Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS04655 and RALBFv3_RS04660 are separated by 56 nucleotides RALBFv3_RS04660 and RALBFv3_RS04665 are separated by 262 nucleotides
RALBFv3_RS04655: RALBFv3_RS04655 - NAD-dependent dehydratase, at 1,041,031 to 1,042,056
_RS04655
RALBFv3_RS04660: RALBFv3_RS04660 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 1,042,113 to 1,043,162
_RS04660
RALBFv3_RS04665: RALBFv3_RS04665 - acetylornithine transaminase, at 1,043,425 to 1,044,612
_RS04665
Position (kb)
1042
1043
1044 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1041.231 kb on - strand, within RALBFv3_RS04655 at 1041.336 kb on + strand, within RALBFv3_RS04655 at 1041.337 kb on - strand, within RALBFv3_RS04655 at 1041.337 kb on - strand, within RALBFv3_RS04655 at 1041.420 kb on - strand, within RALBFv3_RS04655 at 1041.593 kb on + strand, within RALBFv3_RS04655 at 1041.594 kb on - strand, within RALBFv3_RS04655 at 1041.594 kb on - strand, within RALBFv3_RS04655 at 1041.655 kb on + strand, within RALBFv3_RS04655 at 1041.655 kb on + strand, within RALBFv3_RS04655 at 1042.109 kb on + strand at 1042.109 kb on + strand at 1042.109 kb on + strand at 1042.110 kb on - strand at 1042.112 kb on + strand at 1042.112 kb on + strand at 1042.112 kb on + strand at 1042.180 kb on + strand at 1042.521 kb on + strand, within RALBFv3_RS04660 at 1042.521 kb on + strand, within RALBFv3_RS04660 at 1042.522 kb on - strand, within RALBFv3_RS04660 at 1042.641 kb on + strand, within RALBFv3_RS04660 at 1042.641 kb on + strand, within RALBFv3_RS04660 at 1042.842 kb on + strand, within RALBFv3_RS04660 at 1042.873 kb on - strand, within RALBFv3_RS04660 at 1042.873 kb on - strand, within RALBFv3_RS04660 at 1043.221 kb on + strand at 1043.221 kb on + strand at 1043.266 kb on + strand at 1043.267 kb on - strand at 1043.313 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,041,231 - RALBFv3_RS04655 0.19 -0.0 1,041,336 + RALBFv3_RS04655 0.30 +0.0 1,041,337 - RALBFv3_RS04655 0.30 +0.3 1,041,337 - RALBFv3_RS04655 0.30 +0.1 1,041,420 - RALBFv3_RS04655 0.38 -0.2 1,041,593 + RALBFv3_RS04655 0.55 -0.8 1,041,594 - RALBFv3_RS04655 0.55 +1.1 1,041,594 - RALBFv3_RS04655 0.55 -0.2 1,041,655 + RALBFv3_RS04655 0.61 -0.1 1,041,655 + RALBFv3_RS04655 0.61 -0.3 1,042,109 + -0.5 1,042,109 + +2.1 1,042,109 + -1.3 1,042,110 - +1.1 1,042,112 + -0.9 1,042,112 + -0.5 1,042,112 + -0.2 1,042,180 + +0.1 1,042,521 + RALBFv3_RS04660 0.39 +1.1 1,042,521 + RALBFv3_RS04660 0.39 -0.5 1,042,522 - RALBFv3_RS04660 0.39 -1.3 1,042,641 + RALBFv3_RS04660 0.50 +0.1 1,042,641 + RALBFv3_RS04660 0.50 -1.8 1,042,842 + RALBFv3_RS04660 0.69 -1.8 1,042,873 - RALBFv3_RS04660 0.72 -0.9 1,042,873 - RALBFv3_RS04660 0.72 -0.5 1,043,221 + -0.5 1,043,221 + -0.0 1,043,266 + -0.1 1,043,267 - +0.1 1,043,313 + -0.3
Or see this region's nucleotide sequence