Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05390

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05380 and RALBFv3_RS05385 overlap by 8 nucleotidesRALBFv3_RS05385 and RALBFv3_RS05390 overlap by 4 nucleotidesRALBFv3_RS05390 and RALBFv3_RS05395 are separated by 18 nucleotides RALBFv3_RS05380: RALBFv3_RS05380 - imidazolonepropionase, at 1,184,836 to 1,186,062 _RS05380 RALBFv3_RS05385: RALBFv3_RS05385 - formimidoylglutamase, at 1,186,055 to 1,187,038 _RS05385 RALBFv3_RS05390: RALBFv3_RS05390 - histidine ammonia-lyase, at 1,187,035 to 1,188,591 _RS05390 RALBFv3_RS05395: RALBFv3_RS05395 - urocanate hydratase, at 1,188,610 to 1,190,415 _RS05395 Position (kb) 1187 1188 1189Strain fitness (log2 ratio) -1 0 1at 1186.308 kb on + strand, within RALBFv3_RS05385at 1186.308 kb on + strand, within RALBFv3_RS05385at 1186.470 kb on + strand, within RALBFv3_RS05385at 1187.148 kb on - strandat 1187.150 kb on + strandat 1187.151 kb on - strandat 1188.179 kb on - strand, within RALBFv3_RS05390at 1189.160 kb on + strand, within RALBFv3_RS05395at 1189.160 kb on + strand, within RALBFv3_RS05395at 1189.160 kb on + strand, within RALBFv3_RS05395at 1189.160 kb on + strand, within RALBFv3_RS05395at 1189.161 kb on - strand, within RALBFv3_RS05395at 1189.161 kb on - strand, within RALBFv3_RS05395at 1189.161 kb on - strand, within RALBFv3_RS05395at 1189.235 kb on + strand, within RALBFv3_RS05395at 1189.235 kb on + strand, within RALBFv3_RS05395at 1189.235 kb on + strand, within RALBFv3_RS05395at 1189.236 kb on - strand, within RALBFv3_RS05395at 1189.314 kb on - strand, within RALBFv3_RS05395

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,186,308 + RALBFv3_RS05385 0.26 +0.3
1,186,308 + RALBFv3_RS05385 0.26 +0.1
1,186,470 + RALBFv3_RS05385 0.42 -0.4
1,187,148 - -1.1
1,187,150 + +0.5
1,187,151 - -0.5
1,188,179 - RALBFv3_RS05390 0.73 -0.8
1,189,160 + RALBFv3_RS05395 0.30 +0.4
1,189,160 + RALBFv3_RS05395 0.30 -0.2
1,189,160 + RALBFv3_RS05395 0.30 -0.1
1,189,160 + RALBFv3_RS05395 0.30 +0.0
1,189,161 - RALBFv3_RS05395 0.31 +0.8
1,189,161 - RALBFv3_RS05395 0.31 +0.4
1,189,161 - RALBFv3_RS05395 0.31 +0.3
1,189,235 + RALBFv3_RS05395 0.35 +0.3
1,189,235 + RALBFv3_RS05395 0.35 +0.4
1,189,235 + RALBFv3_RS05395 0.35 -0.3
1,189,236 - RALBFv3_RS05395 0.35 -0.3
1,189,314 - RALBFv3_RS05395 0.39 -0.3

Or see this region's nucleotide sequence