Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05960 and RALBFv3_RS05965 overlap by 4 nucleotides RALBFv3_RS05965 and RALBFv3_RS05970 are separated by 6 nucleotides RALBFv3_RS05970 and RALBFv3_RS05975 are separated by 118 nucleotides RALBFv3_RS05975 and RALBFv3_RS05980 are separated by 75 nucleotides
RALBFv3_RS05960: RALBFv3_RS05960 - chorismate lyase, at 1,327,127 to 1,327,714
_RS05960
RALBFv3_RS05965: RALBFv3_RS05965 - esterase, at 1,327,711 to 1,328,343
_RS05965
RALBFv3_RS05970: RALBFv3_RS05970 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 1,328,350 to 1,329,735
_RS05970
RALBFv3_RS05975: RALBFv3_RS05975 - hypothetical protein, at 1,329,854 to 1,330,528
_RS05975
RALBFv3_RS05980: RALBFv3_RS05980 - TlpA family protein disulfide reductase, at 1,330,604 to 1,331,128
_RS05980
Position (kb) 1328
1329
1330 Strain fitness (log2 ratio) -2
-1
0
1 at 1327.506 kb on - strand, within RALBFv3_RS05960 at 1327.648 kb on - strand, within RALBFv3_RS05960 at 1327.794 kb on - strand, within RALBFv3_RS05965 at 1327.913 kb on + strand, within RALBFv3_RS05965 at 1327.913 kb on + strand, within RALBFv3_RS05965 at 1327.916 kb on + strand, within RALBFv3_RS05965 at 1327.917 kb on - strand, within RALBFv3_RS05965 at 1328.028 kb on - strand, within RALBFv3_RS05965 at 1328.112 kb on - strand, within RALBFv3_RS05965 at 1328.237 kb on + strand, within RALBFv3_RS05965 at 1328.237 kb on + strand, within RALBFv3_RS05965 at 1328.237 kb on + strand, within RALBFv3_RS05965 at 1328.238 kb on - strand, within RALBFv3_RS05965 at 1328.238 kb on - strand, within RALBFv3_RS05965 at 1328.753 kb on + strand, within RALBFv3_RS05970 at 1329.251 kb on + strand, within RALBFv3_RS05970 at 1329.473 kb on + strand, within RALBFv3_RS05970 at 1329.473 kb on + strand, within RALBFv3_RS05970 at 1329.473 kb on + strand, within RALBFv3_RS05970 at 1329.542 kb on + strand, within RALBFv3_RS05970 at 1329.632 kb on + strand at 1329.751 kb on + strand at 1329.752 kb on - strand at 1329.768 kb on + strand at 1329.769 kb on - strand at 1329.769 kb on - strand at 1329.769 kb on - strand at 1329.847 kb on - strand at 1330.143 kb on - strand, within RALBFv3_RS05975 at 1330.143 kb on - strand, within RALBFv3_RS05975 at 1330.428 kb on - strand, within RALBFv3_RS05975 at 1330.443 kb on + strand, within RALBFv3_RS05975 at 1330.444 kb on - strand, within RALBFv3_RS05975 at 1330.544 kb on + strand at 1330.545 kb on - strand at 1330.545 kb on - strand at 1330.545 kb on - strand at 1330.571 kb on - strand at 1330.572 kb on + strand at 1330.573 kb on - strand at 1330.575 kb on - strand at 1330.671 kb on - strand, within RALBFv3_RS05980
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,327,506 - RALBFv3_RS05960 0.64 -0.8 1,327,648 - RALBFv3_RS05960 0.89 -0.5 1,327,794 - RALBFv3_RS05965 0.13 +0.2 1,327,913 + RALBFv3_RS05965 0.32 -1.1 1,327,913 + RALBFv3_RS05965 0.32 -1.0 1,327,916 + RALBFv3_RS05965 0.32 -1.3 1,327,917 - RALBFv3_RS05965 0.33 -1.3 1,328,028 - RALBFv3_RS05965 0.50 -0.7 1,328,112 - RALBFv3_RS05965 0.63 -0.1 1,328,237 + RALBFv3_RS05965 0.83 -0.6 1,328,237 + RALBFv3_RS05965 0.83 -1.4 1,328,237 + RALBFv3_RS05965 0.83 -1.4 1,328,238 - RALBFv3_RS05965 0.83 -2.2 1,328,238 - RALBFv3_RS05965 0.83 -1.1 1,328,753 + RALBFv3_RS05970 0.29 -1.0 1,329,251 + RALBFv3_RS05970 0.65 -1.3 1,329,473 + RALBFv3_RS05970 0.81 -1.4 1,329,473 + RALBFv3_RS05970 0.81 -1.3 1,329,473 + RALBFv3_RS05970 0.81 -0.9 1,329,542 + RALBFv3_RS05970 0.86 -1.3 1,329,632 + -1.3 1,329,751 + -0.7 1,329,752 - -0.1 1,329,768 + +0.2 1,329,769 - +0.0 1,329,769 - +0.3 1,329,769 - +0.0 1,329,847 - +0.2 1,330,143 - RALBFv3_RS05975 0.43 +0.1 1,330,143 - RALBFv3_RS05975 0.43 +0.3 1,330,428 - RALBFv3_RS05975 0.85 -0.4 1,330,443 + RALBFv3_RS05975 0.87 -0.3 1,330,444 - RALBFv3_RS05975 0.87 +0.4 1,330,544 + -0.9 1,330,545 - -0.1 1,330,545 - -0.3 1,330,545 - +0.5 1,330,571 - +0.5 1,330,572 + +0.1 1,330,573 - +0.4 1,330,575 - +0.9 1,330,671 - RALBFv3_RS05980 0.13 -0.5
Or see this region's nucleotide sequence