Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05675

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05665 and RALBFv3_RS05670 are separated by 11 nucleotidesRALBFv3_RS05670 and RALBFv3_RS05675 are separated by 32 nucleotidesRALBFv3_RS05675 and RALBFv3_RS05680 are separated by 76 nucleotides RALBFv3_RS05665: RALBFv3_RS05665 - hypothetical protein, at 1,260,986 to 1,261,426 _RS05665 RALBFv3_RS05670: RALBFv3_RS05670 - hypothetical protein, at 1,261,438 to 1,261,641 _RS05670 RALBFv3_RS05675: RALBFv3_RS05675 - DNA helicase RecG, at 1,261,674 to 1,263,866 _RS05675 RALBFv3_RS05680: RALBFv3_RS05680 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 1,263,943 to 1,265,031 _RS05680 Position (kb) 1261 1262 1263 1264Strain fitness (log2 ratio) -2 -1 0 1 2at 1260.711 kb on + strandat 1260.711 kb on + strandat 1260.712 kb on - strandat 1260.741 kb on + strandat 1260.782 kb on - strandat 1260.807 kb on + strandat 1260.807 kb on + strandat 1260.950 kb on + strandat 1261.144 kb on - strand, within RALBFv3_RS05665at 1261.205 kb on + strand, within RALBFv3_RS05665at 1261.205 kb on + strand, within RALBFv3_RS05665at 1261.205 kb on + strand, within RALBFv3_RS05665at 1261.205 kb on + strand, within RALBFv3_RS05665at 1261.205 kb on + strand, within RALBFv3_RS05665at 1261.206 kb on - strand, within RALBFv3_RS05665at 1261.206 kb on - strand, within RALBFv3_RS05665at 1261.206 kb on - strand, within RALBFv3_RS05665at 1261.206 kb on - strand, within RALBFv3_RS05665at 1261.206 kb on - strand, within RALBFv3_RS05665at 1261.206 kb on - strand, within RALBFv3_RS05665at 1261.208 kb on + strand, within RALBFv3_RS05665at 1261.208 kb on + strand, within RALBFv3_RS05665at 1261.208 kb on + strand, within RALBFv3_RS05665at 1261.208 kb on + strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.209 kb on - strand, within RALBFv3_RS05665at 1261.687 kb on + strandat 1261.687 kb on + strandat 1261.688 kb on - strandat 1261.688 kb on - strandat 1261.963 kb on + strand, within RALBFv3_RS05675at 1261.963 kb on + strand, within RALBFv3_RS05675at 1261.964 kb on - strand, within RALBFv3_RS05675at 1261.964 kb on - strand, within RALBFv3_RS05675at 1262.296 kb on + strand, within RALBFv3_RS05675at 1262.297 kb on - strand, within RALBFv3_RS05675at 1262.297 kb on - strand, within RALBFv3_RS05675at 1262.437 kb on + strand, within RALBFv3_RS05675at 1262.437 kb on + strand, within RALBFv3_RS05675at 1262.437 kb on + strand, within RALBFv3_RS05675at 1262.437 kb on + strand, within RALBFv3_RS05675at 1262.437 kb on + strand, within RALBFv3_RS05675at 1262.438 kb on - strand, within RALBFv3_RS05675at 1262.438 kb on - strand, within RALBFv3_RS05675at 1262.438 kb on - strand, within RALBFv3_RS05675at 1263.307 kb on + strand, within RALBFv3_RS05675at 1263.307 kb on + strand, within RALBFv3_RS05675at 1263.337 kb on + strand, within RALBFv3_RS05675at 1263.337 kb on + strand, within RALBFv3_RS05675at 1263.487 kb on + strand, within RALBFv3_RS05675at 1263.488 kb on - strand, within RALBFv3_RS05675at 1263.667 kb on + strandat 1263.667 kb on + strandat 1263.875 kb on + strandat 1263.875 kb on + strandat 1263.876 kb on - strandat 1263.876 kb on - strandat 1263.876 kb on - strandat 1263.909 kb on + strandat 1263.909 kb on + strandat 1264.114 kb on + strand, within RALBFv3_RS05680at 1264.387 kb on + strand, within RALBFv3_RS05680at 1264.387 kb on + strand, within RALBFv3_RS05680at 1264.411 kb on + strand, within RALBFv3_RS05680at 1264.413 kb on + strand, within RALBFv3_RS05680at 1264.414 kb on - strand, within RALBFv3_RS05680at 1264.414 kb on - strand, within RALBFv3_RS05680at 1264.414 kb on - strand, within RALBFv3_RS05680at 1264.463 kb on - strand, within RALBFv3_RS05680

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,260,711 + -0.1
1,260,711 + -0.4
1,260,712 - -0.5
1,260,741 + +0.2
1,260,782 - +0.2
1,260,807 + +0.2
1,260,807 + -0.2
1,260,950 + +0.2
1,261,144 - RALBFv3_RS05665 0.36 +0.2
1,261,205 + RALBFv3_RS05665 0.50 -0.2
1,261,205 + RALBFv3_RS05665 0.50 +0.4
1,261,205 + RALBFv3_RS05665 0.50 +0.2
1,261,205 + RALBFv3_RS05665 0.50 -0.3
1,261,205 + RALBFv3_RS05665 0.50 -0.1
1,261,206 - RALBFv3_RS05665 0.50 +0.2
1,261,206 - RALBFv3_RS05665 0.50 +0.2
1,261,206 - RALBFv3_RS05665 0.50 -0.1
1,261,206 - RALBFv3_RS05665 0.50 +0.4
1,261,206 - RALBFv3_RS05665 0.50 +1.3
1,261,206 - RALBFv3_RS05665 0.50 -0.1
1,261,208 + RALBFv3_RS05665 0.50 +1.3
1,261,208 + RALBFv3_RS05665 0.50 +1.0
1,261,208 + RALBFv3_RS05665 0.50 +0.2
1,261,208 + RALBFv3_RS05665 0.50 -0.8
1,261,209 - RALBFv3_RS05665 0.51 +0.3
1,261,209 - RALBFv3_RS05665 0.51 +0.5
1,261,209 - RALBFv3_RS05665 0.51 -0.0
1,261,209 - RALBFv3_RS05665 0.51 +0.3
1,261,209 - RALBFv3_RS05665 0.51 -0.4
1,261,209 - RALBFv3_RS05665 0.51 +0.2
1,261,209 - RALBFv3_RS05665 0.51 -0.2
1,261,209 - RALBFv3_RS05665 0.51 -1.0
1,261,209 - RALBFv3_RS05665 0.51 -0.3
1,261,209 - RALBFv3_RS05665 0.51 +0.0
1,261,209 - RALBFv3_RS05665 0.51 +0.2
1,261,687 + -0.5
1,261,687 + +1.5
1,261,688 - -0.2
1,261,688 - +0.1
1,261,963 + RALBFv3_RS05675 0.13 -1.0
1,261,963 + RALBFv3_RS05675 0.13 -0.8
1,261,964 - RALBFv3_RS05675 0.13 -2.1
1,261,964 - RALBFv3_RS05675 0.13 -1.0
1,262,296 + RALBFv3_RS05675 0.28 -0.7
1,262,297 - RALBFv3_RS05675 0.28 -0.3
1,262,297 - RALBFv3_RS05675 0.28 -2.5
1,262,437 + RALBFv3_RS05675 0.35 -1.3
1,262,437 + RALBFv3_RS05675 0.35 -0.7
1,262,437 + RALBFv3_RS05675 0.35 +1.0
1,262,437 + RALBFv3_RS05675 0.35 -1.1
1,262,437 + RALBFv3_RS05675 0.35 -0.7
1,262,438 - RALBFv3_RS05675 0.35 -0.9
1,262,438 - RALBFv3_RS05675 0.35 -1.4
1,262,438 - RALBFv3_RS05675 0.35 -1.7
1,263,307 + RALBFv3_RS05675 0.74 -0.8
1,263,307 + RALBFv3_RS05675 0.74 +0.3
1,263,337 + RALBFv3_RS05675 0.76 +0.0
1,263,337 + RALBFv3_RS05675 0.76 -0.1
1,263,487 + RALBFv3_RS05675 0.83 -1.3
1,263,488 - RALBFv3_RS05675 0.83 -1.0
1,263,667 + -1.4
1,263,667 + -1.6
1,263,875 + -1.5
1,263,875 + -1.6
1,263,876 - +0.0
1,263,876 - -0.9
1,263,876 - +0.5
1,263,909 + -0.4
1,263,909 + -0.4
1,264,114 + RALBFv3_RS05680 0.16 +1.5
1,264,387 + RALBFv3_RS05680 0.41 +0.0
1,264,387 + RALBFv3_RS05680 0.41 +1.0
1,264,411 + RALBFv3_RS05680 0.43 +1.0
1,264,413 + RALBFv3_RS05680 0.43 +1.0
1,264,414 - RALBFv3_RS05680 0.43 +0.0
1,264,414 - RALBFv3_RS05680 0.43 +2.0
1,264,414 - RALBFv3_RS05680 0.43 -2.1
1,264,463 - RALBFv3_RS05680 0.48 -0.8

Or see this region's nucleotide sequence