Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05415 and RALBFv3_RS05420 are separated by 81 nucleotides RALBFv3_RS05420 and RALBFv3_RS05425 are separated by 192 nucleotides
RALBFv3_RS05415: RALBFv3_RS05415 - DNA repair protein RecN, at 1,193,409 to 1,195,118
_RS05415
RALBFv3_RS05420: RALBFv3_RS05420 - hypothetical protein, at 1,195,200 to 1,195,646
_RS05420
RALBFv3_RS05425: RALBFv3_RS05425 - MprA protease, GlyGly-CTERM protein-sorting domain-containing form, at 1,195,839 to 1,197,458
_RS05425
Position (kb)
1195
1196 Strain fitness (log2 ratio)
-2
-1
0
1 at 1194.261 kb on + strand, within RALBFv3_RS05415 at 1194.262 kb on - strand, within RALBFv3_RS05415 at 1194.346 kb on - strand, within RALBFv3_RS05415 at 1194.347 kb on - strand, within RALBFv3_RS05415 at 1195.124 kb on + strand at 1195.126 kb on + strand at 1195.127 kb on - strand at 1195.192 kb on - strand at 1195.252 kb on + strand, within RALBFv3_RS05420 at 1195.485 kb on + strand, within RALBFv3_RS05420 at 1195.485 kb on + strand, within RALBFv3_RS05420 at 1195.486 kb on - strand, within RALBFv3_RS05420 at 1195.486 kb on - strand, within RALBFv3_RS05420 at 1195.531 kb on + strand, within RALBFv3_RS05420 at 1195.532 kb on - strand, within RALBFv3_RS05420 at 1195.532 kb on - strand, within RALBFv3_RS05420 at 1195.532 kb on - strand, within RALBFv3_RS05420 at 1195.625 kb on + strand at 1195.805 kb on - strand at 1195.805 kb on - strand at 1195.957 kb on - strand at 1196.187 kb on + strand, within RALBFv3_RS05425 at 1196.187 kb on + strand, within RALBFv3_RS05425 at 1196.187 kb on + strand, within RALBFv3_RS05425 at 1196.187 kb on + strand, within RALBFv3_RS05425 at 1196.188 kb on - strand, within RALBFv3_RS05425 at 1196.188 kb on - strand, within RALBFv3_RS05425 at 1196.188 kb on - strand, within RALBFv3_RS05425 at 1196.188 kb on - strand, within RALBFv3_RS05425 at 1196.188 kb on - strand, within RALBFv3_RS05425 at 1196.188 kb on - strand, within RALBFv3_RS05425 at 1196.363 kb on + strand, within RALBFv3_RS05425 at 1196.363 kb on + strand, within RALBFv3_RS05425
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,194,261 + RALBFv3_RS05415 0.50 +0.3 1,194,262 - RALBFv3_RS05415 0.50 +1.2 1,194,346 - RALBFv3_RS05415 0.55 -0.3 1,194,347 - RALBFv3_RS05415 0.55 -1.2 1,195,124 + +0.4 1,195,126 + -0.5 1,195,127 - -0.6 1,195,192 - -1.3 1,195,252 + RALBFv3_RS05420 0.12 +0.0 1,195,485 + RALBFv3_RS05420 0.64 +0.2 1,195,485 + RALBFv3_RS05420 0.64 -0.2 1,195,486 - RALBFv3_RS05420 0.64 -0.1 1,195,486 - RALBFv3_RS05420 0.64 -0.5 1,195,531 + RALBFv3_RS05420 0.74 -0.7 1,195,532 - RALBFv3_RS05420 0.74 -0.4 1,195,532 - RALBFv3_RS05420 0.74 +0.0 1,195,532 - RALBFv3_RS05420 0.74 +0.5 1,195,625 + +0.3 1,195,805 - +0.0 1,195,805 - -0.2 1,195,957 - +1.0 1,196,187 + RALBFv3_RS05425 0.21 -0.4 1,196,187 + RALBFv3_RS05425 0.21 -0.2 1,196,187 + RALBFv3_RS05425 0.21 -0.5 1,196,187 + RALBFv3_RS05425 0.21 +1.0 1,196,188 - RALBFv3_RS05425 0.22 -1.8 1,196,188 - RALBFv3_RS05425 0.22 -0.1 1,196,188 - RALBFv3_RS05425 0.22 +0.2 1,196,188 - RALBFv3_RS05425 0.22 +0.1 1,196,188 - RALBFv3_RS05425 0.22 -0.5 1,196,188 - RALBFv3_RS05425 0.22 -0.4 1,196,363 + RALBFv3_RS05425 0.32 -0.4 1,196,363 + RALBFv3_RS05425 0.32 +0.0
Or see this region's nucleotide sequence