Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS06005 and RALBFv3_RS06010 are separated by 36 nucleotides RALBFv3_RS06010 and RALBFv3_RS06015 are separated by 85 nucleotides RALBFv3_RS06015 and RALBFv3_RS06020 are separated by 9 nucleotides RALBFv3_RS06020 and RALBFv3_RS23710 are separated by 249 nucleotides
RALBFv3_RS06005: RALBFv3_RS06005 - DUF3426 domain-containing protein, at 1,334,887 to 1,336,308
_RS06005
RALBFv3_RS06010: RALBFv3_RS06010 - thiol peroxidase, at 1,336,345 to 1,336,845
_RS06010
RALBFv3_RS06015: RALBFv3_RS06015 - carbohydrate kinase family protein, at 1,336,931 to 1,337,866
_RS06015
RALBFv3_RS06020: RALBFv3_RS06020 - membrane protein, at 1,337,876 to 1,338,343
_RS06020
RALBFv3_RS23710: RALBFv3_RS23710 - histone, at 1,338,593 to 1,339,180
_RS23710
Position (kb) 1336
1337
1338 Strain fitness (log2 ratio) -1
0
1
2 at 1335.977 kb on + strand, within RALBFv3_RS06005 at 1336.057 kb on + strand, within RALBFv3_RS06005 at 1336.057 kb on + strand, within RALBFv3_RS06005 at 1336.057 kb on + strand, within RALBFv3_RS06005 at 1336.058 kb on - strand, within RALBFv3_RS06005 at 1336.180 kb on + strand at 1336.205 kb on - strand at 1336.300 kb on + strand at 1336.326 kb on + strand at 1336.326 kb on + strand at 1336.327 kb on - strand at 1336.327 kb on - strand at 1336.327 kb on - strand at 1336.579 kb on - strand, within RALBFv3_RS06010 at 1336.693 kb on + strand, within RALBFv3_RS06010 at 1336.843 kb on + strand at 1336.843 kb on + strand at 1336.913 kb on + strand at 1337.160 kb on - strand, within RALBFv3_RS06015 at 1337.168 kb on + strand, within RALBFv3_RS06015 at 1337.630 kb on + strand, within RALBFv3_RS06015 at 1337.631 kb on - strand, within RALBFv3_RS06015 at 1337.631 kb on - strand, within RALBFv3_RS06015 at 1337.635 kb on + strand, within RALBFv3_RS06015 at 1337.858 kb on + strand at 1337.969 kb on + strand, within RALBFv3_RS06020 at 1337.970 kb on - strand, within RALBFv3_RS06020 at 1338.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,335,977 + RALBFv3_RS06005 0.77 +0.1 1,336,057 + RALBFv3_RS06005 0.82 +0.4 1,336,057 + RALBFv3_RS06005 0.82 -0.5 1,336,057 + RALBFv3_RS06005 0.82 +0.2 1,336,058 - RALBFv3_RS06005 0.82 +1.1 1,336,180 + +1.1 1,336,205 - +0.4 1,336,300 + +0.1 1,336,326 + +0.6 1,336,326 + +0.1 1,336,327 - +0.8 1,336,327 - -0.3 1,336,327 - -0.2 1,336,579 - RALBFv3_RS06010 0.47 +0.5 1,336,693 + RALBFv3_RS06010 0.69 -0.2 1,336,843 + +0.0 1,336,843 + -0.1 1,336,913 + -0.4 1,337,160 - RALBFv3_RS06015 0.24 +1.1 1,337,168 + RALBFv3_RS06015 0.25 -1.0 1,337,630 + RALBFv3_RS06015 0.75 -0.9 1,337,631 - RALBFv3_RS06015 0.75 +0.5 1,337,631 - RALBFv3_RS06015 0.75 +2.1 1,337,635 + RALBFv3_RS06015 0.75 -0.9 1,337,858 + +0.8 1,337,969 + RALBFv3_RS06020 0.20 -0.8 1,337,970 - RALBFv3_RS06020 0.20 -0.3 1,338,386 + -0.4
Or see this region's nucleotide sequence