Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05965

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05955 and RALBFv3_RS05960 are separated by 147 nucleotidesRALBFv3_RS05960 and RALBFv3_RS05965 overlap by 4 nucleotidesRALBFv3_RS05965 and RALBFv3_RS05970 are separated by 6 nucleotides RALBFv3_RS05955: RALBFv3_RS05955 - RNB domain-containing ribonuclease, at 1,324,898 to 1,326,979 _RS05955 RALBFv3_RS05960: RALBFv3_RS05960 - chorismate lyase, at 1,327,127 to 1,327,714 _RS05960 RALBFv3_RS05965: RALBFv3_RS05965 - esterase, at 1,327,711 to 1,328,343 _RS05965 RALBFv3_RS05970: RALBFv3_RS05970 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 1,328,350 to 1,329,735 _RS05970 Position (kb) 1327 1328 1329Strain fitness (log2 ratio) -2 -1 0 1at 1327.087 kb on - strandat 1327.125 kb on + strandat 1327.506 kb on - strand, within RALBFv3_RS05960at 1327.648 kb on - strand, within RALBFv3_RS05960at 1327.794 kb on - strand, within RALBFv3_RS05965at 1327.913 kb on + strand, within RALBFv3_RS05965at 1327.913 kb on + strand, within RALBFv3_RS05965at 1327.916 kb on + strand, within RALBFv3_RS05965at 1327.917 kb on - strand, within RALBFv3_RS05965at 1328.028 kb on - strand, within RALBFv3_RS05965at 1328.112 kb on - strand, within RALBFv3_RS05965at 1328.237 kb on + strand, within RALBFv3_RS05965at 1328.237 kb on + strand, within RALBFv3_RS05965at 1328.237 kb on + strand, within RALBFv3_RS05965at 1328.238 kb on - strand, within RALBFv3_RS05965at 1328.238 kb on - strand, within RALBFv3_RS05965at 1328.753 kb on + strand, within RALBFv3_RS05970at 1329.251 kb on + strand, within RALBFv3_RS05970

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,327,087 - -0.7
1,327,125 + -0.3
1,327,506 - RALBFv3_RS05960 0.64 -0.8
1,327,648 - RALBFv3_RS05960 0.89 -0.5
1,327,794 - RALBFv3_RS05965 0.13 +0.2
1,327,913 + RALBFv3_RS05965 0.32 -1.1
1,327,913 + RALBFv3_RS05965 0.32 -1.0
1,327,916 + RALBFv3_RS05965 0.32 -1.3
1,327,917 - RALBFv3_RS05965 0.33 -1.3
1,328,028 - RALBFv3_RS05965 0.50 -0.7
1,328,112 - RALBFv3_RS05965 0.63 -0.1
1,328,237 + RALBFv3_RS05965 0.83 -0.6
1,328,237 + RALBFv3_RS05965 0.83 -1.4
1,328,237 + RALBFv3_RS05965 0.83 -1.4
1,328,238 - RALBFv3_RS05965 0.83 -2.2
1,328,238 - RALBFv3_RS05965 0.83 -1.1
1,328,753 + RALBFv3_RS05970 0.29 -1.0
1,329,251 + RALBFv3_RS05970 0.65 -1.3

Or see this region's nucleotide sequence