Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03705 and RALBFv3_RS03710 are separated by 56 nucleotides RALBFv3_RS03710 and RALBFv3_RS03715 overlap by 4 nucleotides RALBFv3_RS03715 and RALBFv3_RS03720 are separated by 334 nucleotides
RALBFv3_RS03705: RALBFv3_RS03705 - 1-deoxy-D-xylulose-5-phosphate synthase, at 815,267 to 817,180
_RS03705
RALBFv3_RS03710: RALBFv3_RS03710 - (2E,6E)-farnesyl diphosphate synthase, at 817,237 to 818,136
_RS03710
RALBFv3_RS03715: RALBFv3_RS03715 - exodeoxyribonuclease VII small subunit, at 818,133 to 818,417
_RS03715
RALBFv3_RS03720: RALBFv3_RS03720 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 818,752 to 819,849
_RS03720
Position (kb) 818
819 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 817.218 kb on - strand at 818.351 kb on - strand, within RALBFv3_RS03715 at 818.351 kb on - strand, within RALBFv3_RS03715 at 818.351 kb on - strand, within RALBFv3_RS03715 at 818.584 kb on + strand at 818.648 kb on + strand at 818.648 kb on + strand at 818.648 kb on + strand at 818.648 kb on + strand at 818.649 kb on - strand at 818.649 kb on - strand at 818.649 kb on - strand at 818.876 kb on - strand, within RALBFv3_RS03720 at 818.914 kb on + strand, within RALBFv3_RS03720 at 819.070 kb on + strand, within RALBFv3_RS03720 at 819.241 kb on + strand, within RALBFv3_RS03720 at 819.245 kb on + strand, within RALBFv3_RS03720 at 819.277 kb on + strand, within RALBFv3_RS03720 at 819.278 kb on - strand, within RALBFv3_RS03720 at 819.305 kb on - strand, within RALBFv3_RS03720
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 817,218 - +0.0 818,351 - RALBFv3_RS03715 0.76 -1.4 818,351 - RALBFv3_RS03715 0.76 -1.1 818,351 - RALBFv3_RS03715 0.76 -1.3 818,584 + -0.1 818,648 + -0.2 818,648 + +0.8 818,648 + -0.1 818,648 + +0.2 818,649 - -1.8 818,649 - -1.9 818,649 - -1.5 818,876 - RALBFv3_RS03720 0.11 +1.1 818,914 + RALBFv3_RS03720 0.15 -3.5 819,070 + RALBFv3_RS03720 0.29 -0.5 819,241 + RALBFv3_RS03720 0.45 -2.3 819,245 + RALBFv3_RS03720 0.45 -2.3 819,277 + RALBFv3_RS03720 0.48 -2.2 819,278 - RALBFv3_RS03720 0.48 -2.6 819,305 - RALBFv3_RS03720 0.50 -2.2
Or see this region's nucleotide sequence