Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05105 and RALBFv3_RS05110 are separated by 70 nucleotides RALBFv3_RS05110 and RALBFv3_RS05115 overlap by 7 nucleotides RALBFv3_RS05115 and RALBFv3_RS05120 are separated by 16 nucleotides
RALBFv3_RS05105: RALBFv3_RS05105 - bile acid:sodium symporter, at 1,134,563 to 1,135,582
_RS05105
RALBFv3_RS05110: RALBFv3_RS05110 - N-acetylmuramoyl-L-alanine amidase, at 1,135,653 to 1,137,179
_RS05110
RALBFv3_RS05115: RALBFv3_RS05115 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 1,137,173 to 1,137,742
_RS05115
RALBFv3_RS05120: RALBFv3_RS05120 - tRNA epoxyqueuosine(34) reductase QueG, at 1,137,759 to 1,138,919
_RS05120
Position (kb) 1135
1136
1137
1138 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1134.884 kb on + strand, within RALBFv3_RS05105 at 1134.885 kb on - strand, within RALBFv3_RS05105 at 1134.885 kb on - strand, within RALBFv3_RS05105 at 1135.152 kb on - strand, within RALBFv3_RS05105 at 1135.168 kb on + strand, within RALBFv3_RS05105 at 1135.248 kb on - strand, within RALBFv3_RS05105 at 1135.475 kb on + strand, within RALBFv3_RS05105 at 1135.475 kb on + strand, within RALBFv3_RS05105 at 1135.476 kb on - strand, within RALBFv3_RS05105 at 1135.476 kb on - strand, within RALBFv3_RS05105 at 1135.521 kb on - strand at 1135.696 kb on + strand at 1135.697 kb on - strand at 1135.697 kb on - strand at 1136.191 kb on + strand, within RALBFv3_RS05110 at 1136.191 kb on + strand, within RALBFv3_RS05110 at 1136.192 kb on - strand, within RALBFv3_RS05110 at 1136.192 kb on - strand, within RALBFv3_RS05110 at 1136.707 kb on + strand, within RALBFv3_RS05110 at 1136.995 kb on + strand, within RALBFv3_RS05110 at 1137.755 kb on - strand at 1137.970 kb on - strand, within RALBFv3_RS05120 at 1137.970 kb on - strand, within RALBFv3_RS05120 at 1137.970 kb on - strand, within RALBFv3_RS05120 at 1137.971 kb on + strand, within RALBFv3_RS05120 at 1137.971 kb on + strand, within RALBFv3_RS05120 at 1137.972 kb on - strand, within RALBFv3_RS05120 at 1137.972 kb on - strand, within RALBFv3_RS05120 at 1138.053 kb on + strand, within RALBFv3_RS05120 at 1138.053 kb on + strand, within RALBFv3_RS05120 at 1138.053 kb on + strand, within RALBFv3_RS05120 at 1138.053 kb on + strand, within RALBFv3_RS05120 at 1138.054 kb on - strand, within RALBFv3_RS05120 at 1138.054 kb on - strand, within RALBFv3_RS05120 at 1138.054 kb on - strand, within RALBFv3_RS05120 at 1138.054 kb on - strand, within RALBFv3_RS05120 at 1138.054 kb on - strand, within RALBFv3_RS05120 at 1138.170 kb on + strand, within RALBFv3_RS05120
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,134,884 + RALBFv3_RS05105 0.31 +0.1 1,134,885 - RALBFv3_RS05105 0.32 +0.0 1,134,885 - RALBFv3_RS05105 0.32 -0.2 1,135,152 - RALBFv3_RS05105 0.58 +0.3 1,135,168 + RALBFv3_RS05105 0.59 +1.0 1,135,248 - RALBFv3_RS05105 0.67 -0.1 1,135,475 + RALBFv3_RS05105 0.89 +1.0 1,135,475 + RALBFv3_RS05105 0.89 -0.4 1,135,476 - RALBFv3_RS05105 0.90 -1.8 1,135,476 - RALBFv3_RS05105 0.90 -0.1 1,135,521 - +0.5 1,135,696 + -0.6 1,135,697 - -0.7 1,135,697 - -1.0 1,136,191 + RALBFv3_RS05110 0.35 -0.7 1,136,191 + RALBFv3_RS05110 0.35 +0.0 1,136,192 - RALBFv3_RS05110 0.35 -1.3 1,136,192 - RALBFv3_RS05110 0.35 -1.8 1,136,707 + RALBFv3_RS05110 0.69 -1.0 1,136,995 + RALBFv3_RS05110 0.88 -0.8 1,137,755 - -1.5 1,137,970 - RALBFv3_RS05120 0.18 -0.4 1,137,970 - RALBFv3_RS05120 0.18 +0.1 1,137,970 - RALBFv3_RS05120 0.18 -0.7 1,137,971 + RALBFv3_RS05120 0.18 -0.5 1,137,971 + RALBFv3_RS05120 0.18 +1.0 1,137,972 - RALBFv3_RS05120 0.18 -0.6 1,137,972 - RALBFv3_RS05120 0.18 -0.5 1,138,053 + RALBFv3_RS05120 0.25 -0.4 1,138,053 + RALBFv3_RS05120 0.25 -0.6 1,138,053 + RALBFv3_RS05120 0.25 -0.4 1,138,053 + RALBFv3_RS05120 0.25 +0.2 1,138,054 - RALBFv3_RS05120 0.25 -0.1 1,138,054 - RALBFv3_RS05120 0.25 -0.6 1,138,054 - RALBFv3_RS05120 0.25 -0.7 1,138,054 - RALBFv3_RS05120 0.25 -2.9 1,138,054 - RALBFv3_RS05120 0.25 +1.0 1,138,170 + RALBFv3_RS05120 0.35 +1.4
Or see this region's nucleotide sequence