Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05200 and RALBFv3_RS05205 overlap by 1 nucleotides RALBFv3_RS05205 and RALBFv3_RS05210 are separated by 3 nucleotides RALBFv3_RS05210 and RALBFv3_RS05215 are separated by 232 nucleotides
RALBFv3_RS05200: RALBFv3_RS05200 - murein biosynthesis integral membrane protein MurJ, at 1,151,783 to 1,153,375
_RS05200
RALBFv3_RS05205: RALBFv3_RS05205 - transglutaminase, at 1,153,375 to 1,154,220
_RS05205
RALBFv3_RS05210: RALBFv3_RS05210 - membrane protein, at 1,154,224 to 1,154,880
_RS05210
RALBFv3_RS05215: RALBFv3_RS05215 - TetR/AcrR family transcriptional regulator, at 1,155,113 to 1,155,742
_RS05215
Position (kb)
1154
1155 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1153.630 kb on + strand, within RALBFv3_RS05205 at 1153.633 kb on + strand, within RALBFv3_RS05205 at 1153.633 kb on + strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.634 kb on - strand, within RALBFv3_RS05205 at 1153.651 kb on + strand, within RALBFv3_RS05205 at 1153.805 kb on + strand, within RALBFv3_RS05205 at 1153.806 kb on - strand, within RALBFv3_RS05205 at 1153.868 kb on - strand, within RALBFv3_RS05205 at 1153.982 kb on - strand, within RALBFv3_RS05205 at 1153.982 kb on - strand, within RALBFv3_RS05205 at 1153.982 kb on - strand, within RALBFv3_RS05205 at 1154.084 kb on - strand, within RALBFv3_RS05205 at 1154.131 kb on + strand, within RALBFv3_RS05205 at 1154.131 kb on + strand, within RALBFv3_RS05205 at 1154.132 kb on - strand, within RALBFv3_RS05205 at 1154.132 kb on - strand, within RALBFv3_RS05205 at 1154.132 kb on - strand, within RALBFv3_RS05205 at 1154.225 kb on + strand at 1154.225 kb on + strand at 1154.226 kb on - strand at 1154.529 kb on - strand, within RALBFv3_RS05210 at 1154.575 kb on - strand, within RALBFv3_RS05210 at 1154.619 kb on - strand, within RALBFv3_RS05210 at 1154.719 kb on + strand, within RALBFv3_RS05210 at 1154.720 kb on - strand, within RALBFv3_RS05210 at 1154.924 kb on + strand at 1154.924 kb on + strand at 1154.925 kb on - strand at 1155.108 kb on + strand at 1155.108 kb on + strand at 1155.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,153,630 + RALBFv3_RS05205 0.30 -0.1 1,153,633 + RALBFv3_RS05205 0.30 -0.2 1,153,633 + RALBFv3_RS05205 0.30 -2.4 1,153,634 - RALBFv3_RS05205 0.31 +1.3 1,153,634 - RALBFv3_RS05205 0.31 -0.2 1,153,634 - RALBFv3_RS05205 0.31 +0.3 1,153,634 - RALBFv3_RS05205 0.31 +0.0 1,153,634 - RALBFv3_RS05205 0.31 +1.0 1,153,634 - RALBFv3_RS05205 0.31 +1.0 1,153,634 - RALBFv3_RS05205 0.31 -0.7 1,153,651 + RALBFv3_RS05205 0.33 -0.1 1,153,805 + RALBFv3_RS05205 0.51 +0.0 1,153,806 - RALBFv3_RS05205 0.51 -1.0 1,153,868 - RALBFv3_RS05205 0.58 +0.1 1,153,982 - RALBFv3_RS05205 0.72 +0.3 1,153,982 - RALBFv3_RS05205 0.72 -0.1 1,153,982 - RALBFv3_RS05205 0.72 +0.6 1,154,084 - RALBFv3_RS05205 0.84 -0.3 1,154,131 + RALBFv3_RS05205 0.89 -0.3 1,154,131 + RALBFv3_RS05205 0.89 -1.3 1,154,132 - RALBFv3_RS05205 0.89 -0.1 1,154,132 - RALBFv3_RS05205 0.89 -0.0 1,154,132 - RALBFv3_RS05205 0.89 -0.1 1,154,225 + -1.0 1,154,225 + -0.6 1,154,226 - -0.0 1,154,529 - RALBFv3_RS05210 0.46 +1.0 1,154,575 - RALBFv3_RS05210 0.53 -3.2 1,154,619 - RALBFv3_RS05210 0.60 -2.7 1,154,719 + RALBFv3_RS05210 0.75 -2.7 1,154,720 - RALBFv3_RS05210 0.75 +1.0 1,154,924 + -0.5 1,154,924 + +1.0 1,154,925 - +0.2 1,155,108 + -0.7 1,155,108 + -0.3 1,155,109 - -3.0
Or see this region's nucleotide sequence