Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05210

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05200 and RALBFv3_RS05205 overlap by 1 nucleotidesRALBFv3_RS05205 and RALBFv3_RS05210 are separated by 3 nucleotidesRALBFv3_RS05210 and RALBFv3_RS05215 are separated by 232 nucleotides RALBFv3_RS05200: RALBFv3_RS05200 - murein biosynthesis integral membrane protein MurJ, at 1,151,783 to 1,153,375 _RS05200 RALBFv3_RS05205: RALBFv3_RS05205 - transglutaminase, at 1,153,375 to 1,154,220 _RS05205 RALBFv3_RS05210: RALBFv3_RS05210 - membrane protein, at 1,154,224 to 1,154,880 _RS05210 RALBFv3_RS05215: RALBFv3_RS05215 - TetR/AcrR family transcriptional regulator, at 1,155,113 to 1,155,742 _RS05215 Position (kb) 1154 1155Strain fitness (log2 ratio) -3 -2 -1 0 1at 1153.630 kb on + strand, within RALBFv3_RS05205at 1153.633 kb on + strand, within RALBFv3_RS05205at 1153.633 kb on + strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.634 kb on - strand, within RALBFv3_RS05205at 1153.651 kb on + strand, within RALBFv3_RS05205at 1153.805 kb on + strand, within RALBFv3_RS05205at 1153.806 kb on - strand, within RALBFv3_RS05205at 1153.868 kb on - strand, within RALBFv3_RS05205at 1153.982 kb on - strand, within RALBFv3_RS05205at 1153.982 kb on - strand, within RALBFv3_RS05205at 1153.982 kb on - strand, within RALBFv3_RS05205at 1154.084 kb on - strand, within RALBFv3_RS05205at 1154.131 kb on + strand, within RALBFv3_RS05205at 1154.131 kb on + strand, within RALBFv3_RS05205at 1154.132 kb on - strand, within RALBFv3_RS05205at 1154.132 kb on - strand, within RALBFv3_RS05205at 1154.132 kb on - strand, within RALBFv3_RS05205at 1154.225 kb on + strandat 1154.225 kb on + strandat 1154.226 kb on - strandat 1154.529 kb on - strand, within RALBFv3_RS05210at 1154.575 kb on - strand, within RALBFv3_RS05210at 1154.619 kb on - strand, within RALBFv3_RS05210at 1154.719 kb on + strand, within RALBFv3_RS05210at 1154.720 kb on - strand, within RALBFv3_RS05210at 1154.924 kb on + strandat 1154.924 kb on + strandat 1154.925 kb on - strandat 1155.108 kb on + strandat 1155.108 kb on + strandat 1155.109 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,153,630 + RALBFv3_RS05205 0.30 -0.1
1,153,633 + RALBFv3_RS05205 0.30 -0.2
1,153,633 + RALBFv3_RS05205 0.30 -2.4
1,153,634 - RALBFv3_RS05205 0.31 +1.3
1,153,634 - RALBFv3_RS05205 0.31 -0.2
1,153,634 - RALBFv3_RS05205 0.31 +0.3
1,153,634 - RALBFv3_RS05205 0.31 +0.0
1,153,634 - RALBFv3_RS05205 0.31 +1.0
1,153,634 - RALBFv3_RS05205 0.31 +1.0
1,153,634 - RALBFv3_RS05205 0.31 -0.7
1,153,651 + RALBFv3_RS05205 0.33 -0.1
1,153,805 + RALBFv3_RS05205 0.51 +0.0
1,153,806 - RALBFv3_RS05205 0.51 -1.0
1,153,868 - RALBFv3_RS05205 0.58 +0.1
1,153,982 - RALBFv3_RS05205 0.72 +0.3
1,153,982 - RALBFv3_RS05205 0.72 -0.1
1,153,982 - RALBFv3_RS05205 0.72 +0.6
1,154,084 - RALBFv3_RS05205 0.84 -0.3
1,154,131 + RALBFv3_RS05205 0.89 -0.3
1,154,131 + RALBFv3_RS05205 0.89 -1.3
1,154,132 - RALBFv3_RS05205 0.89 -0.1
1,154,132 - RALBFv3_RS05205 0.89 -0.0
1,154,132 - RALBFv3_RS05205 0.89 -0.1
1,154,225 + -1.0
1,154,225 + -0.6
1,154,226 - -0.0
1,154,529 - RALBFv3_RS05210 0.46 +1.0
1,154,575 - RALBFv3_RS05210 0.53 -3.2
1,154,619 - RALBFv3_RS05210 0.60 -2.7
1,154,719 + RALBFv3_RS05210 0.75 -2.7
1,154,720 - RALBFv3_RS05210 0.75 +1.0
1,154,924 + -0.5
1,154,924 + +1.0
1,154,925 - +0.2
1,155,108 + -0.7
1,155,108 + -0.3
1,155,109 - -3.0

Or see this region's nucleotide sequence