Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS03750

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS03745 and RALBFv3_RS03750 are separated by 185 nucleotidesRALBFv3_RS03750 and RALBFv3_RS03755 are separated by 4 nucleotides RALBFv3_RS03745: RALBFv3_RS03745 - NAD(P)/FAD-dependent oxidoreductase, at 823,816 to 825,141 _RS03745 RALBFv3_RS03750: RALBFv3_RS03750 - DNA polymerase I, at 825,327 to 828,161 _RS03750 RALBFv3_RS03755: RALBFv3_RS03755 - TIGR00730 family Rossman fold protein, at 828,166 to 829,155 _RS03755 Position (kb) 825 826 827 828 829Strain fitness (log2 ratio) -1 0 1at 824.528 kb on + strand, within RALBFv3_RS03745at 824.784 kb on - strand, within RALBFv3_RS03745at 824.924 kb on + strand, within RALBFv3_RS03745at 824.953 kb on - strand, within RALBFv3_RS03745at 825.147 kb on + strandat 825.224 kb on + strandat 825.767 kb on - strand, within RALBFv3_RS03750at 826.277 kb on - strand, within RALBFv3_RS03750at 826.795 kb on + strand, within RALBFv3_RS03750at 826.868 kb on - strand, within RALBFv3_RS03750at 827.162 kb on - strand, within RALBFv3_RS03750at 827.234 kb on - strand, within RALBFv3_RS03750at 828.174 kb on + strandat 828.175 kb on - strandat 828.175 kb on - strandat 828.580 kb on + strand, within RALBFv3_RS03755at 828.616 kb on + strand, within RALBFv3_RS03755at 829.096 kb on + strandat 829.148 kb on - strandat 829.153 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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824,528 + RALBFv3_RS03745 0.54 +0.7
824,784 - RALBFv3_RS03745 0.73 +0.2
824,924 + RALBFv3_RS03745 0.84 +0.2
824,953 - RALBFv3_RS03745 0.86 -0.2
825,147 + -0.0
825,224 + +0.7
825,767 - RALBFv3_RS03750 0.16 -0.2
826,277 - RALBFv3_RS03750 0.34 -0.5
826,795 + RALBFv3_RS03750 0.52 +0.1
826,868 - RALBFv3_RS03750 0.54 +1.1
827,162 - RALBFv3_RS03750 0.65 -0.5
827,234 - RALBFv3_RS03750 0.67 -1.6
828,174 + +1.1
828,175 - -1.3
828,175 - -0.5
828,580 + RALBFv3_RS03755 0.42 -0.9
828,616 + RALBFv3_RS03755 0.45 +0.1
829,096 + +1.1
829,148 - +0.1
829,153 + -0.2

Or see this region's nucleotide sequence