Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03430 and RALBFv3_RS03435 overlap by 4 nucleotides RALBFv3_RS03435 and RALBFv3_RS03440 are separated by 262 nucleotides
RALBFv3_RS03430: RALBFv3_RS03430 - allophanate hydrolase, at 755,694 to 756,755
_RS03430
RALBFv3_RS03435: RALBFv3_RS03435 - allophanate hydrolase, at 756,752 to 757,399
_RS03435
RALBFv3_RS03440: RALBFv3_RS03440 - Bcr/CflA family drug resistance efflux transporter, at 757,662 to 758,885
_RS03440
Position (kb) 756
757
758 Strain fitness (log2 ratio) -1
0
1 at 756.139 kb on + strand, within RALBFv3_RS03430 at 756.388 kb on + strand, within RALBFv3_RS03430 at 756.542 kb on + strand, within RALBFv3_RS03430 at 756.543 kb on - strand, within RALBFv3_RS03430 at 756.610 kb on + strand, within RALBFv3_RS03430 at 756.611 kb on - strand, within RALBFv3_RS03430 at 756.755 kb on + strand at 756.755 kb on + strand at 756.889 kb on - strand, within RALBFv3_RS03435 at 756.889 kb on - strand, within RALBFv3_RS03435 at 756.993 kb on + strand, within RALBFv3_RS03435 at 756.994 kb on - strand, within RALBFv3_RS03435 at 757.032 kb on + strand, within RALBFv3_RS03435 at 757.476 kb on + strand at 757.505 kb on + strand at 757.512 kb on + strand at 757.513 kb on - strand at 757.568 kb on + strand at 757.568 kb on + strand at 757.568 kb on + strand at 757.569 kb on - strand at 757.571 kb on + strand at 757.572 kb on - strand at 757.572 kb on - strand at 757.617 kb on + strand at 757.907 kb on - strand, within RALBFv3_RS03440 at 757.907 kb on - strand, within RALBFv3_RS03440 at 758.104 kb on + strand, within RALBFv3_RS03440 at 758.104 kb on + strand, within RALBFv3_RS03440 at 758.105 kb on - strand, within RALBFv3_RS03440 at 758.105 kb on - strand, within RALBFv3_RS03440 at 758.105 kb on - strand, within RALBFv3_RS03440 at 758.126 kb on - strand, within RALBFv3_RS03440 at 758.162 kb on - strand, within RALBFv3_RS03440 at 758.162 kb on - strand, within RALBFv3_RS03440 at 758.201 kb on + strand, within RALBFv3_RS03440 at 758.201 kb on + strand, within RALBFv3_RS03440 at 758.203 kb on + strand, within RALBFv3_RS03440 at 758.203 kb on + strand, within RALBFv3_RS03440 at 758.204 kb on - strand, within RALBFv3_RS03440 at 758.204 kb on - strand, within RALBFv3_RS03440
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 756,139 + RALBFv3_RS03430 0.42 -0.3 756,388 + RALBFv3_RS03430 0.65 -1.1 756,542 + RALBFv3_RS03430 0.80 +0.5 756,543 - RALBFv3_RS03430 0.80 +0.3 756,610 + RALBFv3_RS03430 0.86 -0.6 756,611 - RALBFv3_RS03430 0.86 -0.2 756,755 + -0.0 756,755 + -0.7 756,889 - RALBFv3_RS03435 0.21 -0.6 756,889 - RALBFv3_RS03435 0.21 -1.2 756,993 + RALBFv3_RS03435 0.37 -1.0 756,994 - RALBFv3_RS03435 0.37 -0.9 757,032 + RALBFv3_RS03435 0.43 +0.1 757,476 + -0.8 757,505 + -0.5 757,512 + +0.2 757,513 - +0.1 757,568 + -0.4 757,568 + +0.1 757,568 + -0.2 757,569 - +1.5 757,571 + -0.9 757,572 - +0.6 757,572 - -0.5 757,617 + +0.3 757,907 - RALBFv3_RS03440 0.20 +1.4 757,907 - RALBFv3_RS03440 0.20 +0.6 758,104 + RALBFv3_RS03440 0.36 +0.9 758,104 + RALBFv3_RS03440 0.36 -0.2 758,105 - RALBFv3_RS03440 0.36 -0.2 758,105 - RALBFv3_RS03440 0.36 +1.1 758,105 - RALBFv3_RS03440 0.36 +0.8 758,126 - RALBFv3_RS03440 0.38 -0.7 758,162 - RALBFv3_RS03440 0.41 +0.1 758,162 - RALBFv3_RS03440 0.41 -0.8 758,201 + RALBFv3_RS03440 0.44 -0.2 758,201 + RALBFv3_RS03440 0.44 +0.5 758,203 + RALBFv3_RS03440 0.44 +0.1 758,203 + RALBFv3_RS03440 0.44 -0.2 758,204 - RALBFv3_RS03440 0.44 -0.5 758,204 - RALBFv3_RS03440 0.44 -0.0
Or see this region's nucleotide sequence