Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS04195 and RALBFv3_RS04200 are separated by 83 nucleotides RALBFv3_RS04200 and RALBFv3_RS04205 are separated by 301 nucleotides RALBFv3_RS04205 and RALBFv3_RS04210 are separated by 139 nucleotides
RALBFv3_RS04195: RALBFv3_RS04195 - N-acetyltransferase, at 934,876 to 936,099
_RS04195
RALBFv3_RS04200: RALBFv3_RS04200 - hypothetical protein, at 936,183 to 936,362
_RS04200
RALBFv3_RS04205: RALBFv3_RS04205 - inorganic diphosphatase, at 936,664 to 937,191
_RS04205
RALBFv3_RS04210: RALBFv3_RS04210 - aldehyde dehydrogenase, at 937,331 to 938,767
_RS04210
Position (kb) 936
937 Strain fitness (log2 ratio) -1
0
1
2 at 935.188 kb on + strand, within RALBFv3_RS04195 at 935.189 kb on - strand, within RALBFv3_RS04195 at 935.242 kb on + strand, within RALBFv3_RS04195 at 935.630 kb on - strand, within RALBFv3_RS04195 at 935.641 kb on + strand, within RALBFv3_RS04195 at 935.665 kb on + strand, within RALBFv3_RS04195 at 935.665 kb on + strand, within RALBFv3_RS04195 at 935.804 kb on - strand, within RALBFv3_RS04195 at 935.861 kb on - strand, within RALBFv3_RS04195 at 935.863 kb on + strand, within RALBFv3_RS04195 at 935.864 kb on - strand, within RALBFv3_RS04195 at 935.878 kb on + strand, within RALBFv3_RS04195 at 936.022 kb on - strand at 936.049 kb on + strand at 936.050 kb on - strand at 936.140 kb on - strand at 936.217 kb on + strand, within RALBFv3_RS04200 at 936.218 kb on - strand, within RALBFv3_RS04200 at 936.218 kb on - strand, within RALBFv3_RS04200 at 936.218 kb on - strand, within RALBFv3_RS04200 at 936.306 kb on - strand, within RALBFv3_RS04200 at 936.439 kb on + strand at 936.439 kb on + strand at 936.451 kb on + strand at 936.451 kb on + strand at 936.503 kb on - strand at 936.543 kb on + strand at 936.575 kb on - strand at 936.575 kb on - strand at 936.665 kb on + strand at 936.666 kb on - strand at 937.267 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 935,188 + RALBFv3_RS04195 0.25 +0.1 935,189 - RALBFv3_RS04195 0.26 +0.7 935,242 + RALBFv3_RS04195 0.30 -0.2 935,630 - RALBFv3_RS04195 0.62 -0.2 935,641 + RALBFv3_RS04195 0.62 +0.3 935,665 + RALBFv3_RS04195 0.64 -0.2 935,665 + RALBFv3_RS04195 0.64 +0.1 935,804 - RALBFv3_RS04195 0.76 +0.7 935,861 - RALBFv3_RS04195 0.80 -0.4 935,863 + RALBFv3_RS04195 0.81 -0.0 935,864 - RALBFv3_RS04195 0.81 -0.3 935,878 + RALBFv3_RS04195 0.82 -0.3 936,022 - -0.0 936,049 + -0.1 936,050 - -0.1 936,140 - -0.3 936,217 + RALBFv3_RS04200 0.19 -1.0 936,218 - RALBFv3_RS04200 0.19 -1.5 936,218 - RALBFv3_RS04200 0.19 +2.1 936,218 - RALBFv3_RS04200 0.19 +1.1 936,306 - RALBFv3_RS04200 0.68 -1.2 936,439 + -0.5 936,439 + -0.6 936,451 + +0.1 936,451 + -0.9 936,503 - -0.4 936,543 + -0.2 936,575 - +0.3 936,575 - +0.0 936,665 + -0.6 936,666 - +1.1 937,267 + -0.7
Or see this region's nucleotide sequence