Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS04600 and RALBFv3_RS04605 are separated by 135 nucleotides RALBFv3_RS04605 and RALBFv3_RS04610 are separated by 161 nucleotides
RALBFv3_RS04600: RALBFv3_RS04600 - sulfite reductase, at 1,028,508 to 1,030,172
_RS04600
RALBFv3_RS04605: RALBFv3_RS04605 - sulfite exporter TauE/SafE family protein, at 1,030,308 to 1,031,081
_RS04605
RALBFv3_RS04610: RALBFv3_RS04610 - CysB family HTH-type transcriptional regulator, at 1,031,243 to 1,032,184
_RS04610
Position (kb)
1030
1031
1032 Strain fitness (log2 ratio)
-1
0
1
2 at 1029.462 kb on + strand, within RALBFv3_RS04600 at 1029.487 kb on + strand, within RALBFv3_RS04600 at 1029.488 kb on - strand, within RALBFv3_RS04600 at 1029.896 kb on - strand, within RALBFv3_RS04600 at 1030.018 kb on + strand at 1030.018 kb on + strand at 1030.036 kb on + strand at 1030.037 kb on - strand at 1030.169 kb on - strand at 1030.293 kb on + strand at 1030.425 kb on + strand, within RALBFv3_RS04605 at 1030.426 kb on - strand, within RALBFv3_RS04605 at 1030.468 kb on + strand, within RALBFv3_RS04605 at 1030.468 kb on + strand, within RALBFv3_RS04605 at 1030.469 kb on - strand, within RALBFv3_RS04605 at 1030.469 kb on - strand, within RALBFv3_RS04605 at 1030.469 kb on - strand, within RALBFv3_RS04605 at 1030.469 kb on - strand, within RALBFv3_RS04605 at 1030.511 kb on - strand, within RALBFv3_RS04605 at 1030.511 kb on - strand, within RALBFv3_RS04605 at 1030.555 kb on + strand, within RALBFv3_RS04605 at 1031.066 kb on + strand at 1031.069 kb on - strand at 1031.112 kb on - strand at 1031.149 kb on - strand at 1031.180 kb on - strand at 1031.193 kb on + strand at 1031.194 kb on - strand at 1031.198 kb on - strand at 1031.204 kb on + strand at 1031.205 kb on - strand at 1031.228 kb on + strand at 1031.228 kb on + strand at 1031.228 kb on + strand at 1031.324 kb on - strand at 1031.502 kb on - strand, within RALBFv3_RS04610 at 1031.940 kb on - strand, within RALBFv3_RS04610 at 1031.940 kb on - strand, within RALBFv3_RS04610 at 1031.940 kb on - strand, within RALBFv3_RS04610
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,029,462 + RALBFv3_RS04600 0.57 -0.5 1,029,487 + RALBFv3_RS04600 0.59 -1.1 1,029,488 - RALBFv3_RS04600 0.59 -1.3 1,029,896 - RALBFv3_RS04600 0.83 +0.7 1,030,018 + -1.5 1,030,018 + -0.5 1,030,036 + +0.1 1,030,037 - +1.1 1,030,169 - -0.5 1,030,293 + -0.8 1,030,425 + RALBFv3_RS04605 0.15 +0.1 1,030,426 - RALBFv3_RS04605 0.15 +1.1 1,030,468 + RALBFv3_RS04605 0.21 -1.5 1,030,468 + RALBFv3_RS04605 0.21 -0.4 1,030,469 - RALBFv3_RS04605 0.21 -0.5 1,030,469 - RALBFv3_RS04605 0.21 -0.8 1,030,469 - RALBFv3_RS04605 0.21 -0.6 1,030,469 - RALBFv3_RS04605 0.21 -1.6 1,030,511 - RALBFv3_RS04605 0.26 +1.1 1,030,511 - RALBFv3_RS04605 0.26 -0.2 1,030,555 + RALBFv3_RS04605 0.32 -1.3 1,031,066 + +0.7 1,031,069 - -0.2 1,031,112 - -0.3 1,031,149 - -0.5 1,031,180 - -0.1 1,031,193 + -0.2 1,031,194 - -1.4 1,031,198 - -0.9 1,031,204 + -0.7 1,031,205 - +0.1 1,031,228 + +0.4 1,031,228 + -1.3 1,031,228 + +0.4 1,031,324 - -1.5 1,031,502 - RALBFv3_RS04610 0.27 -0.5 1,031,940 - RALBFv3_RS04610 0.74 -0.8 1,031,940 - RALBFv3_RS04610 0.74 +0.3 1,031,940 - RALBFv3_RS04610 0.74 +2.1
Or see this region's nucleotide sequence