Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03595 and RALBFv3_RS03600 are separated by 48 nucleotides RALBFv3_RS03600 and RALBFv3_RS03605 are separated by 247 nucleotides
RALBFv3_RS03595: RALBFv3_RS03595 - lipid A export permease/ATP-binding protein MsbA, at 787,496 to 789,274
_RS03595
RALBFv3_RS03600: RALBFv3_RS03600 - glycosyl transferase, at 789,323 to 790,183
_RS03600
RALBFv3_RS03605: RALBFv3_RS03605 - glycosyltransferase family 2 protein, at 790,431 to 791,201
_RS03605
Position (kb) 789
790
791 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 789.456 kb on + strand, within RALBFv3_RS03600 at 789.457 kb on - strand, within RALBFv3_RS03600 at 789.457 kb on - strand, within RALBFv3_RS03600 at 789.457 kb on - strand, within RALBFv3_RS03600 at 789.555 kb on + strand, within RALBFv3_RS03600 at 789.661 kb on - strand, within RALBFv3_RS03600 at 789.810 kb on - strand, within RALBFv3_RS03600 at 789.810 kb on - strand, within RALBFv3_RS03600 at 789.943 kb on - strand, within RALBFv3_RS03600 at 789.951 kb on + strand, within RALBFv3_RS03600 at 789.951 kb on + strand, within RALBFv3_RS03600 at 789.951 kb on + strand, within RALBFv3_RS03600 at 789.952 kb on - strand, within RALBFv3_RS03600 at 790.021 kb on + strand, within RALBFv3_RS03600 at 790.022 kb on - strand, within RALBFv3_RS03600 at 790.170 kb on - strand at 790.183 kb on + strand at 790.183 kb on + strand at 790.183 kb on + strand at 790.184 kb on - strand at 790.195 kb on + strand at 790.196 kb on - strand at 790.249 kb on + strand at 790.343 kb on + strand at 790.344 kb on - strand at 790.821 kb on + strand, within RALBFv3_RS03605 at 790.822 kb on - strand, within RALBFv3_RS03605 at 790.932 kb on + strand, within RALBFv3_RS03605 at 790.933 kb on - strand, within RALBFv3_RS03605 at 790.939 kb on - strand, within RALBFv3_RS03605 at 790.939 kb on - strand, within RALBFv3_RS03605
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 789,456 + RALBFv3_RS03600 0.15 -1.9 789,457 - RALBFv3_RS03600 0.16 -2.3 789,457 - RALBFv3_RS03600 0.16 -1.5 789,457 - RALBFv3_RS03600 0.16 -2.3 789,555 + RALBFv3_RS03600 0.27 +1.1 789,661 - RALBFv3_RS03600 0.39 -3.3 789,810 - RALBFv3_RS03600 0.57 +0.1 789,810 - RALBFv3_RS03600 0.57 -3.8 789,943 - RALBFv3_RS03600 0.72 -3.3 789,951 + RALBFv3_RS03600 0.73 -2.2 789,951 + RALBFv3_RS03600 0.73 -3.0 789,951 + RALBFv3_RS03600 0.73 -2.9 789,952 - RALBFv3_RS03600 0.73 -4.6 790,021 + RALBFv3_RS03600 0.81 -0.5 790,022 - RALBFv3_RS03600 0.81 -2.4 790,170 - -2.6 790,183 + -2.3 790,183 + -0.3 790,183 + -0.8 790,184 - +0.9 790,195 + -0.1 790,196 - +0.5 790,249 + -1.3 790,343 + -1.0 790,344 - -3.3 790,821 + RALBFv3_RS03605 0.51 -3.5 790,822 - RALBFv3_RS03605 0.51 -4.0 790,932 + RALBFv3_RS03605 0.65 -2.7 790,933 - RALBFv3_RS03605 0.65 +0.1 790,939 - RALBFv3_RS03605 0.66 -1.3 790,939 - RALBFv3_RS03605 0.66 +1.1
Or see this region's nucleotide sequence