171 + +0.5 1,469,171 + -0.3 1,469,181 - +0.0 1,469,181 - -0.1 1,469,207 + -0.9 1,469,208 - -0.3 1,469,399 + RALBFv3_RS06610 0.26 -2.1 1,469,399 + RALBFv3_RS06610 0.26 -0.8 1,469,400 - RALBFv3_RS06610 0.26 -0.8 1,469,400 - RALBFv3_RS06610 0.26 -3.3 1,469,400 - RALBFv3_RS06610 0.26 -1.3 1,469,747 - RALBFv3_RS06610 0.74 -1.5 1,469,747 - RALBFv3_RS06610 0.74 -1.7 1,469,747 - RALBFv3_RS06610 0.74 -1.7 1,470,412 + +0.1 1,470,707 + RALBFv3_RS06615 0.28 -0.5 1,470,707 + RALBFv3_RS06615 0.28 +1.1 1,470,773 + RALBFv3_RS06615 0.32 -0.9 1,470,893 + RALBFv3_RS06615 0.40 -0.9
Or see this region's nucleotide sequence