Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS06020

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS06015 and RALBFv3_RS06020 are separated by 9 nucleotidesRALBFv3_RS06020 and RALBFv3_RS23710 are separated by 249 nucleotidesRALBFv3_RS23710 and RALBFv3_RS23715 are separated by 29 nucleotides RALBFv3_RS06015: RALBFv3_RS06015 - carbohydrate kinase family protein, at 1,336,931 to 1,337,866 _RS06015 RALBFv3_RS06020: RALBFv3_RS06020 - membrane protein, at 1,337,876 to 1,338,343 _RS06020 RALBFv3_RS23710: RALBFv3_RS23710 - histone, at 1,338,593 to 1,339,180 _RS23710 RALBFv3_RS23715: RALBFv3_RS23715 - hypothetical protein, at 1,339,210 to 1,339,656 _RS23715 Position (kb) 1337 1338 1339Strain fitness (log2 ratio) -1 0 1 2at 1336.913 kb on + strandat 1337.160 kb on - strand, within RALBFv3_RS06015at 1337.168 kb on + strand, within RALBFv3_RS06015at 1337.630 kb on + strand, within RALBFv3_RS06015at 1337.631 kb on - strand, within RALBFv3_RS06015at 1337.631 kb on - strand, within RALBFv3_RS06015at 1337.635 kb on + strand, within RALBFv3_RS06015at 1337.858 kb on + strandat 1337.969 kb on + strand, within RALBFv3_RS06020at 1337.970 kb on - strand, within RALBFv3_RS06020at 1338.386 kb on + strandat 1339.039 kb on + strand, within RALBFv3_RS23710at 1339.040 kb on - strand, within RALBFv3_RS23710at 1339.069 kb on + strand, within RALBFv3_RS23710at 1339.166 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,336,913 + -0.4
1,337,160 - RALBFv3_RS06015 0.24 +1.1
1,337,168 + RALBFv3_RS06015 0.25 -1.0
1,337,630 + RALBFv3_RS06015 0.75 -0.9
1,337,631 - RALBFv3_RS06015 0.75 +0.5
1,337,631 - RALBFv3_RS06015 0.75 +2.1
1,337,635 + RALBFv3_RS06015 0.75 -0.9
1,337,858 + +0.8
1,337,969 + RALBFv3_RS06020 0.20 -0.8
1,337,970 - RALBFv3_RS06020 0.20 -0.3
1,338,386 + -0.4
1,339,039 + RALBFv3_RS23710 0.76 +0.1
1,339,040 - RALBFv3_RS23710 0.76 -0.4
1,339,069 + RALBFv3_RS23710 0.81 -0.4
1,339,166 - -0.1

Or see this region's nucleotide sequence