Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS06085

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntRALBFv3_RS06080 and RALBFv3_RS06085 overlap by 8 nucleotidesRALBFv3_RS06085 and RALBFv3_RS06090 are separated by 26 nucleotides RALBFv3_RS06080: RALBFv3_RS06080 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 1,354,179 to 1,354,781 _RS06080 RALBFv3_RS06085: RALBFv3_RS06085 - sigma-54-dependent Fis family transcriptional regulator, at 1,354,774 to 1,356,459 _RS06085 RALBFv3_RS06090: RALBFv3_RS06090 - PAS domain-containing sensor histidine kinase, at 1,356,486 to 1,358,549 _RS06090 Position (kb) 1354 1355 1356 1357Strain fitness (log2 ratio) -2 -1 0 1at 1354.138 kb on - strandat 1354.205 kb on - strandat 1355.091 kb on - strand, within RALBFv3_RS06085at 1355.213 kb on - strand, within RALBFv3_RS06085at 1355.620 kb on + strand, within RALBFv3_RS06085at 1355.620 kb on + strand, within RALBFv3_RS06085at 1355.621 kb on - strand, within RALBFv3_RS06085at 1355.808 kb on - strand, within RALBFv3_RS06085at 1356.142 kb on - strand, within RALBFv3_RS06085at 1356.144 kb on - strand, within RALBFv3_RS06085at 1356.144 kb on - strand, within RALBFv3_RS06085at 1356.195 kb on - strand, within RALBFv3_RS06085at 1356.661 kb on + strandat 1356.661 kb on + strandat 1356.790 kb on + strand, within RALBFv3_RS06090at 1356.829 kb on + strand, within RALBFv3_RS06090

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
remove
1,354,138 - -0.5
1,354,205 - +0.3
1,355,091 - RALBFv3_RS06085 0.19 +1.1
1,355,213 - RALBFv3_RS06085 0.26 -1.9
1,355,620 + RALBFv3_RS06085 0.50 -1.2
1,355,620 + RALBFv3_RS06085 0.50 -0.8
1,355,621 - RALBFv3_RS06085 0.50 -1.1
1,355,808 - RALBFv3_RS06085 0.61 -0.4
1,356,142 - RALBFv3_RS06085 0.81 -0.9
1,356,144 - RALBFv3_RS06085 0.81 -0.6
1,356,144 - RALBFv3_RS06085 0.81 -1.8
1,356,195 - RALBFv3_RS06085 0.84 -0.9
1,356,661 + -0.6
1,356,661 + +0.1
1,356,790 + RALBFv3_RS06090 0.15 +0.1
1,356,829 + RALBFv3_RS06090 0.17 +0.4

Or see this region's nucleotide sequence