Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS04060 and RALBFv3_RS04065 are separated by 65 nucleotides RALBFv3_RS04065 and RALBFv3_RS04070 are separated by 8 nucleotides RALBFv3_RS04070 and RALBFv3_RS04075 are separated by 3 nucleotides
RALBFv3_RS04060: RALBFv3_RS04060 - type II secretion system protein, at 907,619 to 908,131
_RS04060
RALBFv3_RS04065: RALBFv3_RS04065 - type II secretion system protein, at 908,197 to 908,598
_RS04065
RALBFv3_RS04070: RALBFv3_RS04070 - type II secretion system protein, at 908,607 to 909,125
_RS04070
RALBFv3_RS04075: RALBFv3_RS04075 - general secretion pathway protein GspD, at 909,129 to 911,453
_RS04075
Position (kb) 908
909
910 Strain fitness (log2 ratio) -2
-1
0
1 at 907.749 kb on + strand, within RALBFv3_RS04060 at 907.777 kb on - strand, within RALBFv3_RS04060 at 907.836 kb on + strand, within RALBFv3_RS04060 at 907.837 kb on - strand, within RALBFv3_RS04060 at 907.989 kb on + strand, within RALBFv3_RS04060 at 907.990 kb on - strand, within RALBFv3_RS04060 at 907.990 kb on - strand, within RALBFv3_RS04060 at 908.264 kb on + strand, within RALBFv3_RS04065 at 908.409 kb on - strand, within RALBFv3_RS04065 at 908.409 kb on - strand, within RALBFv3_RS04065 at 908.411 kb on + strand, within RALBFv3_RS04065 at 908.412 kb on - strand, within RALBFv3_RS04065 at 908.412 kb on - strand, within RALBFv3_RS04065 at 908.620 kb on + strand at 908.621 kb on - strand at 908.621 kb on - strand at 908.621 kb on - strand at 908.621 kb on - strand at 908.819 kb on + strand, within RALBFv3_RS04070 at 908.938 kb on - strand, within RALBFv3_RS04070 at 909.130 kb on + strand at 909.130 kb on + strand at 909.134 kb on - strand at 909.749 kb on + strand, within RALBFv3_RS04075 at 909.850 kb on + strand, within RALBFv3_RS04075 at 909.956 kb on - strand, within RALBFv3_RS04075 at 910.099 kb on + strand, within RALBFv3_RS04075 at 910.118 kb on - strand, within RALBFv3_RS04075
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 907,749 + RALBFv3_RS04060 0.25 -0.0 907,777 - RALBFv3_RS04060 0.31 +0.1 907,836 + RALBFv3_RS04060 0.42 -0.1 907,837 - RALBFv3_RS04060 0.42 -0.4 907,989 + RALBFv3_RS04060 0.72 +0.2 907,990 - RALBFv3_RS04060 0.72 +0.7 907,990 - RALBFv3_RS04060 0.72 +0.1 908,264 + RALBFv3_RS04065 0.17 -0.3 908,409 - RALBFv3_RS04065 0.53 -0.2 908,409 - RALBFv3_RS04065 0.53 -0.7 908,411 + RALBFv3_RS04065 0.53 -0.8 908,412 - RALBFv3_RS04065 0.53 +0.3 908,412 - RALBFv3_RS04065 0.53 -0.2 908,620 + +1.1 908,621 - -0.4 908,621 - +0.5 908,621 - -0.1 908,621 - -0.8 908,819 + RALBFv3_RS04070 0.41 -0.5 908,938 - RALBFv3_RS04070 0.64 -0.6 909,130 + +1.1 909,130 + +0.1 909,134 - -1.8 909,749 + RALBFv3_RS04075 0.27 +0.1 909,850 + RALBFv3_RS04075 0.31 -0.3 909,956 - RALBFv3_RS04075 0.36 -0.3 910,099 + RALBFv3_RS04075 0.42 +0.3 910,118 - RALBFv3_RS04075 0.43 +0.1
Or see this region's nucleotide sequence