Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05945

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05935 and RALBFv3_RS05940 are separated by 145 nucleotidesRALBFv3_RS05940 and RALBFv3_RS05945 are separated by 19 nucleotidesRALBFv3_RS05945 and RALBFv3_RS05950 overlap by 4 nucleotidesRALBFv3_RS05950 and RALBFv3_RS05955 are separated by 63 nucleotides RALBFv3_RS05935: RALBFv3_RS05935 - HARPIN, at 1,320,982 to 1,322,136 _RS05935 RALBFv3_RS05940: RALBFv3_RS05940 - monofunctional biosynthetic peptidoglycan transglycosylase, at 1,322,282 to 1,322,986 _RS05940 RALBFv3_RS05945: RALBFv3_RS05945 - shikimate dehydrogenase, at 1,323,006 to 1,323,923 _RS05945 RALBFv3_RS05950: RALBFv3_RS05950 - energy transducer TonB, at 1,323,920 to 1,324,834 _RS05950 RALBFv3_RS05955: RALBFv3_RS05955 - RNB domain-containing ribonuclease, at 1,324,898 to 1,326,979 _RS05955 Position (kb) 1323 1324Strain fitness (log2 ratio) -2 -1 0 1 2at 1322.032 kb on + strandat 1322.032 kb on + strandat 1322.032 kb on + strandat 1322.032 kb on + strandat 1322.032 kb on + strandat 1322.033 kb on - strandat 1322.033 kb on - strandat 1322.033 kb on - strandat 1322.055 kb on + strandat 1322.123 kb on - strandat 1322.123 kb on - strandat 1322.192 kb on - strandat 1322.347 kb on + strandat 1322.457 kb on + strand, within RALBFv3_RS05940at 1322.457 kb on + strand, within RALBFv3_RS05940at 1322.458 kb on - strand, within RALBFv3_RS05940at 1322.595 kb on + strand, within RALBFv3_RS05940at 1322.596 kb on - strand, within RALBFv3_RS05940at 1322.967 kb on + strandat 1322.967 kb on + strandat 1323.211 kb on + strand, within RALBFv3_RS05945at 1323.211 kb on + strand, within RALBFv3_RS05945at 1323.212 kb on - strand, within RALBFv3_RS05945at 1323.223 kb on + strand, within RALBFv3_RS05945at 1323.247 kb on - strand, within RALBFv3_RS05945at 1323.670 kb on + strand, within RALBFv3_RS05945at 1323.766 kb on + strand, within RALBFv3_RS05945at 1323.766 kb on + strand, within RALBFv3_RS05945at 1323.766 kb on + strand, within RALBFv3_RS05945at 1323.779 kb on - strand, within RALBFv3_RS05945at 1324.117 kb on - strand, within RALBFv3_RS05950at 1324.132 kb on - strand, within RALBFv3_RS05950at 1324.254 kb on + strand, within RALBFv3_RS05950at 1324.254 kb on + strand, within RALBFv3_RS05950at 1324.254 kb on + strand, within RALBFv3_RS05950at 1324.267 kb on - strand, within RALBFv3_RS05950at 1324.323 kb on + strand, within RALBFv3_RS05950at 1324.596 kb on + strand, within RALBFv3_RS05950at 1324.753 kb on + strandat 1324.754 kb on - strandat 1324.786 kb on + strandat 1324.895 kb on + strandat 1324.895 kb on + strandat 1324.896 kb on - strandat 1324.896 kb on - strandat 1324.896 kb on - strandat 1324.896 kb on - strandat 1324.896 kb on - strandat 1324.899 kb on + strandat 1324.899 kb on + strandat 1324.899 kb on + strandat 1324.900 kb on - strandat 1324.900 kb on - strandat 1324.900 kb on - strandat 1324.904 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,322,032 + -0.8
1,322,032 + +1.1
1,322,032 + +1.1
1,322,032 + +0.5
1,322,032 + +1.1
1,322,033 - -0.4
1,322,033 - +0.7
1,322,033 - +0.6
1,322,055 + +0.2
1,322,123 - +0.3
1,322,123 - +0.1
1,322,192 - +0.1
1,322,347 + +1.1
1,322,457 + RALBFv3_RS05940 0.25 +0.2
1,322,457 + RALBFv3_RS05940 0.25 +0.2
1,322,458 - RALBFv3_RS05940 0.25 -0.2
1,322,595 + RALBFv3_RS05940 0.44 -0.7
1,322,596 - RALBFv3_RS05940 0.45 -0.2
1,322,967 + -1.2
1,322,967 + -0.2
1,323,211 + RALBFv3_RS05945 0.22 -0.1
1,323,211 + RALBFv3_RS05945 0.22 -0.9
1,323,212 - RALBFv3_RS05945 0.22 -1.3
1,323,223 + RALBFv3_RS05945 0.24 +1.1
1,323,247 - RALBFv3_RS05945 0.26 -1.5
1,323,670 + RALBFv3_RS05945 0.72 -0.7
1,323,766 + RALBFv3_RS05945 0.83 +1.1
1,323,766 + RALBFv3_RS05945 0.83 +1.1
1,323,766 + RALBFv3_RS05945 0.83 -0.4
1,323,779 - RALBFv3_RS05945 0.84 +1.1
1,324,117 - RALBFv3_RS05950 0.22 -0.0
1,324,132 - RALBFv3_RS05950 0.23 -0.2
1,324,254 + RALBFv3_RS05950 0.37 +0.4
1,324,254 + RALBFv3_RS05950 0.37 +1.8
1,324,254 + RALBFv3_RS05950 0.37 +1.1
1,324,267 - RALBFv3_RS05950 0.38 +0.1
1,324,323 + RALBFv3_RS05950 0.44 -0.4
1,324,596 + RALBFv3_RS05950 0.74 -0.5
1,324,753 + -0.1
1,324,754 - -0.5
1,324,786 + -1.0
1,324,895 + +1.1
1,324,895 + +1.6
1,324,896 - -0.6
1,324,896 - -0.2
1,324,896 - +0.1
1,324,896 - -0.4
1,324,896 - -1.2
1,324,899 + +0.6
1,324,899 + -1.8
1,324,899 + +0.6
1,324,900 - +0.1
1,324,900 - +0.4
1,324,900 - +0.6
1,324,904 - -0.3

Or see this region's nucleotide sequence