Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS06480 and RALBFv3_RS06485 are separated by 377 nucleotides RALBFv3_RS06485 and RALBFv3_RS06490 are separated by 17 nucleotides RALBFv3_RS06490 and RALBFv3_RS06495 overlap by 1 nucleotides
RALBFv3_RS06480: RALBFv3_RS06480 - phosphoglycolate phosphatase, at 1,444,275 to 1,445,015
_RS06480
RALBFv3_RS06485: RALBFv3_RS06485 - anthranilate synthase component I, at 1,445,393 to 1,446,928
_RS06485
RALBFv3_RS06490: RALBFv3_RS06490 - type 1 glutamine amidotransferase, at 1,446,946 to 1,447,515
_RS06490
RALBFv3_RS06495: RALBFv3_RS06495 - DUF1311 domain-containing protein, at 1,447,515 to 1,447,952
_RS06495
Position (kb) 1445
1446
1447 Strain fitness (log2 ratio) -1
0
1 at 1444.431 kb on + strand, within RALBFv3_RS06480 at 1444.432 kb on - strand, within RALBFv3_RS06480 at 1444.432 kb on - strand, within RALBFv3_RS06480 at 1444.542 kb on + strand, within RALBFv3_RS06480 at 1444.542 kb on + strand, within RALBFv3_RS06480 at 1444.575 kb on + strand, within RALBFv3_RS06480 at 1444.575 kb on + strand, within RALBFv3_RS06480 at 1444.879 kb on - strand, within RALBFv3_RS06480 at 1444.879 kb on - strand, within RALBFv3_RS06480 at 1444.879 kb on - strand, within RALBFv3_RS06480 at 1444.898 kb on - strand, within RALBFv3_RS06480 at 1444.922 kb on - strand, within RALBFv3_RS06480 at 1445.576 kb on + strand, within RALBFv3_RS06485 at 1446.062 kb on + strand, within RALBFv3_RS06485 at 1446.063 kb on - strand, within RALBFv3_RS06485 at 1446.206 kb on + strand, within RALBFv3_RS06485 at 1446.213 kb on - strand, within RALBFv3_RS06485 at 1446.213 kb on - strand, within RALBFv3_RS06485 at 1446.998 kb on - strand at 1447.027 kb on - strand, within RALBFv3_RS06490
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,444,431 + RALBFv3_RS06480 0.21 +0.1 1,444,432 - RALBFv3_RS06480 0.21 +0.1 1,444,432 - RALBFv3_RS06480 0.21 +0.5 1,444,542 + RALBFv3_RS06480 0.36 -0.2 1,444,542 + RALBFv3_RS06480 0.36 -1.1 1,444,575 + RALBFv3_RS06480 0.40 -0.8 1,444,575 + RALBFv3_RS06480 0.40 -0.0 1,444,879 - RALBFv3_RS06480 0.82 +0.1 1,444,879 - RALBFv3_RS06480 0.82 +1.7 1,444,879 - RALBFv3_RS06480 0.82 -0.3 1,444,898 - RALBFv3_RS06480 0.84 +0.1 1,444,922 - RALBFv3_RS06480 0.87 -0.5 1,445,576 + RALBFv3_RS06485 0.12 -0.5 1,446,062 + RALBFv3_RS06485 0.44 +0.1 1,446,063 - RALBFv3_RS06485 0.44 +1.1 1,446,206 + RALBFv3_RS06485 0.53 -0.9 1,446,213 - RALBFv3_RS06485 0.53 +1.1 1,446,213 - RALBFv3_RS06485 0.53 -0.9 1,446,998 - -0.5 1,447,027 - RALBFv3_RS06490 0.14 +0.1
Or see this region's nucleotide sequence