Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05690

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05685 and RALBFv3_RS05690 are separated by 105 nucleotidesRALBFv3_RS05690 and RALBFv3_RS05695 are separated by 121 nucleotides RALBFv3_RS05685: RALBFv3_RS05685 - tRNA guanosine(34) transglycosylase Tgt, at 1,265,025 to 1,266,176 _RS05685 RALBFv3_RS05690: RALBFv3_RS05690 - preprotein translocase subunit YajC, at 1,266,282 to 1,266,617 _RS05690 RALBFv3_RS05695: RALBFv3_RS05695 - protein translocase subunit SecD, at 1,266,739 to 1,268,622 _RS05695 Position (kb) 1266 1267Strain fitness (log2 ratio) -1 0 1at 1265.787 kb on + strand, within RALBFv3_RS05685at 1265.788 kb on - strand, within RALBFv3_RS05685at 1265.977 kb on - strand, within RALBFv3_RS05685at 1266.072 kb on + strandat 1266.072 kb on + strandat 1266.075 kb on + strandat 1266.076 kb on - strandat 1266.227 kb on + strandat 1266.229 kb on - strandat 1266.522 kb on + strand, within RALBFv3_RS05690at 1266.522 kb on + strand, within RALBFv3_RS05690

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,265,787 + RALBFv3_RS05685 0.66 +1.0
1,265,788 - RALBFv3_RS05685 0.66 -1.0
1,265,977 - RALBFv3_RS05685 0.83 +1.0
1,266,072 + +0.2
1,266,072 + -0.9
1,266,075 + +0.6
1,266,076 - +0.5
1,266,227 + -0.3
1,266,229 - +1.1
1,266,522 + RALBFv3_RS05690 0.71 +1.1
1,266,522 + RALBFv3_RS05690 0.71 -1.1

Or see this region's nucleotide sequence